A novel upstream RNA polymerase III promoter element becomes essential when the chromatin structure of the yeast U6 RNA gene is altered

Molecular and Cellular Biology

Volumn 21, Issue 19, 2001, Pages 6429-6439

  Martin, M P ^a   Gerlach, V L ^a   Brow, D A ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; DNA DIRECTED RNA POLYMERASE III; RNA; TRANSCRIPTION FACTOR;

ALLELE; ARTICLE; BASE PAIRING; CHROMATIN STRUCTURE; CONTROLLED STUDY; GENE; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SNR6 GENE; START CODON; TATA BOX; TRANSCRIPTION INITIATION;

AT RICH SEQUENCE; CHROMATIN; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; MUTATION; NUCLEAR PROTEINS; PROMOTER REGIONS (GENETICS); RESPONSE ELEMENTS; RNA POLYMERASE III; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TATA BOX; TRANSCRIPTION FACTOR TFIIIB; TRANSCRIPTION FACTORS; TRANSCRIPTION FACTORS, TFIII; TRANSCRIPTION, GENETIC;

EID: 0034804387     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.19.6429-6439.2001     Document Type: Article

References (48)

1. Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC
2. Transcription of a yeast U6 snRNA gene requires a polymerase III promoter element in a novel position
3. Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters
4. TFIIIC relieves repression of U6 snRNA transcription by chromatin
5. Architecture of protein and DNA contacts within the TFIIIB-DNA complex
6. NHP6A and NHP6B, which encode HMG1-like proteins, are candidates for downstream components of the yeast SLT2 mitogen-activated protein kinase pathway
7. TFIIIC-independent in vitro transcription of yeast tRNA genes
8. Architecture of a yeast U6 RNA gene promoter
9. The RNA polymerase III transcription apparatus
10. TFIIIB placement on a yeast U6 RNA gene in vivo is directed primarily by TFIIIC rather than by sequence-specific DNA contacts
11. Ribonuclease protection assay
12. The RNA polymerase III-recruiting factor TFIIIB induces a DNA bend between the TATA box and the transcriptional start site
13. Poly(dA:dT), a ubiquitous promoter element that stimulates transcription via its intrinsic DNA structure
14. Identical components of yeast transcription factor IIIB are required and sufficient for transcription of TATA box-containing and TATA-less genes
15. Lethal mutations in a yeast U6 RNA gene B block promoter element identify essential contacts with transcription factor-IIIC
16. The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening
17. DNA bending at adenine and thymine tracts
18. Nhp6: An HMG1 protein, functions in SNR6 transcription by RNA polymerase III in S. cerevisiae
19. Human TFIIIC relieves chromatin-mediated repression of RNA polymerase III transcription and contains an intrinsic histone acetyltransferase activity
20. Nucleosomes will not form on double-stranded RNA or over poly(dA)-poly(dT) tracts in recombinant DNA
21. Rapid and efficient site-specific mutagenesis without phenotypic selection
22. Expression of RNase P RNA in Saccharomyces cerevisiae is controlled by an unusual RNA polymerase III promoter
23. Differential expression of individual suppressor tRNA(Trp) gene gene family members in vitro and in vivo in the nematode Caenorhabditis elegans
24. The TATA element and its context affect the cooperative interaction of TATA-binding protein with the TFIIB-related factor TFIIIB70
25. High-mobility-group proteins NHP6A and NHP6B participate in activation of the RNA polymerase III SNR6 gene
26. Multiple roles for U6 snRNA in the splicing pathway
27. Reciprocal interferences between nucleosomal organization and transcriptional activity of the yeast SNR6 gene
28. The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro
29. In vitro transcription of a silkworm 5S RNA gene requires an upstream signal
30. Transcription of a silkworm tRNA(cAla) gene is directed by two AT-rich upstream sequence elements
31. Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of pre-initiation complexes in vitro
32. Spatial organization of the core region of yeast TFIIIB-DNA complexes
33. Nucleosome reconstitution on plasmid-inserted poly(dA)poly(dT)
34. TFIID sequence recognition of the initiator and sequences farther downstream in Drosophila class II genes
35. TFIIIC determines RNA polymerase III specificity at the TATA-containing yeast U6 promoter
36. A Saccharomyces cerevisiae genomic plasmid bank based on a centromerecontaining shuttle vector
37. A differential response of wild type and mutant promoters to TFIIIB70 overexpression in vivo and in vitro
38. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
39. 5′ flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems
40. Mechanism of transcription through the nucleosome by eukaryotic RNA polymerase
41. A TATA element is required for tRNA promoter activity and confers TATA-binding protein responsiveness in Drosophila Schneider-2 cells
42. Production of single-stranded plasmid DNA
43. The symmetry of the yeast U6 RNA gene's TATA box and the orientation of the TATA-binding protein in yeast TFIIIB
44. Yeast contains small nuclear RNAs encoded by single copy genes
45. Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro
46. TBP-DNA interactions in the minor groove discriminate between A:T and T:A base pairs
47. Determinants of DNA binding and bending by the Saccharomyces cerevisiae high mobility group protein NHP6A that are important for its biological activities. Role of the unique N terminus and putative intercalating methionine
48. A specialized nucleosome modulates transcription factor access to a C. glabrata metal responsive promoter