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Volumn 278, Issue 5345, 1997, Pages 1960-1963

Mechanism of transcription through the nucleosome by eukaryotic RNA polymerase

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA DIRECTED RNA POLYMERASE III; RNA;

EID: 0031451329     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5345.1960     Document Type: Article
Times cited : (176)

References (30)
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    • _, ibid. 83, 19 (1995).
    • (1995) Cell , vol.83 , pp. 19
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    • V. M. Studitsky, G. A. Kassavetis, E. P. Geiduschek, G. Felsenfeld, data not shown
    • V. M. Studitsky, G. A. Kassavetis, E. P. Geiduschek, G. Felsenfeld, data not shown.
  • 22
    • 0008696879 scopus 로고
    • A. Wolffe, Ed. JAI Press, Greenwich, CT
    • D. J. Clark, in The Nucleus, A. Wolffe, Ed. (JAI Press, Greenwich, CT, 1995), pp. 207-239.
    • (1995) The Nucleus , pp. 207-239
    • Clark, D.J.1
  • 24
    • 2642661858 scopus 로고    scopus 로고
    • note
    • Initiation (-NTP) and early elongation (-GTP) complexes were not detected, probably because of instability during electrophoresis.
  • 26
    • 2642659789 scopus 로고    scopus 로고
    • note
    • To isolate nucleosomes positioned at the Nco I end, reconstitutes were incubated in the presence of 10 U of Hae II restriction enzyme in SP6 transcription buffer at 20°C for 45 min and separated from free DNA and differently positioned cores in preparative nucleoprotein gels.
  • 28
    • 2642589407 scopus 로고    scopus 로고
    • note
    • 2, and 1 mM DTT at 37°C for 20 min. The size distributions of radioactive RNA were quantified with a Phosphorlmager.
  • 30
    • 2642656691 scopus 로고    scopus 로고
    • note
    • We thank D. Clark for comments on the manuscript. Support of research at the University of California, San Diego by a grant from the National Institute of General Medical Sciences is gratefully acknowledged.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.