The experimental use of antisense oligonucleotides: A guide for the perplexed

Journal of Clinical Investigation

Volumn 108, Issue 5, 2001, Pages 641-644

  Stein, C A ^a  

^a Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; CD4 ANTIGEN; DNA; FIBRONECTIN; GROWTH FACTOR; HEPARIN BINDING PROTEIN; LAMININ; MESSENGER RNA; PHOSPHOROTHIOIC ACID DERIVATIVE; RNA;

ANIMAL EXPERIMENT; BIOTECHNOLOGY; GENE EXPRESSION REGULATION; GENE FUNCTION; HUMAN; HUMAN TISSUE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SCID MOUSE; SHORT SURVEY; XENOGRAFT;

EID: 0034798245     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI13885     Document Type: Short Survey

References (24)

1. Problems in interpretation of data derived from in vitro and in vivo use of antisense oligodeoxynucleotides
2. Physicochemical properties of phosphorothioate oligodeoxynucleotides
3. Phosphorothioate oligodeoxynucleotides bind to basic fibroblast growth factor, inhibit its binding to cell surface receptors, and remove it from low affinity binding sites on extracellular matrix
4. Inhibition of high affinity basic fibroblast growth factor binding by oligonucleotides
5. Cell-surface perturbations of the epidermal growth factor and vascular endothelial growth factor receptors by phosphorothioate oligodeoxynucleotides
6. Polydeoxyguanine motifs in a 12-mer phosphorothioate oligodeoxynucleotide augment binding to the v3 loop of HIV-1 gp120 and potency of HIV-1 inhibition independency of G-tetrad formation
7. Mac-1 (CD11b/CD18) is an oligodeoxynucleotide-binding protein
8. Multiple mechanisms may contribute to the cellular anti-adhesive effects of phosphorothioate oligodeoxynucleotides
9. Dynamics of the internalization of phosphodiester oligodeoxynucleotides in HL60 cells
10. Role of RNase H in hybrid-arrested translation by antisense oligonucleotides
11. RNase H cleavage of RNA hybridized to oligonucleotides containing methylphosphonate, phosphorothioate and phosphodiester bonds
12. Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents
13. Evaluation of 2′-modified oligonucleotides containing 2′-deoxy gaps as antisense inhibitors of gene expression
14. Tetranucleotide GGGA motif in primary RNA transcripts. Novel target site for antisense design
15. Inhibition of protein kinase C-alpha expression in human A549 cells by antisense oligonucleotides inhibits induction of intercellular adhesion molecule 1 (ICAM-1) mRNA by phorbol esters
16. Antitumor activity of a phosphorothioate antisense oligodeoxynucleotide targeted against C-raf kinase
17. Bcl-xL in prostate cancer cells: Effects of overexpression and down- Regulation on chemosensitivity
18. Andsense oligodeoxyribonucleotides suppress hematologic cell growth through stepwise release of deoxyribonucleotides
19. Cationic lipids enhance cellular uptake and activity, of phosphorothioate antisense oligonucleotides
20. Novel DNA superstructures formed by telomere-like oligomers
21. Formation of a G-tetrad and higher order structures correlates with biological activity of the RelA (NF-kappaB p65) 'antisense' oligodeoxynucleotide
22. CpG motifs in bacterial DNA trigger direct B-cell activation
23. Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA
24. CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma