Structure of E. coli 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase reveals similarity to the purine nucleoside phosphorylases

Structure

Volumn 9, Issue 10, 2001, Pages 941-953

  Lee, J E ^a   Cornell, K A ^a   Riscoe, M K ^a   Howell, P L ^a  

^a HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

Author keywords

5 Methylthioadenosine S adenosylhomocysteine nucleosidase; Enzyme mechanism; Methionine recycling pathway; Methylthioadenosine phosphorylase; Purine nucleoside phosphorylase; Structure based drug design

Indexed keywords

5' METHYLTHIOADENOSINE; 5' METHYLTHIORIBOSE; ADENINE; ADENOSYLHOMOCYSTEINE NUCLEOSIDASE; AMINO ACID; CYSTEINE DERIVATIVE; DIMER; HYDROLASE; METHYLTHIOADENOSINE NUCLEOSIDASE; MONOMER; NUCLEOSIDASE; NUCLEOSIDE N RIBOHYDROLASE; PURINE NUCLEOSIDE PHOSPHORYLASE; RIBOSE; S ADENOSYLHOMOCYSTEINE; S RIBOSYLHOMOCYSTEINE; THIORIBOSE; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIFFRACTION; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME METABOLISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; EUKARYOTE; HYDROPHOBICITY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; PROTEIN QUATERNARY STRUCTURE; SEQUENCE HOMOLOGY;

ADENINE; AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; N-GLYCOSYL HYDROLASES; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; PURINE-NUCLEOSIDE PHOSPHORYLASE; RIBOSE; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; MAMMALIA; PROKARYOTA;

EID: 0034793650     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00656-6     Document Type: Article

References (56)

1. A hydrolytic nucelosidase acting on S-adenosylhomocysteine and on 5′-methylthioadenosine
2. Cloning and expression of Escherichia coli 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase: Identification of the pfs gene product
3. Characterization of recombinant Escherichia coli 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase: Analysis of enzymatic activity and substrate specificity
4. Methionine recycling pathways and antimalarial drug design
5. Methionine recycling as a target for antiprotozoal drug development
6. The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine
7. S-Adenosyl-L-methionine-dependent macromolecule methyltransferases: Potential targets for the design of chemotherapeutic agents
8. Methylthioadenosine, a potent inhibitor of spermine synthase from bovine brain
9. Inhibition of the synthesis of polyamines and macromolecules by 5′-methylthioadenosine and 5′-alkylthiotubercidins in BHK21 cells
10. Mechanism of action of 5′-methylthioadenosine in S49 cells
11. Inhibition of cytokine production and endothelial expression of adhesion antigens by 5′-methylthioadenosine
12. Affinity purification of 5-methylthioribose kinase and 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae
13. Kinetic properties and the effect of substrate analogues on 5′-methylthioadenosine nucleosidase from Escherichia coli
14. 5′-Methylthioadenosine nucleosidase. Purification and characterization of the enzyme from Lupinus luteus seeds
15. Escherichia coli S-adenosylhomocysteine/5′-methylthioadenosine nucleosidase. Purification, substrate specificity and mechanism of action
16. Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 Å resolution
17. Expression, purification, crystallization and preliminary X-ray analysis of Escherichia coli 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase
18. Comparison of super-secondary structures in proteins
19. Structure and function of S-adenosylhomocysteine hydrolase
20. Structure determination of selenomethionyl S-adenosylhomocysteine hydrolase using data at a single wavelength
21. Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata
22. Substrate specificities of 5′-deoxy-5′-methylthioadenosine phosphorylase from Trypanosoma brucei brucei and mammalian cells
23. Purification and characterization of 5′-deoxy-5′-methylthioadenosine (MTA) phosphorylase from human liver
24. Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 Å resolution
25. Crystal structure of the purine nucleoside phosphorylase (PNP) from Cellulomonas sp. and its implication for the mechanism of trimeric PNPs
26. The structure of human 5′-deoxy-5′-methylthioadenosine phosphorylase at 1.7 Å resolution provides insights into substrate binding and catalysis
27. Two purine nucleoside phosphorylases in Bacillus subtilis. Purification and some properties of the adenosine-specific phosphorylase
28. The crystal structure of Escherichia coli purine nucleoside phosphorylase: A comparison with the human enzyme reveals a conserved topology
29. Atomic structure at 2.5 Å resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice
30. Crystal structure of the ternary complex of E. coli purine nucleoside phosphorylase with formycin B, a structural analogue of the substrate inosine, and phosphate (Sulphate) at 2.1 Å resolution
31. The catalytic mechanism of adenosylhomocysteine/methylthioadenosine nucleosidase from Escherichia coli-chemical evidence for a transition state with a substantial oxocarbenium character
32. Aromatic-aromatic interaction: A mechanism of protein structure stabilization
33. A structural analysis of phosphate and sulphate binding sites in proteins. Estimation of propensities for binding and conservation of phosphate binding sites
34. Purine nucleoside phosphorylase. 2. Catalytic mechanism
35. Transition state structure of purine nucleoside phosphorylase and principles of atomic motion in enzymatic catalysis
36. On the catalytic mechanism of adenosylhomocysteine/methylthioadenosine nucleosidase from E. coli
37. Transition-state analysis of nucleoside hydrolase from Crithidia fasciculata
38. The conserved, buried aspartic acid in oxidized Escherichia coli thioredoxin has a pKa of 7.5. Its titration produces a related shift in global stability
39. Processing of X-ray diffraction data collected in oscillation mode
40. The finer things in X-ray diffraction data collection
41. Crystallography and NMR system: A new software suite for macromolecular structure determination
42. A highly automated heavy-atom search procedure for macromolecular structures
43. Treatment of multiwavelength anomalous diffraction data as a special case of multiple isomorphous replacement
44. XtalView/Xfit - A versatile program for manipulating atomic coordinates and electron density
45. Slow-cooling protocols for crystallographic refinement by simulated annealing
46. Procheck: A program to check the stereochemical quality of protein structures
47. Stereochemical quality of protein structure coordinates
48. Setor: Hardware-lighted three-dimensional solid model representations of macromolecules
49. Promotif - A program to identify and analyze structural motifs in proteins
50. Molscript: A program to produce both detailed and schematic plots of protein structures
51. Raster3D: Photorealistic molecular graphics
52. Motif-based searching in tops protein topology databases
53. Protein structural topology: Automated analysis and diagrammatic representation
54. An atlas of protein topology cartoons available on the world-wide web
55. VMD-visual molecular dynamics