Yeast LrgIp acts as a specialized RhoGAP regulating 1,3-β-glucan synthesis

Yeast

Volumn 18, Issue 10, 2001, Pages 943-951

  Watanabe, Daisuke ^a   Abe, Mitsuhiro ^a   Ohya, Yoshikazu ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

1,3 glucan; Fks1p; GTPase activating proteins (GAPs); Lrg1p; Pkc1p; Rholp; Saccharomyces cerevisiae

Indexed keywords

1,3 BETA GLUCAN SYNTHASE; BETA 1,3 GLUCAN; FUNGAL PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; LRG1 PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE; UNCLASSIFIED DRUG; BETA GLUCAN; BETA-1,3-GLUCAN; DNA BINDING PROTEIN; FKS1 PROTEIN, S CEREVISIAE; GLUCAN; GLUCOSYLTRANSFERASE; LRG1 PROTEIN, S CEREVISIAE; MEMBRANE PROTEIN; PKC1 PROTEIN, TRICHODERMA REESEI; PROTEIN KINASE C; RHO GTPASE ACTIVATING PROTEIN; RHO GTPASE-ACTIVATING PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHO1 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; FUNGUS MUTANT; GENE MUTATION; GENE REPRESSION; GENE SEQUENCE; GENETIC CONSERVATION; GENETIC SELECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN SYNTHESIS; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; TEMPERATURE SENSITIVITY; TWO HYBRID SYSTEM; YEAST; BIOSYNTHESIS; GENE EXPRESSION REGULATION; METABOLISM; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

BETA-GLUCANS; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GLUCANS; GLUCOSYLTRANSFERASES; GTPASE-ACTIVATING PROTEINS; MEMBRANE PROTEINS; PROTEIN KINASE C; RHO GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0034742648     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.742     Document Type: Article

References (35)

1. Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein β subunits and the yeast response regulator protein Skn7
2. The LIM domain: Regulation by association
3. Proteins regulating Ras and its relatives
4. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae
5. Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae
6. RGD1 genetically interacts with MID2 and SLG1, encoding two putative sensors for cell integrity signalling in Saccharomyces cerevisiae
7. Cell wall stress depolarizes cell growth via hyperactivation of RHO1
8. The yeast Rgd1p is a GTPase activating protein of the Rho3 and Rho4 proteins
9. Rho1p, a yeast protein at the interface between cell polarization and morphogenesis
10. Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis
11. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast
12. The Rho1 effector Pkc1, but not Bnil, mediates signalling from Tor2 to the actin cytoskeleton
13. Prenylation of Rho1p is required for activation of yeast 1,3-β-glucan synthase
14. Activation of yeast protein kinase C by Rho1
15. Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae
16. Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae
17. Regulatory mechanisms for modulation of signalling through the cell integrity Slt2-mediated pathway in Saccharomyces cerevisiae
18. In vitro activity of 1,3-β-D-glucan synthase requires the GTP-binding protein Rho1
19. LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains
20. A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae
21. Biogenesis of yeast wall and surface components
22. Rom1p and Rom2p are GDP/GTP exchange proteins (GEPs) for the Rho1p small GTP binding protein in Saccharomyces cerevisiae
23. Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast
24. Wsc1 and Mid2 are cell surface sensors for cell wall integrity signalling that act through Rom2, a guanine nucleotide exchange factor for Rho1
25. Fluorescence microscopy methods in yeast
26. Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-β-glucan synthase
27. A series of protein phosphatase gene disruptants in Saccharomyces cerevisiae
28. A microtiter-based fluorescence assay for (1,3)-β-glucan synthases
29. The yeast phosphatidylinositol kinase homology TOR2 activates RHO1 and RHO2 via the exchange factor ROM2
30. Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae
31. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
32. Control of the yeast budsite assembly GTPase Cdc42
33. Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins