Characterization of mutant HIV-1 integrase carrying amino acid changes in the catalytic domain

Molecules and Cells

Volumn 10, Issue 5, 2000, Pages 525-532

  Sayasith, Khampoune ^a,b   Sauvé, Gilles ^a   Yelle, Jocelyn ^a  

^a INRS INSTITUT ARMAND FRAPPIER   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

HIV 1 integrase; Integrase activity; Integrase mutants; Lntegrase catalytic domain; Site directed mutagenesis

Indexed keywords

HUMAN IMMUNODEFICIENCY VIRUS 1;

INTEGRASE; OLIGODEOXYRIBONUCLEOTIDE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CHEMISTRY; CONFORMATION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; CLONING, MOLECULAR; CONSERVED SEQUENCE; ESCHERICHIA COLI; HIV INTEGRASE; HIV-1; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; OLIGODEOXYRIBONUCLEOTIDES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 0034739559     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10059-000-0525-3     Document Type: Article

References (28)

1. Brown, P. O., Bowerman, B., Varmus, H. E., and Bishop, J. M. (1989) Retroviral integration: structure of initial covalent product and its precursor, and a role for the viral IN protein. Proc. Natl. Acad. Sci. USA 86, 2525-2529.
2. Bushman, F. D., Engelman, A., Palmer, I., Wingfield, P., and Craigie, R. (1993) Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proc. Natl. Acad. Sci. USA 90, 3428-3432.
3. Chow, S. A., Vincent, K. A., Ellison, V., and Brown, P. O. (1992) Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science 255, 723-726.
4. Craigie, R., Hickman, A. B., and Engelman, A. (1995) Integrase: in HIV, Volume II: A practical approach, J. Karn (ed), pp. 53-71, Oxford University Press.
5. Drelich, M., Wilhelm, R., and Mous, J. (1992) Identification of amino acid residues critical forendonuclease and integration activities of HIV-1 IN protein in vitro. Virology 188, 459-468.
6. Engelman, A. and Craigie, R. (1992) Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J. Virol. 66, 6361-6169.
7. Engelman, A., Englund, G., Orenstein, J. M., Martin, M. A., and Craigie, R. (1995) Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication. J. Virol. 69, 2729-2736.
8. Farnet, C. and Haseltine, W. A. (1990) Integration of human immunodeficiency virus type 1 (HIV-1) DNA in vitro. Proc. Natl. Acad. Sci. USA 87, 4164-4168.
9. Fujiwara, T. and Mizuuchi, K. (1988) Retroviral DNA integration: structure of an integration intermediate. Cell 54, 497-504.
10. Gerton, J. L., Ohgi, S., Olsen, M., DeRisi, J., and Brown, P. O. (1998) Effect of mutations in residues near the active site of human immunodeficiency virus type 1 inlegrase on specifc enzyme-substrate interaction. J. Virol. 72, 5046-5055.
11. Goff, S. P. (1992) Genetic of retroviral integration. Annu. Rev. Genet. 26, 527-544.
12. Goulaouic, H. and Chow, S. A. (1996) Directed integration of viral DNA mediated by fusion protein consisting of human immunodeficiency virus type-1 integrase and Escherichia coli LexA protein. J. Virol. 73, 37-46.
13. Heuer, T. S. and Brown, P. O. (1998) Photo-cross-linking studies suggest a model for the architecture of an active human immunodeficiency virus type 1 integrase-DNA-complex. Biochemistry 37, 6667-6678.
14. Jenkins, T. M., Engelman, A., Ghirlando, R., and Craigie, R. (1996) A soluble mutant of HIV-1 integrase: involvement of both the core and C-terminal domains in multimerization. J. Biol. Chem. 271, 7712-7718.
15. Jenkins, T. M., Esposito, D., Engelman, A., and Craigie, R. (1997) Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photocrosslinking. EMBO J. 16, 849-6859.
16. Katz, R. A. and Skalka, A. M. (1994) The retroviral enzymes. Annu. Rev. Biochem. 63, 133-173.
17. Kulkosky, J., Jones, K. S., Katz, R. A., Mack, J. P. G., and Skalka, A. M. (1992) Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral. retrotransposon integrases and bacterial insertion sequence transposase. Mol. Cell. Biol. 12, 2331-2338.
18. Leavitt, A. D., Shuie, L., and Varmus, H. E. (1993) Site-directed mutagenesis of HIV-1 integrase demonstrated differential effects on integrase function in vitro. J. Biol. Chem. 268, 2113-2119.
19. Lutzke, R. A. P., Eppens, N. A., Weber, P. A., Houghten, R. A., and Plasterk, R. H. A. (1995) Identification of a hexapeptide inhibitor of the human immunodeficiency virus integrase protein by using a combination chemical library. Proc. Natl. Acad, Sci. USA 92, 11456-11460.
20. Maignan, S., Guilloteau, J. P., Zhou-Liu, Q., and Mikol, V. (1998) Crystal structure of the catalytic domain of HIV-1 integrase free and complexed with its meta cofactor: high level of similarity of the active site with other viral integrases. J. Mol. Biol. 282, 359-368.
21. Pommier, Y., Pilan, A. A., Bajaj, K., Mazumder, A., and Neamati, N. (1997) HIV-1 integrase as a target for antiviral drugs. Antiviral Chemistry & Chemotherapy 8, 463-483.
22. Pahl, A. and Flügel, R. M. (1995) Characterization of human spuma retrovirus integrase by site-directed mutagenesis, by complementation, and by swapping the zinc finger domain of HIV-1. J. Biol. Chem. 270, 2857-2966.
23. Roth, M. J., Schwartzenberg, P. L., and Goff, S. P. (1989) Structure of the termini of DNA intermediates in the integration of retroviral DNA: dependence of IN function and terminal DNA sequence. Cell 58, 47-54.
24. Stevenson, M., Haggerty, S., Lamonica, C. A., Me ter, C. M., Welch, S. K., and Wasiak, A. J. (1990) Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. J. Virol. 64, 2121-2125.
25. van den Ent, F. M., Vos, A., and Plasterk, R. H. (1998) Mutational scan of the human immunodeficiency virus type 2 integrase protein. J. Virol. 72, 3916-3924.
26. van Gent, D. C., Vink, C., Oude Groeneger, A. A. M., and Plasterk, R. H. A. (1993) Complementation between HIV integrase proteins mutated in different domains. EMBO J. 12, 3261-3267.
27. Wiskerchen, M. and Muesing, M.A. (1995) Human immunodeficiency virus type 1 integrase: effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells. J. Virol. 69, 376-386.
28. Woerner, A. M., Klutch, M., Levin, J. G., and Marcus-Sekura, C. J. (1992) Localization of HIV-1 integrase to the C-terminal half of the protein. AIDS Res. Hum. Retroviruses 8, 297-304.