Post-translational modification of p53 protein in response to ionizing radiation analyzed by mass spectrometry

Journal of Molecular Biology

Volumn 295, Issue 4, 2000, Pages 853-864

  Abraham, Jacinth ^a   Kelly, John ^b   Thibault, Pierre ^b   Benchimol, Sam ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Acetylation; Mass spectrometry; p53; Phosphorylation; Post translational

Indexed keywords

PROTEIN P53; TRANSCRIPTION FACTOR;

ACETYLATION; APOPTOSIS; ARTICLE; COVALENT BOND; DNA DAMAGE; DNA SEQUENCE; HUMAN; HUMAN CELL; IONIZING RADIATION; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; STRESS; TRANSCRIPTION REGULATION;

EID: 0034723153     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3415     Document Type: Article

References (64)

1. Phosphorylation of p53 protein in response to ionizing radiation occurs at multiple sites in both normal and DNA-PK deficient cells
2. Conformation-dependent phosphorylation of p53
3. Protein kinases and the response to DNA damage
4. Regulation of p53 function and stability by phosphorylation
5. Enhanced phosphorylation of p53 by ATM in response to DNA damage
6. Characterization of the tumor suppressor protein p53 as a protein kinase C substrate and a S100b-binding protein
7. Human p53 is phosphorylated by p60-cdc2 and cyclin B-cdc2
8. DNA damage induced p53 stabilization: No indication for an involvement of p53 phosphorylation
9. 32P ortho-phosphate in human fibroblasts
10. N-terminal processing: The methionine aminopeptidase and N alpha-acetyl transferase families
11. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53
12. A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
13. The double-stranded RNA activated protein kinase PKR physically associates with the tumor suppressor p53 protein and phosphorylates human p53 on serine 392 in vitro
14. p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest
15. Mutation of the serine 15 phosphorylation site of human p53 reduces the ability of p53 to inhibit cell cycle progression
16. Induction of nuclear accumulation of the tumor-suppressor protein p53 by DNA-damaging agents
17. Participation of the human p53 3'UTR in translational repression and activation following gamma-irradiation
18. Translational regulation of human p53 gene expression
19. p53 phosphorylation mutants retain transcription activity
20. One-dimensional SDS gel electrophoresis of proteins
21. The complexity of p53 modulation: Emerging patterns from divergent signals
22. Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain
23. Mdm2 promotes the rapid degradation of p53
24. JNK1, JNK2 and JNK3 are p53 N-terminal serine 34 kinases
25. p38 kinase mediates UV-induced phosphorylation of p53 protein at serine 389
26. Regulation of the specific DNA binding function of p53
27. Small peptides activate the latent sequence-specific DNA binding function of p53
28. Raf phosphorylates p53 in vitro and potentiates p53-dependent transcriptional transactivation in vivo
29. ATM associates with and phosphorylates p53: Mapping the region of interaction
30. p53 is phosphorylated in vitro and in vivo by the delta and epsilon isoforms of casein kinase 1 and enhances the level of casein kinase 1 delta in response to topoisomerase-directed drugs
31. p53 is phosphorylated by Cdk7-cyclin H in a p36mat1- dependent manner
32. Regulation of p53 stability by Mdm2
33. Phosphorylation of p53 serine 15 increases interaction with CBP
34. Human DNA-activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53
35. p53, the cellular gatekeeper for growth and division
36. p53 sites acetylated in vitro by Pcaf and p300 are acetylated in vivo in response to DNA damage
37. The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro
38. Ultraviolet radiation, but not gamma radiation or etoposide-induced DNA damage, results in the phosphorylation of the murine p53 protein at serine-389
39. Differential induction of transcriptionally active p53 following UV or ionizing radiation: Defects in chromosome instability syndromes?
40. Serine phosphorylation in the NH2 terminus of p53 facilitates transactivation
41. The p53 tumour suppressor protein is phosphorylated at serine 389 by casein kinase II
42. Phosphorylation of the p53 tumoursuppressor protein at three N-terminal sites by a novel casein kinase I-like enzyme
43. Phosphorylation of the tumor suppressor protein p53 by mitogen-activated protein kinases
44. Cyclins and cyclin-dependent kinases: Take your partners
45. Cdk2 kinase phosphorylates serine 315 of human p53 in vitro
46. Signaling to p53: Breaking the MDM2-p53 circuit
47. Stabilization of bovine trypsin by reductive methylation
48. DNA damage activates p53 through a phosphorylation-acetylation cascade
49. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels
50. DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2
51. DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel site, Ser20, requires tetramerization
52. DNA damage induces phosphorylation of the amino terminus of p53
53. Evidence for a second cell cycle block at G2/M by p53
54. p53 interacts with p34cdc2 in mammalian cells: Implications for cell cycle control and oncogenesis
55. A functional wild-type p53 gene is expressed in human acute myeloid leukemia cell lines
56. Regulation of the sequence-specific DNA binding function of p53 by protein kinase C and protein phosphatases
57. A role for ATR in the DNA damage-induced phosphorylation of p53
58. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
59. Critical role for Ser20 of human p53 in the negative regulation of p53 by Mdm2
60. Mutations in serines 15 and 20 of human p53 impair its apoptotic activity
61. Expression of a retinoic acid receptor gene in myeloid leukemia cells
62. Increased and altered DNA binding of human p53 by S and G2/M but not G1 cyclin-dependent kinases
63. ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 protiens
64. Elevated levels of wild-type p53 induced by radiolabeling of cells leads to apoptosis or sustained growth arrest