High-resolution cell division tracking demonstrates the Flt3-ligand- dependence of human marrow CD34+CD38- cell production in vitro

British Journal of Haematology

Volumn 98, Issue 3, 1997, Pages 528-539

  Nordon, Robert E ^a,b   Ginsberg, Steven S ^a   Eaves, Connie J ^a  

^a BRITISH COLUMBIA CANCER AGENCY   (Canada)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Cell division tracking; Flt3 ligand; In vitro expansion; Recruitment; Stem cells

Indexed keywords

GRANULOCYTE COLONY STIMULATING FACTOR; INTERLEUKIN 3; INTERLEUKIN 6; STEEL FACTOR;

ANTIGEN DETECTION; ARTICLE; BONE MARROW CELL; CELL CYCLE; CELL DIFFERENTIATION; CELL DIVISION; CELL PROLIFERATION; CELL SUBPOPULATION; CONTROLLED STUDY; DNA DETERMINATION; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; MITOSIS; PRIORITY JOURNAL;

EID: 0030883789     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.1997.2823097.x     Document Type: Article

References (32)

1. Ashley, D.M., Bol, S.J., Waugh, C. & Kannourakis, G. (1993) A novel approach to the measurement of different in vitro leukemic cell growth parameters: the use of PKH GL fluorescent probes. Leukemia Research, 17, 873-882.
2. Boehmer, R.M. (1979) Flow cytometric cell cycle analysis using the quenching of 33258 Hoechst fluorescence by bromodeoxyuridine incorporation. Cell and Tissue Kinetics, 12, 101-107.
3. + cells into the myeloid lineage. Blood, 80, 1405-1412.
4. Hodgkin, P.D., Lee, J-H. & Lyons, A.B. (1996) B cell differentiation and isotype switching is related to division cycle number. Journal of Experimental Medicine, 184, 277-281.
5. Hogge, D.E., Lansdorp, P.M., Reid, D., Gerhard, B. & Eaves, C.J. (1996) Enhanced detection, maintenance and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human Steel factor, interleukin-3 and granulocyte colony-stimulated factor. Blood, 88, 3765-3773.
6. - progenitor cells in long-term cultures and semisolid assays. Blood, 81, 2916-2924.
7. Jagers, P. (1975) Branching models in cell kinetics. Branching Processes with Biological Applications. John Wiley & Sons, London.
8. Kolber, M.A., Quinones, R.R., Gress, R.E. & Henkart, P.A. (1988) Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF). Journal of Immunological Methods, 108, 255-264.
9. Lange, W., Henschler, R. & Mertelsmann, R. (1996) Biological and clinical advances in stem cell expansion. Leukemia, 10, 943-945.
10. + cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow. Journal of Experimental Medicine, 175, 1501-1509.
11. Lansdorp, P.M. & Dragowska, W. (1993) Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors. Experimental Hematology, 21, 1321-1327.
12. + hemopoietic cells from human bone marrow. Journal of Experimental Medicine, 172, 363-366.
13. + bone marrow cells in relation to their colony formation. Blood, 81, 3211-3216.
14. Larochelle, A., Vormoor, J., Hanenberg, H., Wang, J.C.Y., Bhatia, M., Lapidot, T., Moritz, T., Murdoch, B., Xiao, X.L., Kato, I., Williams, D.A. & Dick, J.E. (1996) Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy. Nature Medicine, 2, 1329-1337.
15. Lyons, A.B. & Parish, C.R. (1994) Determination of lymphocyte division by flow cytometry. Journal of Immunological Methods, 171, 131-137.
16. Montgomery, D.C. (1991) Design and Analysis of Experiments. John Wiley & Sons, New York.
17. Muta, K., Krantz, S.B., Bondurant, M.C. & Wickrema, A. (1994) Distinct roles of erythropoietin, insulin-like growth factor I, and stem cell factor in the development of erythroid progenitor cells. Journal of Clinical Investigation, 94, 34-43.
18. Papayannopoulou, T., Brice, M., Farrer, D. & Kaushansky, K. (1996) Insights into the cellular mechanisms of erythropoietin-thrombopoietin synergy. Experimental Hematology, 24, 660-669.
19. Petzer, A.L., Hogge, D.E., Lansdorp, P.M., Reid, D.S. & Eaves, C.J. (1996a) Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium. Proceedings of the National Academy of Sciences of the United States of America, 93, 1470-1474.
20. -) human hematopoietic cells: novel responses to flt3-ligand and thrombopoietin. Journal of Experimental Medicine, 183, 2551-2558.
21. Riddell, S.R. & Greenberg, P.D. (1995) Cellular adoptive immunotherapy after bone marrow transplantation. Cancer Treatment Research, 76, 337-369.
22. Ross, S.M. (1970) Renewal theory. Applied Probability Models with Optimization Applications, p. 55. Dover Publications, New York.
23. + subpopulations of human bone marrow cells. Proceedings of the National Academy of Sciences of the United States of America, 91, 12223-12227.
24. - cells and maintains progenitor cells in vitro. Blood, 87, 3563-3570.
25. Sundstrom, C. & Nilsson, K. (1976) Establishment and characterization of human histiocytic lymphoma cell line (U-937). International Journal of Cancer, 17, 565-577.
26. Sutherland, H.J., Lansdorp, P.M., Henkelman, D.H., Eaves, A.C. & Eaves, C.J. (1990) Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proceedings of the National Academy of Sciences of the United States of America, 87, 3584-3588.
27. + bone marrow cells by c-kit-ligand, GM-CSF, and TNF alpha. Advances in Experimental Medicine and Biology, 378, 17-20.
28. - progenitor cells. Blood, 77, 1218-1227.
29. Verfaillie, C.M. & Miller, J.S. (1995) A novel single-cell proliferation assay shows that long-term culture-initiating cell (LTC-IC) maintenance over time results from the extensive proliferation of a small fraction of LTC-IC. Blood, 86, 2137-2145.
30. - primitive progenitor cells. Blood. 88, 1619-1631.
31. Young, J.C., Varma, A., DiGiusto, D. & Backer, M.P. (1996b) Retention of quiescent hematopoietic cells with high proliferative potential during ex vivo stem cell culture. Blood, 87, 545-556.
32. Zandstra, P.W., Conneally, E., Petzer, A.L., Piret, J.M. & Eaves, C.J. (1997) Cytokine manipulation of primitive human hematopoietic cell self-renewal. Proceedings of the National Academy of Sciences of the United States of America, 94, 4698-4703.