Active-site determination of a pyrimidine dimer glycosylase

Journal of Molecular Biology

Volumn 295, Issue 3, 2000, Pages 479-488

  Garvish, John F ^a,b   Lloyd, R Stephen ^b  

^a Department of Microbiology and Immunology ^*  (United States)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

Borohydride trapping; Chlorella virus pyrimidine dimer glycosylase (cv pdg); Cyclobutane pyrimidine dimer; DNA repair; Glycosylase

Indexed keywords

DNA GLYCOSYLTRANSFERASE; PYRIMIDINE DIMER; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIOPHAGE T4; CHLORELLA VIRUS; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

CHLORELLA VIRUS;

EID: 0034695420     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3369     Document Type: Article

References (19)

1. Evidence for an imino intermediate in the T4 endonuclease V reaction
2. Chlorella virus PBCV-1 encodes a homolog of the bacteriophage T4 UV damage repair gene denV
3. Role of active-site residues in the glycosylase step of T4 endonuclease V. Computer simulation studies on ionization states
4. The catalytic mechanism of a pyrimidine dimer-specific glycosylase (pdg)/abasic lyase, Chlorella virus-pdg
5. On-line procedures for alkylation of cysteine residues with 3-bromopropy-lamine prior to protein sequence analysis
6. Base excision repair of cyclobutane pyrimidine dimers
7. The initiation of DNA base excision repair of dipyrimidine photoproducts
8. Characterization of a novel cis-syn and trans-syn-II pyrimidine dimer glycosylase/AP lyase from a eukaryotic algal virus, Paramecium bursaria chlorella virus-1
9. Initiation of base excision repair: Glycosylase mechanisms and structures
10. DNA repair mechanisms for the recognition and removal of damaged DNA bases
11. X-ray structure of T4 endonuclease V: An excision repair enzyme specific for a pyrimidine dimer
12. Crystal structure of a pyrimidine dimer-specific excision repair enzyme from bacteriophage T4: Refinement at 1.45 Å X-ray analysis of the three active-site mutants
13. The critical active-site amine of the human 8-oxoguanine DNA glycosylase, hOgg1: Direct identification, ablation and chemical reconstitution
14. Reductive methylation of the amino terminus of endonuclease V eradicates catalytic activities. Evidence for an essential role of the amino terminus in the chemical mechanisms of catalysis
15. Preparation and characterization of a set of deoxyoligonucleotide 49-mers containing site-specific cis-syn, trans-syn-I, (6-4), and Dewar photoproducts of thymidylyl(3' → 5')-thymidine
16. Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure
17. Essential dynamics of DNA containing a cis.syn cyclobutane thymine dimer lesion
18. 2-terminal proline acts as a nucleophile in the glycosylase/AP-lyase reaction catalyzed by Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) protein