Effect of cytochalasin B on the induction of chromosome missegregation by colchicine at low concentrations in human lymphocytes

Mutagenesis

Volumn 14, Issue 1, 1999, Pages 43-49

  Minissi, Sandra ^a   Gustavino, Bianca ^a   Degrassi, Francesca ^b   Tanzarella, Caterina ^c   Rizzoni, Marco ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b CNR   (Italy)

^c ROMA TRE UNIVERSITY   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

COLCHICINE; CYTOCHALASIN B; PHYTOHEMAGGLUTININ;

ADULT; ANAPHASE; ARTICLE; CENTROMERE; CHROMATID; CHROMOSOME 11; CHROMOSOME 7; CHROMOSOME DAMAGE; CHROMOSOME LOSS; CHROMOSOME SEGREGATION; CONTROLLED STUDY; DNA PROBE; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOCYTE; MICRONUCLEUS; MITOSIS RATE; NORMAL HUMAN; POLYPLOIDY; PRIORITY JOURNAL; SISTER CHROMATID; TELOPHASE; TETRAPLOIDY;

ADULT; CELL DIVISION; CELLS, CULTURED; CHROMOSOME SEGREGATION; COLCHICINE; CYTOCHALASIN B; FEMALE; HUMANS; LYMPHOCYTES;

EID: 0032936727     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/14.1.43     Document Type: Article

References (21)

1. Antoccia, A., Tanzarella, C., Modesti, D. and Degrassi, F. (1993) Cytokinesis-blocked micronucleus assay with kinetochore detection in colchicine-treated human fibroblasts. Mutat. Res., 287, 93-99.
2. Carter, S.B. (1967) Effects of cytochalasins on mammalian cells. Nature, 21, 261-264.
3. Cimini, D., Tanzarella, C. and Degrassi F. (1997) Evaluation of chromosome malsegregation in anaphase and binucleated cells. In Proceedings of the AGI-SIMA 1st Joint Meeting, Orvieto (Italy), 23-26 September 1977.
4. Eastmond, D.A. and Pinkel, D. (1990) Detection of aneuploidy and aneuploidy-inducing agents in human lymphocyte using fluorescence in situ hybridization with chromosome-specific DNA probes. Mutat. Res., 234, 303-318.
5. Eastmond, D.A. and Tucker J.D. (1989) Identification of aneuploidy-inducing agents using cytokinesis-blocked human lymphocytes and an anti-kinetochore antibody. Environ. Mol. Mutagen., 13, 34-43.
6. Falck, G., Catalàn, J. and Norppa, H. (1997) Influence of culture time on the frequency and contents of human lymphocytes micronuclei with and without cytochalasin B. Mutat. Res., 392, 71-79.
7. Fenech, M. and Morley, A.A. (1985) Measurement of micronuclei in lymphocytes. Mutat. Res., 147, 29-36.
8. Gustavino, B., Degrassi, F., Filipponi, R., Modesti, D., Tanzarella, C. and Rizzoni, M. (1994) Mitotic non-disjunction in phytohemagglutinin stimulated human lymphocytes. Mutagenesis, 9, 17-21.
9. Heddle, J.A., Hite, M., Kirkhart, B., Mavournin, K., MacGregor, J.T., Newell, G.W. and Salamone, M.F. (1983) The induction of micronuclei as a measure of genotoxicity. Mutat. Res., 123, 61-118.
10. Lindholm, C., Norppa, H., Hayashi, M. and Sorsa, M. (1991) Induction of micronuclei and anaphase aberrations by cytochalasin B in human lymphocyte cultures. Mutat. Res., 260, 369-375.
11. Marshall, R.R., Murphy, M., Kirkland, D.J. and Bentley, K.S. (1996) Fluorescence in situ hybridisation with chromosome-specific centromeric probes: a sensitive method to detect aneuploidy. Mutat. Res., 372, 233-245.
12. Migliore, L., Nieri, M., Amodio, S., Loprieno N. (1989) The human lymphocytes micronucleus assay: a comparison between whole-blood and separated-lymphocytes culture. Mutat. Res., 227, 167-172.
13. 2 metaphases. Atti Ass. Genet. Ital. Vol. XLII, Riccione (RN), 2-5 October.
14. Norppa, H., Renzi, L. and Lindholm, C. (1993) Detection of whole chromosomes in micronuclei of cytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridisation. Mutagenesis, 8, 519-525.
15. Perry, P. and Wolff, S. (1974) New Giemsa method for the differential staining of sister chromatids. Nature, 251, 156-158.
16. Prosser, J.S., Moquet, J.E., Lloyd, D.C. and Edwards, A.A. (1988) Radiation induction of micronuclei in human lymphocytes. Mutat. Res., 199, 37-45.
17. Sgura, A., Antoccia, A., Ramirez., M.J., Marcos, R., Tanzarella, C., Degrassi F. (1997) Micronuclei, centromere-positive micronuclei and chromosome nondjunction in cytokinesis blocked human lymphocytes following mitomycin C and vincristine treatment. Mutat. Res., 392, 97-107.
18. Surrallés, J., Falck, G. and Norppa, H. (1996) In vivo cytogenetic damage revealed by FISH analysis of micronuclei in uncultured human T lymphocytes. Cytogenet. Cell Genet., 75, 151-154.
19. Zijno, A., Marcon, F., Leopardi, P. and Crebelli, R. (1994) Simultaneous detection of X-chromosome loss and non-disjunction in cytokinesis-blocked human lymphocytes by in situ hybridization with centromeric DNA probe: implications for the human lymphocyte in vitro micronucleus assay using cytochalasin B. Mutagenesis, 9, 225-232.
20. Zijno, A., Marcon, F., Leopardi, P. and Crebelli, R (1996a) Analysis of chromosome segregation in cytokinesis-blocked human lymphocytes: non-disjunction is the prevalent damage resulting from low concentration exposure to spindle poisons. Mutagenesis, 11, 335-340.
21. Zijno, A., Leopardi, P., Marcon, F. and Crebelli, R (1996b) Analysis of chromosome segregation by means of fluorescence in situ hybridization: application to cytokinesis-blocked human lymphocytes. Mutat. Res., 372, 211-219.