The docking stage of yeast vacuole fusion requires the transfer of proteins from a cis-SNARE complex to a Rab/Ypt protein

Journal of Cell Biology

Volumn 148, Issue 6, 2000, Pages 1231-1238

  Price, Albert ^a,b   Seals, Darren ^a   Wickner, William ^a   Ungermann, Christian ^a,c   Wickner, Bill ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Priming; Rab Ypt effector; Vps39 Vam6p; Vps41 Vam2p; Ypt7p

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN YPT7P; SNARE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL VACUOLE; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE;

ADENOSINE TRIPHOSPHATASES; CARRIER PROTEINS; FUNGAL PROTEINS; MEMBRANE FUSION; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; NUCLEAR PROTEINS; RAB GTP-BINDING PROTEINS; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SNARE PROTEINS; VACUOLES; VESICULAR TRANSPORT PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0034689024     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.148.6.1231     Document Type: Article

References (45)

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2. Sec15 protein, an essential component of the exocytotic apparatus, is associated with the plasma membrane and with a soluble 19.5S particle
3. Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis
4. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis
5. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins
6. Sec1p binds to SNARE complexes and concentrates at sites of secretion
7. The Rab5 effector EEA1 is a core component of endosome docking
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9. rbSec1A and B colocalize with syntaxin and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin
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11. The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis
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14. The N-ethylmaleimide-sensitive fusion protein and α-SNAP induce a conformational change in syntaxin
15. Genetic and morphological analyses reveal a critical interaction between the C-termini of two SNARE proteins and a parallel four helical arrangement for the exocytic SNARE complex
16. Subunit structure of the mammalian exocyst complex
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28. Proteins needed for vesicle budding from the Golgi are also required for the docking step of homotypic vacuole fusion
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