Proteins needed for vesicle budding from the Golgi complex are also required for the docking step of homotypic vacuole fusion

Journal of Cell Biology

Volumn 148, Issue 6, 2000, Pages 1223-1229

  Price, Albert ^a,b   Wickner, William ^a   Ungermann, Christian ^a,c  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

SNAP Sec17p; NSF Sec18p; Priming; Vps39 Vam6p; Vps41 Vam2p

Indexed keywords

SNARE PROTEIN; SNARE PROTEIN VAM2P; SNARE PROTEIN VAM6P; UNCLASSIFIED DRUG;

ARTICLE; CELL VACUOLE; GOLGI COMPLEX; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

CARRIER PROTEINS; FUNGAL PROTEINS; GOLGI APPARATUS; KINETICS; MEMBRANE FUSION; MEMBRANE PROTEINS; NUCLEAR PROTEINS; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SNARE PROTEINS; VACUOLES; VESICULAR TRANSPORT PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0034689021     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.148.6.1223     Document Type: Article

References (37)

1. Coupled ER to Golgi transport reconstituted with purified cytosolic proteins
2. Syntaxin and synaptobrevin function downstream of vesicle docking in Drosophila
3. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins
4. Novel Golgi to vacuole delivery pathway in yeast: Identification of a sorting determinant and required transport component
5. A quantitative assay to measure homotypic vacuole fusion in vitro
6. Homotypic vacuole fusion requires Sec17p (yeast α-SNAP) and Sec18p (yeast NSF)
7. The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance
8. Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway
9. SNAREs and NSF in targeted membrane fusion
10. Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly
11. Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF)
12. Sec18p (NSF)-driven release of Sec17p (α-SNAP) can precede docking and fusion of yeast vacuoles
13. Phosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion
14. Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13
15. Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membranes and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae
16. Homotypic vacuolar fusion mediated by t- and v-SNAREs
17. The diversity of Rab proteins in vesicle transport
18. Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin, and SNAP-25 in the membrane of synaptic vesicles
19. 2+/calmodulin signals the completion of docking and triggers a late step of vacuole fusion
20. Control of the terminal step of intracellular membrane fusion by protein phosphatase 1
21. Transport vesicle docking: SNAREs and associates
22. Characterization of VPS41, a gene required for vacuolar trafficking and high-affinity iron transport in yeast
23. Biogenesis of the vacuole in Saccharomyces cerevisiae
24. Formation of AP-3 transport intermediates requires Vps41p function
25. Mechanisms of intracellular protein transport
26. Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs
27. The exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae
28. Vam7p, a vacuolar SNAP-25 homolog, is required for SNARE complex integrity and vacuole docking and fusion
29. Defining the functions of trans-SNARE pairs
30. Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic vacuole fusion
31. Vacuole acidification is required for trans-SNARE pairing. LMA1 release, and homotypic fusion
32. Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. I. Isolation and characterization of two classes of vam mutants
33. 2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance
34. LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion
35. Isolation of yeast mutants defective for localization of vacuolar vital dyes