Vam7p, a vacuolar SNAP-25 homolog, is required for SNARE complex integrity and vacuole docking and fusion

EMBO Journal

Volumn 17, Issue 12, 1998, Pages 3269-3276

  Ungermann, Christian ^a   Wickner, William ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

SNAP 25; SNARE complex; Vacuole fusion; Vam7p

Indexed keywords

CELL MEMBRANE PROTEIN; MUTANT PROTEIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25;

ARTICLE; CELL MEMBRANE; CELL VACUOLE; COMPLEX FORMATION; CONTROLLED STUDY; HYDROPHOBICITY; MEMBRANE FUSION; MORPHOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SEQUENCE HOMOLOGY;

ADENOSINE TRIPHOSPHATASES; FUNGAL PROTEINS; MEMBRANE PROTEINS; NERVE TISSUE PROTEINS; QC-SNARE PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SNARE PROTEINS; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; VACUOLES; VESICULAR TRANSPORT PROTEINS;

EID: 0032526955     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.12.3269     Document Type: Article

References (60)

1. Ausubel, F., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (eds) (1995) Short Protocols in Molecular Biology. 3rd edn. John Wiley & Sons, Inc., New York.
2. 2+-dependent exocytosis. J. Biol. Chem., 271, 20227-20230.
3. Blasi, J., Chapman, E.R., Link, E., Binz, T., Yamasaki, S., DeCamilli, P., Südhof, T.C., Niemann, H. and Jahn, R. (1993) Botulinum neurotoxin A selectively cleaves the synaptic protein SNAP-25. Nature, 365, 160-163.
4. Brennwald, P., Kearns, B., Champion, K., Keränen, S., Bankaitis, V. and Novick, P. (1994) Sec9 is a SNAP-25 like component of the yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell, 79, 245-258.
5. Cao, X., Ballew, N. and Barlowe, C. (1998) Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J., 17, 2156-2165.
6. Chapman, E.R., An, S., Barton, N. and Jahn, R. (1994) SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils. J. Biol. Chem., 269, 27427-27432.
7. Conradt, B., Shaw, J., Vida, T., Emr, S. and Wickner, W. (1992) In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae. J. Cell Biol., 119, 1469-1479.
8. Couves, A. and Gerst, J.E. (1994) Yeast Snc proteins complex with Sec9. J. Biol. Chem., 269, 23391-23394.
9. Fasshauer, D., Bruns, D., Shen, B., Jahn, R. and Brünger, A.T. (1997a) A structural change occurs upon binding of syntaxin to SNAP-25. J. Biol. Chem., 272, 4582-4590.
10. Fasshauer, D., Henning, O., Eliason, W.K., Jahn, R. and Brünger, A.T. (1997b) Structural changes are associated with soluble N-ethylmleimide-sensitive fusion protein attachment protein receptor complex formation. J. Biol. Chem., 272, 28036-28041.
11. Ferro-Novick, S. and Jahn, R. (1994) Vesicle fusion from yeast to man. Nature, 370, 191-193.
12. Fischer von Mollard, G., Notwehr, S. and Stevens, T. (1997) The yeast v-SNARE Vtilp mediates two vesicle transport pathways through interaction with the t-SNAREs Sed5p and Pep12p. J. Cell Biol., 137, 1511-1524.
13. 2+-dependent exocytosis in chromaffin cells. FEBS Lett., 372, 39-42.
14. Haas, A. (1995) A quantitative assay to measure homotypic vacuole fusion in vitro. Methods Cell Sci., 17, 283-294.
15. Haas, A. and Wickner, W. (1996) Homotypic vacuolar fusion requires Sec17p (yeast α-SNAP) and Sec18p (yeast NSF). EMBO J., 15, 3296-3305.
16. Haas, A., Conradt, B. and Wickner, W. (1994) G-protein ligands inhibit in vitro reactions of vacuole inheritance. J. Cell Biol., 126, 87-97.
17. Haas, A., Scheglmann, D., Lazar, T., Gallwitz, D. and Wickner, W. (1995) The GTPase Ypt7p is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. EMBO J., 14, 5258-5270.
18. Harlow, E. and Lane, O. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY.
19. Hata, Y., Slaughter, C.A. and Südhof, T.C. (1993) Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin. Nature. 366, 347-351.
20. Hay, J.C. and Scheller, R. (1997) SNAREs and NSF in targeted membrane fusion Curr. Opin. Cell Biol., 9, 505-512.
21. Hay, J.C., Chao, D.S., Kuo, C. and Scheller, R.H. (1997) Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells. Cell, 89, 149-158.
22. Hayashi, T., McMahon, H., Yamasaki, S., Binz, T., Hata, Y., Südhof, T.C. and Niemann, H. (1994) Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. EMBO J., 13, 5051-5061.
23. Hayashi, T., Yamasaki, S., Nauenburg, S., Binz, T. and Niemann, H. (1995) Disassembly of the reconstituted synaptic vesicle membrane fusion complex in vitro. EMBO J., 14, 2317-2325.
24. Holthuis, J.C.M., Nichols, B.J., Dhruvakumar, S. and Pelham, H.R.B. (1998) Two syntaxin homologues in the TGN/endosomal system in yeast. EMBO J., 17, 113-126.
25. Lian, J.P. and Ferro-Novick, S. (1993) Bos1p, an integral membrane protein of the ER to Golgi transport vesicle, is required for their fusion competence. Cell, 73, 735-745.
26. Lupashin, V.V. and Waters, M.G. (1997) t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase. Science, 276, 1255-1258.
27. Lupashin, V.V., Pokrovskaya, I.D., McNew, J.A., Waters, M.G. (1997) Characterization of a novel yeast SNARE protein implicated in Golgi retrograde traffic. Mol. Biol. Cell, 8, 2659-2676.
28. Martin, T.F.J. (1997) Stages of regulated exocytosis. Trends Cell Biol., 7, 271-276.
29. Mayer, A. and Wickner, W. (1997) Docking of yeast vacuoles is catalyzed by the ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF). J. Cell Biol., 136, 307-317.
30. Mayer, A., Wickner, W. and Haas, A. (1996) Sec18p (NSF)-driven release of Sec17p (α-SNAP) precedes docking and fusion of yeast vacuoles. Cell, 85, 83-94.
31. McMahon, H.T. and Sudhof, T.C. (1995) Synaptic core complex of synaptobrevin, syntaxin, and SNAP-25 forms high affinity alpha-SNAP binding site. J. Biol. Chem., 270, 2213-2217.
32. 2+-dependent triggering of neurotransmitter exocytosis. Proc. Natl Acad. Sci. USA, 93, 10471-10476.
33. Neiman, A.M. (1998) Prospore membrane formation defines a developmentally regulated branch of the secretory pathway in yeast. J. Cell Biol., 140, 29-37.
34. Newman, A.P. Groesch, M. and Ferro-Novick, S. (1992) Bos1p, a membrane protein required for ER to Golgi transport in yeast, co-purifies with the carrier vesicles and with Bet1p and the ER membrane. EMBO J., 12, 3609-3617.
35. Nichols, B.J., Ungermann, C., Pelham, H.R.B., Wickner, W. and Haas, A. (1997) Homotypic vacuolar fusion mediated by v- and t-SNAREs. Nature, 387, 199-202.
36. Novick, P. and Zerial, M. (1997) The diversity of rab proteins in vesicle transport. Curr. Opin. Cell Biol., 9, 496-504.
37. Otto, H., Hanson, P.I. and Jahn, R. (1997) Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin and SNAP-25 in the membrane of synaptic vesicles. Proc. Natl Acad. Sci. USA, 94, 6197-6201.
38. Pevsner, J., Hsu, S.-C, Braun, J.E.A., Calakos, N., Ting, A.E., Bennett, M.K. and Scheller, R.H. (1994) Specificity and regulation of a synaptic vesicle docking complex. Neuron, 13, 353-361.
39. Pfeffer, S.R. (1996) Transport vesicle docking: SNARE and associates. Annu. Rev. Cell Biol. Dev. Biol., 12, 441-461.
40. Rice, L.M., Brennwald, P. and Brünger, A.T. (1997) Formation of a yeast SNARE complex is accompanied by significant structural changes. FEBS Lett., 415, 49-55.
41. Rossi, G., Salminen, A., Rice, L.M., Brünger, A.T. and Brennwald, P. (1997) Analysis of a yeast SNARE complex reveals remarkable similarity to the neuronal SNARE complex and a novel function for the C terminus of the SNAP-25 homolog. Sec9. J. Biol. Chem., 272, 16610-16617.
42. Rothman, J.E. (1994) Mechanisms of intracellular membrane fusion. Nature, 372, 55-63.
43. Søgaard, M., Tani, K., Ye, R.R., Geromanos, S., Tempst, P., Kirchhausen, T., Rothman, J.E. and Söllner, T. (1994) A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell, 78, 937-48.
44. Söllner, T., Whiteheart, S.W., Brunner, M., Erdjument-Bromage, H., Geromanos, S., Tempst, P. and Rothman, J.E. (1993a) SNAP receptors implicated in vesicle targeting and fusion. Nature, 362, 318-324.
45. Söllner, T., Bennett, M.K., Whiteheart, S., Scheller, R.H. and Rothman, J.E. (1993b) A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation and fusion. Cell, 75, 409-418.
46. Stone, S., Sacher, M., Mao, Y., Carr, C., Lyons, P., Quinn, A.M. and Ferro-Novick, S. (1997) Bet1p activates the v-SNARE Bos1p. Mol. Biol. Cell, 8, 1175-1181.
47. Südhof, T.C. (1995) The synaptic vesicle cascade: a cascade of protein-protein interactions. Nature, 342, 645-653.
48. Tagaya, M., Toyonaga, S., Takahashi, M., Yamamoto, A., Fujiwara, T., Akagawa, K., Moriyama, Y. and Mizushima, S. (1995) Syntaxin 1 (HPC-1) is associated with chromaffin granules. J. Biol. Chem., 270, 15930-15933
49. Tagaya, M., Genma, T., Yamamoto, A., Kozaki, S. and Mizushima, S. (1996) SNAP-25 is present on chromaffin granules and acts as a SNAP receptor. FEBS Lett., 394, 83-86.
50. Ungermann, C., Nichols, B.J., Pelham, H.R.B. and Wickner, W. (1998) A vacuolar v-t-SNARE complex, the predominant form in vivo and on isolated organelles, is disassembled and activated for docking and fusion. J. Cell Biol., 140, 61-69.
51. Veit, M., Söllner, T.H. and Rothman, J.E. (1996) Multiple palmitoylation of synaptotagmin and the t-SNARE SNAP-25. FEBS Lett., 385, 119-123.
52. Wada, Y. and Anraku, Y. (1992) Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. VAM7, a gene for regulating morphogenic assembly of the vacuoles. J. Biol. Chem., 267, 18671-18675.
53. Wada, Y. Ohsumi, Y. and Anraku, Y. (1992) Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. Isolation and characterization of two classes of vam mutants. J. Biol. Chem., 267, 18665-18670.
54. Wada, Y. Nakamura, N., Ohsumi, Y. and Hirata, A. (1997) Vam3p, a new member of syntaxin related proteins, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae. J. Cell Sci., 110, 1299-1306.
55. Walch-Solimena, C., Blasi, J., Edelmann, L., Chapman, E.R., Fischer von Mollard, G. and Jahn, R. (1995) The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling. J. Cell Biol., 128, 637-45.
56. Weimbs, T., Low, S.H., Chapin, S.J., Mostov, K.E., Bucher, P. and Hofmann, K. (1997) A conserved domain is present in different families of vesicular fusion proteins: a new superfamily. Proc. Natl Acad. Sci., 94, 3046-3051.
57. Xu, Z. and Wickner, W. (1996) Thioredoxin is required for vacuole inheritance in S.cerevisiae. J. Cell Biol., 132, 787-794.
58. 2 cooperates with Sec18p(NSF) to promote yeast vacuole inheritance. J. Cell Biol., 136, 299-306.
59. Xu, Z., Sato, K. and Wickner, W. (1998) LMA1 binds to vacuoles via Sec18p (NSF), transfers after ATP-hydrolysis to a complex with the t-SNARE (Vam3p), and is released during organelle fusion. Cell, in press.
60. Zhang, T., Wong, S.H., Tang, B.L., Xu, Y. Peter, F., Subramaniam, N. and Hong, W. (1997) The mammalian protein (rbet1) homologous to yeast Betlp is primarily associated with the pre-Golgi intermediate compartment and is involved in vesicular transport from the endoplasmic reticulum to the Golgi apparatus. J. Cell Biol., 139, 1157-1168.