An approach for obtaining perfect hybridization probes for unknown polyketide synthase genes: A search for the epothilone gene cluster

Gene

Volumn 247, Issue 1-2, 2000, Pages 97-102

  Santi, Daniel V ^a   Siani, Michael A ^a   Julien, Bryan ^a   Kupfer, Doris ^b   Roe, Bruce ^b  

^a KOSAN BIOSCIENCES INC   (United States)

^b UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

Ketide; Myxobacteria; Non ribosomal peptide; Sorangium cellulosum

Indexed keywords

POLYKETIDE SYNTHASE;

ARTICLE; BACILLUS SUBTILIS; COMPUTER SIMULATION; DNA LIBRARY; DNA SEQUENCE; GENE CLUSTER; GENETIC ANALYSIS; HYBRIDIZATION; PRIORITY JOURNAL; STREPTOMYCES; TECHNIQUE;

ANTINEOPLASTIC AGENTS; BACILLUS SUBTILIS; COMPUTER SIMULATION; DNA PROBES; DNA, BACTERIAL; EPOTHILONES; EPOXY COMPOUNDS; GENOME, BACTERIAL; MULTIENZYME COMPLEXES; MULTIGENE FAMILY; MYXOCOCCALES; NUCLEIC ACID HYBRIDIZATION; PEPTIDE SYNTHASES; SEQUENCE ANALYSIS, DNA; THIAZOLES;

BACILLUS SUBTILIS; MYXOCOCCALES; POLYANGIUM CELLULOSUM; SORANGIUM; STREPTOMYCES; UNIDENTIFIED BACTERIOPHAGE;

EID: 0034682033     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00113-X     Document Type: Article

References (22)

1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
2. Arrowsmith T., Malpartida F., Sherman D., Birch A., Hopwood D., Robinson J. Characterisation of actI-homologous DNA encoding polyketide synthase genes from the monensin producer Streptomyces cinnamonensis. Mol. Gen. Genet. 234:1992;254-264.
3. Bodenteich A., Chissoe S., Wang Y.F., Roe B.A. Shotgun cloning as the strategy of choice to generate templates for high throughput dideoxynucleotide sequencing. Adams M.D., Fields C., Venter C. Automated DNA Sequencing and Analysis Techniques. 1994;42-50 Academic Press, London.
4. Cane E.D. Polyketide and Nonribosomal Polypeptide Biosynthesis, Chemical Reviews. CHREAY. 1997;2463-2705.
5. Cortés J., Haydock S.F., Roberts G.A., Bevitt D.J., Leadlay P.F. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea. Nature. 348:1990;176-178.
6. Donadio S., Staver M., McAlpine J., Swanson S., Katz L. Modular organization of genes required for complex polyketide biosynthesis. Science. 252:1991;675-679.
7. Hopwood D.A. Genetic contributitions to understanding polyketide synthases. Chem. Rev. 97:1997;2465-2497.
8. Jaoua S., Neff S., Schupp T. Transfer of mobilizable plasmids to Sorangium cellulosum and evidence for their integration into the chromosome. Plasmid. 28:1992;157-165.
9. Julien B., Shah S., Ziermann R., Goldman R., Katz L., Khosla C. Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum. Gene. 2000;. in press.
10. Kunst F., et al. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 390:1997;249-256.
11. Lander E.S., Waterman M.S. Genomic mapping by fingerprinting random clones: a mathematical analysis. Genomics. 2:1988;231-239.
12. Ligon J.M., Schupp T., Beck J.J., Hill D.S., Neff S., Ryals J.A. Genes for the Biosynthesis of Soraphen. 1998;Novartis Finance Corporation, USA.
13. Marahiel A.M., Stachelhaus T., Mootz D.H. Modular peptide synthetases involved in nonribosomal peptide synthesis. Chem. Rev. 97:1997;2651-2673.
14. Marahiel M.A. Protein templates for the biosynthesis of peptide antibiotics. Chem. Biol. 4:1997;561-567.
15. Proctor R.H., Desjardins A.E., Plattner R.D., Hohn T.M. A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal Genet. Biol. 27:1999;100-112.
16. Roe B.A. University of Oklahoma's Advanced Center for Genome Technology ACGT. 1999;. http://www.genome.ou.edu.
17. Ruan X., Stassi D., Lax S., Katz L. A second type-I PKS gene cluster isolated from Streptomyces hygroscopicus ATCC 29253, a rapamycin-producing strain. Gene. 203:1997;1-9.
18. Schupp T., Toupet C., Cluzel B., Neff S., Hill S., Beck J., Ligon J.M. A Sorangium cellulosum (Myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A: cloning, characterization and homology to polyketide synthase genes from actinomycetes. J. Bacteriol. 177:1995;3673-3679.
19. Steller S., Vollenbroich D., Leenders F., Stein T., Conrad B., Hofemeister J., Jacques P., Thonart P., Vater J. Structural and functional organization of the fengycin synthetase multienzyme system from Bacillus subtilis b213 and A1/3. Chem. Biol. 6:1999;31-41.
20. Tang L., Shah S., Chung L., Carney J., Katz L., Khosla C., Julien B. Cloning and Heterologous Expression of the Epothilone Gene Cluster. 2000.
21. Tsuge K., Ano T., Hirai M., Nakamura Y., Shoda M. The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production. Antimicrob. Agents Chemother. 43:1999;2183-2192.
22. Wall L., Christiansen T., Schwartz R.I. Programming Perl. 1991;O'Reilly, Sebastopol.