Site-directed mutagenesis of the regulatory domain of Escherichia coli carbamoyl phosphate synthetase identifies crucial residues for allosteric regulation and for transduction of the regulatory signals

Journal of Molecular Biology

Volumn 299, Issue 4, 2000, Pages 979-991

  Fresquet, Vicente ^a   Mora, Paz ^a   Rochera, Lourdes ^a   Ramón Maiques, Santiago ^b   Rubio, Vicente ^b   Cervera, Javier ^a  

^a CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

^b INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

Author keywords

Allosteric regulation; Carbamoyl phosphate synthetase; Nucleotide effectors; Pyrimidine biosynthesis; Site directed mutagenesis

Indexed keywords

ARGININE; BACTERIAL ENZYME; CARBAMOYL PHOSPHATE; CARBAMOYL PHOSPHATE SYNTHASE; GLUTAMINE; INOSINE PHOSPHATE; ORNITHINE; PYRIMIDINE DERIVATIVE; URIDINE PHOSPHATE;

ALLOSTERISM; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN DOMAIN; PYRIMIDINE SYNTHESIS; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; ULTRACENTRIFUGATION;

ESCHERICHIA COLI;

EID: 0034674163     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3794     Document Type: Article

References (44)

1. Binding of N-acetyl-L-glutamate to rat liver carbamoyl phosphate synthetase (ammonia)
2. Affinity cleavage of carbamoyl-phosphate synthetase I localizes regions of the enzyme interacting with the molecule of ATP that phosphorylates carbamate
3. Oxidative inactivation of carbamoyl phosphate synthetase (ammonia). Mechanism and sites of oxidation, degradation of the oxidized enzyme, and inactivation by glycerol, EDTA, and thiol protecting agents
4. Binding of allosteric affectors to carbamyl-phosphate synthetase from Escherichia coli
5. Conversion of UMP, an allosteric inhibitor of carbamyl phosphate synthetase, to an activator by modification of the UMP ribose moiety
6. Regulation of Escherichia coli carbamoyl phosphate synthetase. Evidence for overlap of the allosteric nucleotide binding sites
7. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
8. Allosteric effects of carbamoyl phosphate synthetase from Escherichia coli are entropydriven
9. Location of the binding site for the allosteric activator IMP in the COOH-terminal domain of Escherichia coli carbamyl phosphate synthetase
10. Photoaffinity labelling with the activator IMP and site-directed mutagenesis of histidine 995 of carbamoyl phosphate synthetase from Escherichia coli demonstrate that the binding site for IMP overlaps with that for the inhibitor UMP
11. Phosphorylation and activation of hamster carbamyl phosphate synthetase II by c-AMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis
12. The influence of effectors and subunit interactions on Escherichia coli carbamyl phosphate synthetase studied by differential scanning calorimetry
13. Photoaffinity labelling with UMP of lysine 992 of carbamyl phosphate synthetase from Escherichia coli allows identification of the binding site for the pyrimidine inhibitor
14. - by ATP in the carboxylation of biotin
15. Regulatory changes in the control of carbamoyl phosphate synthetase induced by truncation and mutagenesis of the allosteric binding domain
16. Serine 948 and threonine 1042 are crucial residues for allosteric regulation of Escherichia coli carbamoylphosphate synthetase and illustrate coupling effects of activation and inhibition pathways
17. Mutational analysis of carbamyl phosphate synthetase. Substitution of Glu841 leads to loss of functional coupling between the two catalytic domains of the synthetase subunit
18. Function of the major synthetase subdomains of carbamyl-phosphate synthetase
19. Protein-ligand binding studies with a table-top, air-driven, high-speed centrifuge
20. Improved methods for building protein models in electron density maps and the location of errors in these models
21. Rapid an efficient site-specific mutagenesis without phenotypic selection
22. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
23. Identification of the regulatory domain of the mammalian multi-functional protein CAD by the construction of an Escherichia coli hamster hybrid carbamyl-phosphate synthetase
24. Mechanism and regulation of the glutamine-dependent carbamyl phosphate synthetase of Escherichia coli
25. Raster3D version 2. 0. A program for photorealistic molecular graphics
26. Localization of the site for the nucleotide effectors of Escherichia coli carbamoyl phosphate synthetase using site-directed mutagenesis
27. The carB gene of Escherichia coli: A duplicated gene coding for the large subunit of carbamyl-phosphate synthetase
28. Reversible binding of Pi by beef heart mitochondrial adenosine triphosphatase
29. A rapid colorimetric assay for carbamyl phosphate synthetase I
30. Dissection of the functional domains of Escherichia coli carbamoyl phosphate synthetase by site-directed mutagenesis
31. Linkage between self association and catalytic activity of Escherichia coli carbamyl phosphate synthetase
32. Physical location of the site for N-acetyl-L-glutamate, the allosteric activator of carbamoyl phosphate synthetase, in the 20-kilodalton COOH-terminal domain
33. In vivo synthesis of carbamyl phosphate from NH3 by the large subunit of Escherichia coli carbamyl phosphate synthetase
34. Domain structure of the large subunit of Escherichia coli carbamoyl phosphate synthetase. Location of the binding site for the allosteric inhibitor UMP in the COOH-terminal domain
35. Mechanism of carbamoyl phosphate synthetase from Escherichia coli. Binding of the ATP molecules used in the reaction and sequestration by the enzyme of the ATP molecule that yields carbamoyl phosphate
36. DNA sequencing with chain-terminating inhibitors
37. Reduced surface: An efficient way to compute molecule surfaces
38. Mammalian carbamyl phosphate synthetase (CPS). DNA sequence and evolution of the CPS domain of the Syrian hamster multifunctional protein CAD
39. Structure of carbamoyl phosphate synthetase: A journey of 96 Å from substrate to product
40. The structure of carbamoyl phosphate synthetase determined to 2.1 Å resolution
41. The binding of inosine monophosphate to Escherichia coli carbamoyl phosphate synthetase
42. Three-dimensional structure of the biotin carboxylase subunit of acetyl-CoA carboxylase