Photoaffinity labeling with the activator IMP and site-directed mutagenesis of histidine 995 of carbamoyl phosphate synthetase from Escherichia coli demonstrate that the binding site for IMP overlaps with that for the inhibitor UMP

Biochemistry

Volumn 38, Issue 13, 1999, Pages 3910-3917

  Bueso, Jorge ^a   Cervera, Javier ^a   Fresquet, Vicente ^a   Marina, Alberto ^a,b   Lusty, Carol J ^c   Rubio, Vicente ^a,b  

^a CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

^b INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

^c PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAMOYL PHOSPHATE SYNTHASE; INOSINE PHOSPHATE; URIDINE PHOSPHATE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CROSS LINKING; ENZYME ACTIVITY; ENZYME BINDING; ENZYME CONFORMATION; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

EID: 0033616696     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi982871f     Document Type: Article

References (25)

1. Anderson, P. M., and Meister, A. (1966) Biochemistry 5, 3164-3169.
2. Pierard, A. (1966) Science 154, 1572-1577.
3. Meister, A. (1989) Adv. Enzymol. Relat. Areas Mol. Biol. 62, 315-374.
4. Nyunoya, H., and Lusty, C. J. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 4629-4633.
5. Rubio, V., Cervera, J., Lusty, C. J., Bendala, E., and Britton, H. G. (1991) Biochemistry 30, 1068-1075.
6. Thoden, J. B., Holden, H. M., Wesenberg, G., Raushel, F. M., and Rayment, I. (1997) Biochemistry 36, 6305-6316.
7. Bueso, J., Lusty, C. J., and Rubio, V. (1994) Biochem. Biophys. Res. Commun. 203, 1083-1089.
8. Rodríguez-Aparicio, L. B., Guadalajara, A. M., and Rubio, V. (1989) Biochemistry 28, 3070-3074.
9. Liu, X., Guy, H. I., and Evans, D. R. (1994) J. Biol. Chem. 269, 27747-27755.
10. Boettcher, B., and Meister, A. (1981) J. Biol. Chem. 256, 5977-5980.
11. Boettcher, B., and Meister, A. (1982) J. Biol. Chem. 257, 13971-13976.
12. Braxton, B. L., Mullins, L. S., Raushel, F. M., and Reinhart, G. D. (1992) Biochemistry 31, 2309-2316.
13. Cervera, J., Bendala. E., Britton, H. G., Bueso, J., Nassif, Z., Lusty, C. J., and Rubio, V. (1996) Biochemistry 35, 7247-7255.
14. Cerwinski, R. M., Mareya, S. M., and Raushel, F. M. (1995) Biochemistry 34, 13920-13927.
15. Henderson, L. E., Oroszlan, S., and Konigsberg, W. (1979) Anal. Biochem. 93, 153-157.
16. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Strühl, K. (1997) Current protocols in molecular biology, Wiley-Interscience, New York.
17. Guillou, F., Liao, H., García-España, A., and Lusty, C. J. (1992) Biochemistry'31, 1656-1664.
18. Kunkel, T. A., Roberts, J. D., and Zakour, R. A. (1987) Methods Enzymol. 154, 367-382.
19. Sanger, F., Nieklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467.
20. Rubio, V., and Grisolia, S. (1977) Biochemistry 16, 321-329.
21. Belew, M., Fohlman, J., and Janson, J. C. (1978) FEBS Lett 91, 302-304.
22. Schott, H. N., and Shetlar, M. D. (1974) Biochem. Biophys. Res. Commun. 59, 1112-1116.
23. Anderson, P. M. (1977) Biochemistry 16, 587-593.
24. Abdelal, A. T. H., and Ingraham, J. L. (1975) J. Biol. Chem. 250, 4410-4417.
25. Evans, D., and Guy, H. (1997) Paths to Pyrimidines 5, 1 -10.