Two-wavelength fluorescence assay for DNA repair

Analytical Biochemistry

Volumn 287, Issue 2, 2000, Pages 313-318

  Roguev, Assen ^a   Russev, George ^a  

^a INSTITUTE OF MOLECULAR BIOLOGY   (Bulgaria)

Author keywords

DNA repair; Green fluorescent proteins; HEK293 cells; Host cell reactivation assay

Indexed keywords

CYTOLOGY; DNA; FLUORESCENCE; GENES; RED SHIFT;

DNA REPAIR; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HEK293 CELLS; HOST CELLS; QUANTITATIVE ASSAY; TRANSFECTION EFFICIENCY;

PROTEINS;

DNA; ENHANCED GREEN FLUORESCENT PROTEIN; ENHANCED YELLOW FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; NUCLEOTIDE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA REPAIR; EMBRYO; FIBROBLAST; FLUORESCENCE; GENE EXPRESSION; GENETIC TRANSFECTION; HOST CELL; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; QUANTITATIVE ASSAY; ULTRAVIOLET RADIATION;

EID: 0034672320     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4865     Document Type: Article

References (34)

1. Variability in DNA repair and individual susceptibility to genotoxins
2. Somatic mutation theory, DNA repair rates, and molecular epidemiology of p53 mutations
3. The role of DNA repair capacity in susceptibility to lung cancer
4. Deficient DNA repair capacity: A predisposing factor and high risk predictive marker in familial colorectal cancer
5. Molecular epidemiology in cancer research
6. Assessment of DNA damage and repair in human peripheral blood mononuclear cells using novel DNA unwinding technique
7. 6-methylguanine-DNA methyltransferase levels with survival of malignant astrocytoma patients treated with bis-chloroethylnitrosourea: A Southwest Oncology Group study
8. DNA repair in an active gene: Removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall
9. Determination of steady-state levels of oxidative DNA base modifications in mammalian cells by means of repair endonucleases
10. Sensitive analysis of oxidative DNA damage in mammalian cells: Use of bacterial fpg protein in combination with alkaline unwinding
11. Oxidative DNA damage, antioxidants, and cancer
12. PCR assay of DNA damage and repair at the gene level in brain and spleen of gamma-irradiated young and old rats
13. Measurement of DNA damage and repair in the lambda LIZ transgene in a Big Blue rat cell line by quantitative PCR
14. Deficient nucleotide excision repair activity in protein extracts from normal human lymphocytes
15. DNA damage excision repair in microplate wells with chemiluminescence detection: Development and perspectives
16. DNA repair activity in protein extracts of fresh human malignant lymphoid cells
17. The comet assay: What can it really tells us?
18. Biomonitoring of occupational exposure to styrene in a plastic lamination plant
19. Biomonitoring of biotoxic risk in workers in rubber factory: Comparison of the Comet assay with cytogenetic methods and immunology
20. Host cell reactivation by human cells of DNA expression vectors damaged by ultraviolet radiation or by acid-heat treatment
21. Host cell reactivation of CAT-expression vectors as a method to assay for cloned DNA-repair genes
22. Development and field-test validation of an assay for DNA repair in circulating human lymphocytes
23. Cisplatin-DNA damage and repair in peripheral blood leukocytes in vivo and in vitro
24. Abnormal DNA repair activity in lymphocytes of workers exposed to 1,3-butadiene
25. Analysis if UV-B-induced DNA damage and its repair in heat-shocked skin cells
26. High efficiency transformation of Escherichia coli with plasmids
27. High-efficiency transformation of mammalian cells by plasmid DNA
28. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy
29. Expression and analysis of green fluorescent protein in human embryonic kidney cells by capillary electrophoresis
30. Transcription coupled repair removes both cyclobutane pyrimidine dimers and 6-4 photoproducts with equal efficiency and in sequential way from transcribed DNA in xeroderma pigmentosum group C fibroblasts
31. Induction and repair of DNA damage in UV irradiated human lymphocytes. Spectral differences and repair kinetics
32. Sequence effect on alkali-sensitive sites in UV-irradiated SV40 DNA
33. Reduced formation of bipyrimidine photoproducts in DNA UV irradiated at high intensity
34. Formation of the main UV-induced thymine dimeric lesions within isolated and cellular DNA as measured by high performance liquid chromatography-tandem mass spectrometry