Sensitive analysis of oxidative DNA damage in mammalian cells: Use of the bacterial Fpg protein in combination with alkaline unwinding

Toxicology Letters

Volumn 88, Issue 1-3, 1996, Pages 85-90

  Hartwig, Andrea ^a   Dally, Heike ^a   Schlepegrell, Regina ^a  

^a UNIVERSITY OF BREMEN   (Germany)

Author keywords

8 hydroxyguanine; alkaline unwinding; ferric nitrilotriacetate; Fpg protein; mammalian cells; oxidative DNA damage

Indexed keywords

8 HYDROXYGUANINE; DNA GLYCOSYLTRANSFERASE; PROTEIN;

ANIMAL CELL; CELL STRAIN V 79; CONFERENCE PAPER; CONTROLLED STUDY; DNA DAMAGE; DNA DENATURATION; DNA STRAND BREAKAGE; HELA CELL; HUMAN; HUMAN CELL; NONHUMAN; OXIDATION; PRIORITY JOURNAL;

ANIMALIA; BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0030297062     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-4274(96)03722-8     Document Type: Conference Paper

References (18)

1. [1] Halliwell, B. and Gutteridge, J.M.C. (1990) Role of free radicals and catalytic metal ions in human disease: an overview. In: L. Packer and A.E. Glazer (Eds.), Methods in Enzymology, Vol. 186. Academic Press, New York, pp. 1-85.
2. [2] Teebor, G.W., Boorstein, R.J. and Cadet, J. (1988) The repairability of oxidative free radical mediated damage to DNA: a review. Int. J. Radiat. Biol. 54, 131-150.
3. [3] Wood, M.L., Dizdaroglu, M., Gajewski, E. and Essigman, J.M. (1990) Mechanistic studies of ionizing radiation and oxidative mutagenesis: genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genome. Biochemistry 29, 7024-7032.
4. [4] Floyd, R.A. (1990) The role of 8-hydroxyguanine in carcinogenesis. Carcinogenesis 11, 1447-1450.
5. [5] Halliwell, B. and Dizdaroglu, M. (1992) The measurement of oxidative damage to DNA by HPLC and GC/MS techniques. Free Rad. Res. Commun. 16, 75-87.
6. [6] Epe, B. and Hegler, J. (1994) Oxidative DNA damage: endonuclease fingerprinting. In: L. Packer (Ed.), Methods in Enzymology, Vol. 234. Academic Press, New York, pp. 122-131.
7. [7] Boiteux, S., Gajewski, E., Laval, J. and Dizdaroglu, M. (1992) Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitization. Biochemistry 31, 106-110.
8. [8] Tchou, J., Kasai, H., Shibutani, S., Chung, M.-H., Laval, J., Grollman, A.P. and Nishimura, S. (1991) 8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity. Proc. Natl. Acad. Sci. USA 88, 4690-4694.
9. [9] Hartwig, A., Klyszcz-Nasko, H., Schlepegrell, R. and Beyersmann, D. (1993) Cellular damage by ferric nitrilotriacetate and ferric citrate in V79 cells: interrelationship between lipid peroxidation, DNA strand breaks and sister-chromatid exchanges. Carcinogenesis 14, 107-112.
10. [10] Kasten, U., Beyersmann, D., Dahm-Daphi, J. and Hartwig, A. (1995) Sensitive nonradioactive detection of UV-induced cyclobutane pyrimidine dimers in intact mammalian cells. Mutat. Res. 336, 143-152.
11. [11] Föhe, C. and Dikomey, E. (1994) Induction and repair of DNA base damage studied in X-irradiated CHO cells using the M. luteus extract. Int. J. Radiat. Biol. 66, 697-704.
12. [12] Ahnström, G. and Edvardsson, K.A. (1974) Radiation-induced single-strand breaks in DNA determined by rate of alkaline strand separation and hydroxyapatite chromatography: an alternative to velocity sedimentation. Int. J. Radiat. Biol. 26, 493-497.
13. [13] Goodhead, D.T. (1989) The initial physical damage produced by ionizing radiations. Int. J. Radiat. Biol. 56, 623-634.
14. [14] Roots, R., Holley, W., Chatterjee, A., Irizarry, M. and Kraft, G. (1990) The formation of strand breaks in DNA after high-LET irradiation: a comparison of data from in vitro and cellular systems. Int. J. Radiat. Biol. 58, 55-69.
15. [15] Floyd, R.A., West, M.S., Eneff, K.L. and Schneider, J.E. (1989) Methylene blue plus light mediates 8-hydroxyguanine formation in DNA. Arch. Biochem. Biophys. 273, 106-111.
16. [16] Schneider, J.E., Price, S., Maidt, M.L., Gutteridge, J.M.C. and Floyd, R.A. (1990) Methylene blue plus light mediates 8-hydroxy-2′-deoxyguanosine formation in DNA preferentially over strand breakage. Nucl. Acids Res. 18, 631-635.
17. [17] Epe, B., Pflaum, M. and Boiteux S. (1993) DNA damage induced by photosensitizers in cellular and cell-free systems. Mutat. Res. 299, 135-145.
18. [18] Hartwig, A. and Schlepegrell, R. (1995) Induction of oxidative DNA damage by ferric iron in mammalian cells. Carcinogenesis, 16, 3009-3013.