Toxicological investigations of the effects carboxymethylcellulose on ciliary beat frequency of human nasal epithelial cells in primary suspension culture and in vivo on rabbit nasal mucosa

International Journal of Pharmaceutics

Volumn 205, Issue 1-2, 2000, Pages 43-51

  Ugwoke, Michael Ikechukwu ^a   Agu, Remigius Uchenna ^a   Jorissen, Mark ^a   Augustijns, Patrick ^a   Sciot, Raf ^a   Verbeke, Norbert ^a   Kinget, Renaat ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Apomorphine; Carboxymethylcellulose; Ciliary beat frequency; Mucoadhesion; Mucosal inflammation; Nasal drug delivery

Indexed keywords

APOMORPHINE; CARBOXYMETHYLCELLULOSE; DRUG CARRIER;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CILIARY MOTILITY; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG FORMULATION; IN VIVO STUDY; INFLAMMATION; MICROSCOPY; NECROSIS; NONHUMAN; NOSE MUCOSA; PHOTOMETRY; PRIORITY JOURNAL; RABBIT; SQUAMOUS CELL METAPLASIA;

ANIMAL; CARBOXYMETHYLCELLULOSE; CELLS, CULTURED; CILIA; HUMAN; HYPERPLASIA; NASAL MUCOSA; RABBITS; SUPPORT, NON-U.S. GOV'T;

EID: 0034665269     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-5173(00)00484-1     Document Type: Article

References (17)

1. Agu R.U., Jorissen M., Willems T., Van Den Mooter G., Kinget R., Augustijns P. The effects of pharmaceutical compounds on ciliary beating in human nasal epithelial cells: a comparative study of cell culture models. Pharm. Res. 16:1999;1380-1385.
2. Aspden T.J., Mason J.D.T., Jones N.S., Lowe J., Skaugrud O., Illum L. Chitosan as a nasal delivery system: the effect of chitosan solutions on in vitro and in vivo mucociliary transport rates in human turbinates and volunteers. J. Pharm. Sci. 86:1997;509-513.
3. Behl C.R., Pimplaskar H.K., Sileno A.P., deMeireles J., Romeo V.D. Effects of physicochemical properties and other factors on systemic nasal drug delivery. Adv. Drug Del. Rev. 29:1998;89-116.
4. Björk E., Bjurstrom S., Edman P. Morphologic examination of rabbit nasal mucosa after nasal administration of degradable starch microspheres. Int. J. Pharm. 75:1991;73-80.
5. Chandler S.G., Illum L., Thomas W.N. Nasal absorption in rats. II. Effect of enhancers on insulin absorption and nasal histology. Int. J. Pharm. 76:1991;61-70.
6. Cornaz A.L., Buri P. Nasal mucosa as an absorption barrier. Eur. J. Pharm. Biopharm. 40:1994;261-270.
7. De Fraissinette A., Kollop M., Schiller I., Fricker G., Gammert C., Pospischil A., Vondersher J., Richter F. In vitro tolerability of human nasal mucosa: histopathological and scanning electron microscopic evaluation of nasal forms containing Sandostatin® Cell Biol. Toxicol. 11:1995;295-301.
8. Jiménez-Castellanos M.R., Zia H., Rhodes C.T. Mucoadhesive drug delivery systems. Drug Dev. Ind. Pharm. 19:1993;143-194.
9. Jorissen M., Van der Schueren B., Cassiman J.J. The preservation and regeneration of cilia on human nasal epithelial cells cultured in vitro. Arch. Otorhinolaryngol. 246:1989;308-314.
10. Jorissen M., De Brouwer J., Bessems A., Cassiman J.J. Quantification of ciliary beat frequency by computerized microscope photometry - a preliminary study on suspension cultures of human nasal epithelia showing spontaneous ciliogenesis in vitro. Sci. Tech. Inform. 10:1992;88-93.
11. Leußen H.L., Lehr C.M., Rentel C.O., Noach A.B.J., de Boer A.G., Verhoef J.C., Junginger H.E. Bioadhesive polymers for the peroral delivery of peptide drugs. J. Controlled Release. 29:1994;329-338.
12. Pereswetoff-Morath L., Bjurstrom S., Khan R., Dahlin M., Edman P. Toxicological aspects of the use of dextran microspheres and thermogelling ethyl(hydroxyethyl)cellulose (EHEC) an nasal drug delivery systems. Int. J. Pharm. 128:1996;9-21.
13. Richardson J.L., Thomas N.W., Illum L. Recovery of rat vaginal epithelium from the histopathological effects of absorption enhancers. Int. J. Pharm. 77:1991;75-78.
14. Romeijn S.G., Verhoef J.C., Marttin E., Merkus F.W.H.M. The effect of nasal drug formulations on ciliary beating in vitro. Int. J. Pharm. 135:1996;137-145.
15. Ugwoke, M.I., Agu, R.U., Jorissen, M., Augustijns, P., Sciot, R., Verbeke, N., Kinget, R., 1999a. Nasal toxicological investigations of Carbopol 971P formulation of apomorphine: effects on ciliary beat frequency of human nasal primary cell culture and in vivo on rabbit nasal mucosa. Eur. J. Pharm. Sci., in press.
16. Ugwoke, M.I., Kaufmann, G., Verbeke, N., Kinget, R., 1999b. Intranasal bioavailability of apomorphine from carboxymethylcellulose-based drug delivery systems. Int. J. Pharm., submitted for publication.
17. Zhou M., Donovan M.D. Recovery of the nasal mucosa following laureth-9 induced damage. Int. J. Pharm. 130:1996;93-102.