Six molecules of SV40 large T antigen assemble in a propeller-shaped particle around a channel

Journal of Molecular Biology

Volumn 268, Issue 1, 1997, Pages 15-20

  San Martín, M Carmen ^a   Gruss, Claudia ^b   Carazo, José M ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

DNA replication; Electron microscopy; Structure of helicases; Sv40 large T antigen; Three dimensional reconstruction

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; HELICASE; REGULATOR PROTEIN; SINGLE STRANDED DNA; VIRUS DNA; VIRUS LARGE T ANTIGEN; VIRUS PROTEIN;

ALGORITHM; ARTICLE; DNA DENATURATION; DNA REPLICATION; ELECTRON MICROSCOPY; IMAGE PROCESSING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SIMIAN VIRUS 40; STAINING; VIRUS INFECTION;

EID: 0031585985     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.0952     Document Type: Editorial

References (34)

1. Braithwaite A., Stürzbecher H., Addison C., Palmer C., Rudge K., Jenkins J. R. Mouse p53 inhibits SV40 origin-dependent DNA replication. Nature. 329:1987;458-460.
2. Bujalowski W., Jezewska M. J. Interactions of Escherichia coli primary replicative helicase DnaB protein with single-stranded DNA. The nucleic acid does not wrap around the protein hexamer. Biochemistry. 34:1995;8513-8519.
3. Bujalowski W., Klonowska M. M., Jezewska M. J. Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein. J. Biol. Chem. 269:1994;31350-31358.
4. Collins K. L., Kelly T. J. Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase α-primase. Mol. Cell. Biol. 11:1991;2108-2115.
5. Crowther R. A., Amos L. A. Harmonic analysis of electron microscope images with rotational symmetry. J. Mol. Biol. 60:1971;123-130.
6. Dean F. B., Hurwitz J. Simian virus 40 large T antigen untwists DNA at the origin of DNA replication. J. Biol. Chem. 266:1991;5062-5071.
7. Dean F. B., Borowiec J. A., Toshihiko E., Hurwitz J. The simian virus 40 T antigen double hexamer assembles around the DNA at the replication origin. J. Biol. Chem. 267:1992;14129-14137.
8. DeCaprio J. A., Ludlow J. W., Figge J., Shew J. Y., Huang C. M., Lee W. H., Marsillo E., Paucha E., Livingston D. M. SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene. Cell. 54:1988;275-283.
9. Dodson M., Dean F. B., Bullock P., Echols H., Hurwitz J. Unwinding of duplex DNA from the SV40 origin of replication by T antigen. Science. 238:1987;964-967.
10. Dong F., Gogol E. P., von Hippel P. H. The phage T4-coded DNA replication helicase (gp41) forms a hexamer upon activation by nucleoside triphosphate. J. Biol. Chem. 270:1995;7462-7473.
11. Egelman E. H., Yu X., Wild R., Hingorani M. M., Patel S. S. Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases. Proc. Natl Acad. Sci. USA. 92:1995;3869-3873.
12. Fairman M. P., Stillman B. Cellular factors required for multiple stages of SV40 DNA replication in vitro. EMBO J. 7:1988;1211-1218.
13. Fanning E., Knippers R. Structure and function of simian virus 40 large tumor antigen. Annu. Rev. Biochem. 61:1992;55-85.
14. Frank J., Radermacher M., Wagenknecht T., Verschoor A. Studying ribosome structure by electron microscopy and computer image processing. Methods Enzymol. 164:1988;3-35.
15. Llorca O., Marco S., Carrascosa J. L., Valpuesta J. M. Conformational changes in the GroEL oligomer during the functional cycle. J. Struct. Biol. 1997.
16. Marabini R., Carazo J. M. Pattern recognition and classification of images of biological macromolecules using artificial neural networks. Biophys. J. 66:1994;1804-1814.
17. Marabini R., Masegosa I. M., San Martin M. C., Marco S., Fernandez J. J., de la Fraga L. G., Vaquerizo C., Carazo J. M. Xmipp: an X-Windows based image processing package for electron microscopy. J. Struct. Biol. 116:1996;237-240.
18. Marabini R, Herman G. T., Carazo J. M. 3D reconstruction in electron microscopy using ART. 11th European Congress on Electron Microscopy, Dublin 1996. 1996;EUREM 96.
19. Marco S., Chagoyen M., Fraga L. G., Carazo J. M., Carrascosa J. L. A variant to the "random approximation" of the reference-free alignment algorithm. Ultramicroscopy. 1997.
20. Mastrangelo I. A., Hough P. V. C., Wall J. S., Dodson M., Dean F. B., Hurwitz J. ATP-dependent assembly of double hexamers of SV40 T antigen at the viral origin of DNA replication. Nature. 338:1989;658-662.
21. Mastrangelo I. A., Bezanilla M., Hansma P. K., Hough P. V. C., Hansma H. G. Structures of large T antigen at the origin of SV40 DNA replication by atomic force microscopy. Biophys. J. 66:1994;293-298.
22. Matson S. W., Bean D. W., George J. W. DNA helicases: enzymes with essential roles in all aspects of DNA metabolism. Bioessays. 16:1994;13-22.
23. Penczek P., Radermacher M., Frank J. Three-dimensional reconstruction of single particles embedded in ice. Ultramicroscopy. 40:1992;33-53.
24. Radermacher M., Wagenknecht T., Verschoor A., Frank J. Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli. J. Microsc. 146:1987;113-136.
25. San Martin M. C., Stamford N. P. J., Dammerova N., Dixon N. E., Carazo J. M. A structural model for the Escherichia coli DnaB helicase based on electron microscopy data. J. Struct. Biol. 114:1995;167-176.
26. Simanis V., Lane D. P. An immunoaffinity purification procedure for SV40 large T antigen. Virology. 144:1985;88-100.
27. Stahl H., Dröge P., Knippers R. DNA helicase activity of SV40 large tumor antigen. EMBO J. 5:1986;1939-1944.
28. Stasiak A., Tsaneva I. R., West S. C., Benson C. J. B., Yu X., Egelman E. H. The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA. Proc. Natl Acad. Sci. USA. 91:1994;7618-7622.
29. Tsurimoto T., Melendy T., Stillman B. Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin. Nature. 346:1990;534-539.
30. Unser M., Trus B., Steven A. C. A new resolution criterion based on spectral signal-to-noise ratios. Ultramicroscopy. 23:1987;39-52.
31. Valle M., Valpuesta J. M., Carrascosa J. L., Tamayo J., Garcia R. The interaction of DNA with bacteriophage φ29 connector: a study by AFM and TEM. J. Struct. Biol. 116:1996;390-398.
32. Valpuesta J. M., Carrascosa J. L. Structure of viral connectors and their function in bacteriophage assembly and DNA packaging. Quart. Rev. Biophys. 27:1994;107-155.
33. Wang E. H., Friedman P., Prives N. The murine p53 protein blocks replication of SV40 DNA in vitro by inhibiting the initiation functions of the SV40 large T antigen. Cell. 37:1989;801-813.
34. Wessel R., Schweizer J., Stahl H. Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication. J. Virol. 66:1992;804-815.