Regulation of area identity in the mammalian neocortex by Emx2 and Pax6

Science

Volumn 288, Issue 5464, 2000, Pages 344-349

  Bishop, Kathie M ^a   Goudreau, Guy ^b   O'Leary, Dennis D M ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HOMEODOMAIN PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN CELL; BRAIN REGION; BRAIN VENTRICLE; CONTROLLED STUDY; EMBRYO; GENE EXPRESSION REGULATION; MOUSE MUTANT; NEOCORTEX; NONHUMAN; PRIORITY JOURNAL; REGULATOR GENE; THALAMUS;

ANIMALS; BRAIN MAPPING; CADHERINS; DNA-BINDING PROTEINS; EYE PROTEINS; GENE EXPRESSION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, HOMEOBOX; GENES, REGULATOR; HOMEODOMAIN PROTEINS; MICE; MICE, INBRED C57BL; MICE, INBRED DBA; MICE, MUTANT STRAINS; MORPHOGENESIS; NEOCORTEX; NEURAL PATHWAYS; OCCIPITAL LOBE; PAIRED BOX TRANSCRIPTION FACTORS; REPRESSOR PROTEINS; SOMATOSENSORY CORTEX; THALAMUS; TRANSCRIPTION FACTORS; VISUAL CORTEX;

ANIMALIA; MAMMALIA;

EID: 0034646993     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5464.344     Document Type: Article

References (49)

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22. Embryonic day 18.5 (E18.5) embryos were obtained from timed-pregnant matings of Emx2 heterozygous mice maintained on a C57BL/6J background (E0.5 = noon of day of vaginal plug detection). Animal care was in accordance with institutional guidelines. Littermate sets of cortices from wild type, heterozygote, and homozygous mutants were analyzed to control for differences in developmental rate. Whole-mount in situ hybridization of cortices {modified from work by D. Henrique et al., [Nature 375, 787 (1995)]} used digoxygeninlabeled riboprobes encoding Cad6 and Cad8. Emx2 mice were genotyped by polymerase chain reaction on tail DNA. Whole-mount in situ hybridizations were quantified with the Image program from the National Institutes of Health. Quantification was done blinded to genotype.
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34. E18.5 Emx2 embryos were collected as in (13). AChE histochemistry was as in work by B. L. Schlaggar, J. A. De Carlos and D. D. M. O'Leary [Dev. Brain Res. 75, 19 (1993)]. In situ hybridization and counterstaining were as in work by R. Tuttle, Y. Nakagawa, J. E. Johnson and D. D. M. O'Leary [Development 126, 1903 (1999)].
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49. We are grateful to P. Gruss for the gift of Emx2 mice, M. Goulding for Sey mice and Pax6 plasmid, and Y. Nakagawa for Cad6, Cad8, and Gbx2 plasmids and helpful discussions. This work was supported by NIH grant NS31558 (D.D.M.O'L), the Natural Sciences and Engineering Research Council of Canada (K.M.B.), and the Max-Planck Society and European Commission grant BI04-CT96-0378 (G.G.).