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Immunohistochemistry on 10-μm cryostat sections was performed as described in (76) with rabbit antibodies to 5HT (1:5000), Incstar P450c17 (1:1000) (16), or calretinin (1:2000) (Chemicon) revealed with biotinylated goat antibody to rabbit 1:200 (Vector), followed by peroxidase staining with the VIP kit (Vector)
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Immunohistochemistry on 10-μm cryostat sections was performed as described in (76) with rabbit antibodies to 5HT (1:5000), Incstar P450c17 (1:1000) (16), or calretinin (1:2000) (Chemicon) revealed with biotinylated goat antibody to rabbit 1:200 (Vector), followed by peroxidase staining with the VIP kit (Vector).
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40
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0345003020
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Each probe was tested on two to three different mutants and four different wild-type animals; typically, eight to nine different section planes were studied from each brain with each probe
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Each probe was tested on two to three different mutants and four different wild-type animals; typically, eight to nine different section planes were studied from each brain with each probe.
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41
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0345434130
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We thank G. Martin for the Gbx-2 mutant mice, S. Mellon for antiserum to P450c17, M. Takeichi for cadherin-6, R. Grosschedl for Lef-1, M. Israel for Id-4, S. McConnell for RZR-beta, and A. Wakana for EphA-7 plasmids. This work was supported by the research grants to J.LR.R. from: Nina Ireland, March of Dimes, the National Alliance for Research on Schizophrenia and Depression, the John Merck Fund, the Human Frontiers Science Program, the National Institute of Neurological Disorders and Stroke (NINDS) (grant NS34661-01A1) and the National Institute of Mental Health (grants RO1 MH49428-01, RO1 MH51561-01A1, and K02 MH01046-01), to R.F.H. from NINDS (grant K08NS01973), and to E.M.M. from NINDS (grant 1 F32 NS10536-01)
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We thank G. Martin for the Gbx-2 mutant mice, S. Mellon for antiserum to P450c17, M. Takeichi for cadherin-6, R. Grosschedl for Lef-1, M. Israel for Id-4, S. McConnell for RZR-beta, and A. Wakana for EphA-7 plasmids. This work was supported by the research grants to J.LR.R. from: Nina Ireland, March of Dimes, the National Alliance for Research on Schizophrenia and Depression, the John Merck Fund, the Human Frontiers Science Program, the National Institute of Neurological Disorders and Stroke (NINDS) (grant NS34661-01A1) and the National Institute of Mental Health (grants RO1 MH49428-01, RO1 MH51561-01A1, and K02 MH01046-01), to R.F.H. from NINDS (grant K08NS01973), and to E.M.M. from NINDS (grant 1 F32 NS10536-01).
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