Use of inhibitory monoclonal antibodies to assess the contribution of cytochromes P450 to human drug metabolism

European Journal of Pharmacology

Volumn 394, Issue 2-3, 2000, Pages 199-209

  Shou, Magang ^a   Lu, Tin ^b   Krausz, Kristopher W ^b   Sai, Yang ^b   Yang, Tianjian ^b   Korzekwa, Kenneth R ^c   Gonzalez, Frank J ^b   Gelboin, Harry V ^b  

^a MERCK RESEARCH LABORATORIES   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c Camitro Corporation   (United States)

Author keywords

cDNA expression; Cytochrome P450; Drug metabolism; human; Inhibition; Liver microsome; Monoclonal antibody

Indexed keywords

4 NITROANISOLE DEMETHYLASE; AFLATOXIN B1; CHLORZOXAZONE; COMPLEMENTARY DNA; CYTOCHROME P450 3A; CYTOCHROME P450 3A4; CYTOCHROME P450 ISOENZYME; DIAZEPAM; DIMETHYLNITROSAMINE DEMETHYLASE; ENZYME ANTIBODY; MONOCLONAL ANTIBODY; N DEMETHYLASE; PACLITAXEL; RECOMBINANT ENZYME; TESTOSTERONE; TOLUENE;

ADULT; ARTICLE; CONTROLLED STUDY; DEMETHYLATION; DRUG METABOLISM; ENZYME INHIBITION; ENZYME SUBSTRATE; FEMALE; GAS CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; HYDROXYLATION; LIVER MICROSOME METABOLISM; MALE; MASS SPECTROMETRY; PHENOTYPE; PRIORITY JOURNAL; TESTOSTERONE METABOLISM;

ANTIBODIES, BLOCKING; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME INHIBITORS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENETIC VECTORS; HUMANS; ISOENZYMES; MICROSOMES, LIVER; PHARMACEUTICAL PREPARATIONS; VACCINIA VIRUS;

EID: 0034646948     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(00)00079-0     Document Type: Article

References (35)

1. Chang T.K.H., Gonzalez F.J., Waxman D.J. Evaluation of triacetyloleandomycin, α-naphthoflavone and diethyldithiocarbamate as selective chemical probes for inhibition of human cytochromes P450. Arch. Biochem. Biophys. 311:1994;422-437.
2. Friedman F.K., West D., Dewick P.M., Gelboin H.V. Specificity of medicarpin and related flavones in inhibition of rat hepatic mixed function oxidase activity. Pharmacology. 31:1985;289-293.
3. Gelboin H.V. Cytochrome P450 and monoclonal antibodies. Pharmacol. Rev. 45:1993;413-453.
4. Gelboin H.V., Goldfarb I., Krausz K.W., Grogan J., Korzekwa K.R., Gonzalez F.J., Shou M. Inhibitory and noninhibitory monoclonal antibodies to human cytochrome P450 2E1. Chem. Res. Toxicol. 9:1996;1023-1030.
5. Gelboin H.V., Krausz K.W., Goldfarb I., Buters J.T., Yang S.K., Gonzalez F.J., Korzekwa K.R., Shou M. Inhibitory and non-inhibitory monoclonal antibodies to human cytochrome P450 3A3/4. Biochem. Pharmacol. 50:1995;1841-1850.
6. Gelboin H.V., Krausz K.W., Shou M., Gonzalez F.J., Yang T.J. A monoclonal antibody inhibitory to human P450 2D6: a paradigm for use in combinatorial determination of individual P450 role in specific drug tissue metabolism. Pharmacogenetics. 7:1997;469-477.
7. Gonzalez F.J. The molecular biology of cytochrome P450s. Pharmacol. Rev. 40:1988;243-288. [published erratum appears in Pharmacol Rev 1989 Mar; l41(1):91-92].
8. Gonzalez F.J., Aoyama T., Gelboin H.V. Activation of promutagens by human cDNA-expressed cytochrome P450s. Prog. Clin. Biol. Res. 340b:1990;77-86.
9. Gonzalez F.J., Aoyama T., Gelboin H.V. Expression of mammalian cytochrome P450 using vaccinia virus. Methods Enzymol. 206:1991b;85-92.
10. Gonzalez F.J., Crespi C.L., Gelboin H.V. DNA-expressed human cytochrome P450s: a new age of molecular toxicology and human risk assessment. Mutat. Res. 247:1991c;113-127.
11. Gonzalez F.J., Kimura S., Gelboin H.V. Expression of mammalian cytochrome P450 using baculovirus. Methods Enzymol. 206:1991b;93-99.
12. Gonzalez F.J., Korzekwa K.R. Cytochromes P450 expression systems. Annu. Rev. Pharmacol. Toxicol. 35:1995;369-390.
13. Gorski J.C., Jones D.R., Wrighton S.A., Hall S.D. Contribution of human P4503A subfamily members to the 6-hydroxylation of chlorzoxazone. Xenobiotica. 27:1997;243-256.
14. Guengerich F.P. Catalytic selectivity of human cytochrome P450 enzymes: relevance to drug metabolism and toxicity. Toxicol. Lett. 70:1994;133-138.
15. Guengerich F.P., Shimada T., Bondon A., Macdonald T.L. Cytochrome P-450 oxidations and the generation of biologically reactive intermediates. Adv. Exp. Med. Biol. 283:1991;1-11.
16. Guengerich F.P., Shimada T., Iwasaki M., Butler M.A., Kadlubar F.F. Activation of carcinogens by human liver cytochromes P-450. Basic Life Sci. 53:1990;381-396.
17. Hanioka N., Korzekwa K., Gonzalez F.J. Sequence requirements for cytochromes P450IIA1 and P450IIA2 catalytic activity: evidence for both specific and non-specific substrate binding interactions through use of chimeric cDNAs and cDNA expression. Protein Eng. 3:1990;571-575.
18. Harris J.W., Rahman A., Kim B.R., Guengerich F.P., Collins J.M. Metabolism of taxol by human hepatic microsomes and liver slices: participation of cytochrome P450 3A4 and an unknown P450 enzyme. Cancer Res. 54:1994;4026-4035.
19. Hooper W.D., Watt J.A., McKinnon G.E., Reilly P.E. Metabolism of diazepam and related benzodiazepines by human liver microsomes. Eur. J. Drug Metab. Pharmacokinet. 17:1992;51-59.
20. Jung F., Richardson T.H., Raucy J.L., Johnson E.F. Diazepam metabolism by cDNA-expressed human 2C P450s: identification of P4502C18 and P4502C19 as low K(M) diazepam N-demethylases. Drug Metab. Dispos. 25:1997;133-139.
21. Ono S., Hatanaka T., Hotta H., Tsutsui M., Satoh T., Gonzalez F.J. Chlorzoxazone is metabolized by human P4501A2 as well as by human P4502E1. Pharmacogenetics. 5:1995;143-150.
22. Ono S., Hatanaka T., Miyazawa S., Tsutsui M., Aoyama T., Gonzalez F.J., Satoh T. Human liver microsomal diazepam metabolism using cDNA-expressed cytochrome P450s: role of P4502B6, 2C19 and the 3A subfamily. Xenobiotica. 26:1996;1155-1166.
23. Park S.S., Waxman D.J., Lapenson D.P., Schenkman J.B., Gelboin H.V. Monoclonal antibodies to rat liver cytochrome P450-2c/RLM5 that regiospecifically inhibit steroid metabolism. Biochem. Pharmacol. 38:1989;3067-3074.
24. Patten C.J., Thomas P.E., Buy R.L., Lee M., Gonzalez F.J., Guengerich F.P., Yang C.S. Cytochrome P450 enzymes involved in acetaminophen activation by rat and human liver microsomes and their kinetics. Chem. Res. Toxicol. 6:1993;511-518.
25. Peter R., Bocker R., Beaune P.H., Iwasaki M., Guengerich F.P., Yang C.S. Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1. Chem. Res. Toxicol. 3:1990;556-573.
26. Raney K.D., Shimada T., Kim D.H., Groopman J.D., Harris T.M., Guengerich F.P. Oxidation of aflatoxins and sterigmatocystin by human liver microsomes: significance of aflatoxin Q1 as a detoxication product of aflatoxin B1. Chem. Res. Toxicol. 5:1992;202-210.
27. Reilly P.E., Thompson D.A., Mason S.R., Hooper W.D. Cytochrome P450IIIA enzymes in rat liver microsomes: involvement in C3-hydroxylation of diazepam and nordazepam but not N-dealkylation of diazepam and temazepam. Mol. Pharmacol. 37:1990;767-774.
28. Sai Y., Dai R., Yang T.J., Rushmore R.H., Baillie T.A., Gonzalez F.J., Gelboin H.V., Shou M. Inhibition kinetics of monoclonal antibodies against cytochrome P450-mediated drug metabolism. FASEB J. 12:1998;A146.
29. Tassaneeyakul W., Birkett D.J., Veronese M.E., McManus M.E., Tukey R.H., Quattrochi L.C., Gelboin H.V., Miners J.O. Specificity of substrate and inhibitor probes for human cytochromes P4501A1 and 1A2. J. Pharmacol. Exp. Ther. 265:1993;401-407.
30. Ueng Y.F., Shimada T., Yamazaki H., Guengerich F.P. Oxidation of aflatoxin B1 by bacterial recombinant human cytochrome P450 enzymes. Chem. Res. Toxicol. 8:1995;218-225.
31. Wang P.P., Beaune P., Kaminsky L.S., Dannan G.A., Kadlubar F.F., Larrey D., Guengerich F.P. Purification and characterization of six cytochrome P-450 isozymes from human liver microsomes. Biochemistry. 22:1983;5375-5383.
32. Yamazaki H., Guo Z., Guengerich F.P. Selectivity of cytochrome P4502E1 in chlorzoxazone 6-hydroxylation. Drug Metab. Dispos. 23:1995;438-440.
33. Yamazaki H., Inui Y., Yun C.H., Guengerich F.P., Shimada T. Cytochrome P450 2E1 and 2A6 enzymes as major catalysts for metabolic activation of N-nitrosodialkylamines and tobacco-related nitrosamines in human liver microsomes. Carcinogenesis. 13:1992;1789-1794.
34. Yang T.J., Sai Y., Krausz K.W., Gonzalez F.J., Gelboin H.V. Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver. Pharmacogenetics. 8:1998;375-382.
35. Yang T.J., Shou M., Korzekwa K.R., Gonzalez F.J., Gelboin H.V. Role of cDNA-expressed human cytochromes P450 in the metabolism of diazepam. Biochem. Pharmacol. 55:1998;889-896.