Probing the catalytic mechanism of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase by kinetic and crystallographic characterization of site-specific mutants

Journal of Molecular Biology

Volumn 303, Issue 1, 2000, Pages 77-91

  Rosano, Camillo ^a   Bisso, Angela ^b   Izzo, Gaetano ^a   Tonetti, Michela ^b   Sturla, Laura ^b   De Flora, Antonio ^b   Bolognesi, Martino ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF GENOA   (Italy)

Author keywords

Enzyme structure; Epimerization; GDP L fucose; NADP+; Short chain dehydrogenase

Indexed keywords

CYSTEINE; EPIMERASE; GUANOSINE DIPHOSPHATE; HISTIDINE; HOLOENZYME; LYSINE; MANNOSE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; SERINE; TYROSINE;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ISOMERISM; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTON TRANSPORT; REDUCTION; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS);

EID: 0034644773     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4106     Document Type: Article

References (55)

1. Molecular mimicry between Helicobacter pylori and the host
2. The refined crystal structure of Drosophilia lebanonensis alcohol dehydrogenase at 1.9 Å resolution
3. The Protein Data Bank
4. Structural and enzymatic characterization of human recombinant GDP-D-mannose-4,6-dehydratase
5. Alpha/Beta structures
6. Carbohydrate ligands of the LEC cell adhesion molecules
7. + identifies two principal targets for the design of inhibitors
8. 15N NMR and kinetic study
9. An epimerase-reductase in L-fucose synthesis
10. Monosaccharides
11. Site-specific mutagenesis of Drosophila alcohol dehydrogenase: Evidence for involvement of tyrosine-152 and lysine-156 in catalysis
12. Effect of site-directed mutagenesis on conserved positions of Drosophila alcohol dehydrogenase
13. Site directed mutagenesis of virtually any plasmid by eliminating a unique site
14. Accurate bond and angle parameters for X-ray structure refinement
15. +-dependent 15-hydroxyprostaglandin dehydrogenase to an alanine results in an inactive enzyme
16. Brief report: Recurrent severe infections caused by a novel leukocyte adhesion deficiency
17. The major blood group ABO(H) determining genes are isolated
18. Carbohydrates as antigenic determinants of glycoproteins
19. Formation of guanosine diphosphate L-fucose from guanosine diphosphate D-mannose
20. Studies on the biosynthesis of guanosine diphosphate L-fucose
21. The refined three-dimensional structure of 3α,20β-hydroxysteroid dehydrogenase and possible roles of the residues conserved in short-chain dehydrogenases
22. Structure of estrogenic 17b-hydrosysteroid dehydrogenase at 2.20 resolution
23. Improved methods for building protein models in electron density maps and the location of errors in these models
24. Short-chain dehydrogenase/reductase (SDR)
25. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
26. Structural comparisons lead to the definition of a new superfamily of NAD(P)(H)-accepting oxidoreductase: The single-domain reductase/epimerase/dehydrogenase (the 'RED' family)
27. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
28. Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coli
29. Selectin ligands, leukocyte trafficking, and fucosyltransferase genes
30. Stereochemical course and steady state mechanism of the reaction catalyzed by the GDP-fucose synthetase from Escherichia coli
31. Isolation and partial characterization of a NADP and NADPH-binding protein from human erythrocytes
32. Refinement of macromolecular structures by the maximum-likelihood method
33. Processing of X-ray diffraction data collected in oscillation mode
34. Characteristic of short-chain alcohol dehydrogenase and related enzymes
35. Neutrophil adhesion in leukocyte adhesion deficiency syndrome type 2
36. GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from Escherichia coli, a key enzyme in the biosynthesis of GDP-L-fucose, displays the structural characteristics of the RED protein homology super-family
37. The high resolution structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase
38. GDP-fucose synthetase from Escherichia coli: Structure of a unique member of the short-chain dehydrogenase/reductase family that catalyzes two distinct reactions at the same active site
39. Structural and kinetic analysis of Escherichia coli GDP-mannose 4,6-dehydratase provides insights into the enzyme's catalytic mechanism and regulation by GDP-fucose
40. Expression, purification and characterization of GDP-D-mannose 4,6-dehydratase from Escherichia coli
41. Molecular cloning of human GDP-mannose 4,6-dehydratase and reconstitution of GDP-fucose biosynthesis in vitro
42. Structure of the gene of turn-transplantation antigen P35B: Presence of a point mutation in the antigenic allele
43. Crystal structures of the binary and ternary complexes of 7α-hydroxy-steroid dehydrogenase from Escherichia coli
44. Crystal structure of the ternary complex of mouse lung carbonyl reductase at 1.8 Å resolution: The structural origin of coenzyme specificity in the short-chain dehydrogenase/reductase family
45. Crystal structure of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli
46. Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: Implications for the catalytic mechanism
47. High resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol
48. Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli
49. Synthesis of GDP-L-fucose by the human FX protein
50. The metabolism of 6-deoxyhexoses in bacterial and animal cells
51. Preliminary crystallographic investigations of recombinant GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from E. coli
52. Selectin ligands
53. 3-Lys-containing family of redictases and dehydrogenases
54. LIGPLOT: A program to generate schematic diagrams of protein-ligand interaction
55. Molecular and chemical characterization of the lipopolysaccharide O-antigen and its role in the virulence of Yersinia enterocolitica serotype O:8