Agrin-induced acetylcholine receptor clustering is mediated by the small guanosine triphosphatases Rac and Cdc42

Journal of Cell Biology

Volumn 150, Issue 1, 2000, Pages 205-212

  Weston, Christi ^a   Yee, Barry ^a   Hod, Eldad ^a   Prives, Joav ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Cdc42; Guanosine triphosphate binding proteins; Neuromuscular junction; Nicotinic acetylcholine receptors; Rac

Indexed keywords

AGRIN; CHOLINERGIC RECEPTOR; GUANOSINE TRIPHOSPHATASE; PROTEIN CDC42; RAC PROTEIN;

ANIMAL CELL; ARTICLE; MOTONEURON; MOUSE; MUSCLE FIBRIL; NEUROMUSCULAR SYNAPSE; NONHUMAN; PRIORITY JOURNAL; RECEPTOR AFFINITY; RECEPTOR BINDING; SIGNAL TRANSDUCTION; SYNAPTOGENESIS;

AGRIN; ANIMALS; CDC42 GTP-BINDING PROTEIN; CELL LINE; COS CELLS; GENES, DOMINANT; GTP PHOSPHOHYDROLASES; MICE; MUSCLE, SKELETAL; MUTAGENESIS, SITE-DIRECTED; MYOFIBRILS; RAC GTP-BINDING PROTEINS; RECEPTOR AGGREGATION; RECEPTORS, CHOLINERGIC; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 0034631836     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.1.205     Document Type: Article

References (35)

1. Apel, E.D., S.L. Roberds, K.P. Campbell, and J.P. Merlie. 1995. Rapsyn may function as a link between the acetylcholine receptor and the agrin-binding dystrophin-associated glycoprotein complex. Neuron. 15:115-126.
2. Apel, E., D. Glass, L. Moscoso, G. Yancopoulos, and J. Sanes. 1997. Rapsyn is required for MuSK signaling and recruits synaptic components to a MuSK containing scaffold. Neuron. 18:623-635.
3. Arber, S., F.A. Barbayannis, H. Hanser, C. Schneider, C.A. Stanyon, O. Bernard, and P. Caroni. 1998. Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase. Nature. 393:805-809.
4. Bowe, M.A., and J.R. Fallon. 1995. The role of agrin in synapse formation. Annu. Rev. Neurosci. 18:443-462.
5. Bowen, D.C., J. Sugiyama, M. Ferns, and Z.W. Hall. 1996. Neural agrin activates a high-affinity receptor in C2 muscle cells that is unresponsive to muscle agrin. J. Neurosci. 16:3791-3797.
6. Burden, S.J. 1998. The formation of neuromuscular synapses. Genes Dev. 12: 133-148.
7. Burridge, K., and M. Chrzanowska-Wodnicka. 1996. Focal adhesions, contractility, and signaling. Annu. Rev. Cell Dev. Biol. 12:463-518.
8. Coso, O.A., M. Chiariello, J.-C. Yu, H. Teramoto, P. Crespo, N. Xu, T. Miki, and J.S. Gutkind. 1995. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway. Cell. 81:1137-1146.
9. Dai, Z., and H.B. Peng. 1998. A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation. J. Cell Biol. 141:1613-1624.
10. DeChiara, T.M., D.C. Bowen, D.M. Valenzuela, M.V. Simmons, W.T. Poueymirou, S. Thomas, E. Kinetz, D.L. Compton, J.S. Park, C. Smith, et al. 1996. The receptor tyrosine kinase, MuSK, is required for neuromuscular junction formation in vivo. Cell. 85:501-512.
11. Ferns, M.J., J.T. Campanelli, W. Hoch, R.H. Scheller, and Z. Hall. 1993. The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron. 11:491-502.
12. Ferns, M., M. Deiner, and Z. Hall. 1996. Agrin-induced acetylcholine receptor clustering in mammalian muscle requires tyrosine phosphorylation. J. Cell Biol. 132:937-944.
13. Fuhrer, C., M. Gautam, J.E. Sugiyama, and Z.W. Hall. 1999. Roles of rapsyn and agrin in interaction of postsynaptic proteins with acetylcholine receptors. J. Neurosci. 19:6405-6416.
14. Gonzalez, M., F.P. Ruggiero, Q. Chang, Y.-J. Shi, M.M, Rich, S. Kraner, and R.J. Balice-Gordon. 1999. Disruption of TrkB-mediated signaling induces disassembly of postsynaplic receptor clusters at neuromuscular junctions. Neuron. 24:567-583.
15. Hall, A. 1998. Rho GTPases and the actin cytoskeleton. Science. 279:509-514.
16. Hazzalin, C.A., E. Cano, A. Cuenda, M.J. Barratt, P. Cohen, and L.C. Mahadevan. 1996. p38/RK is essential for stress-induced nuclear responses: JNK/SAPKs and c-Jun/ATF-2 phosphorylation are insufficient. Curr. Biol. 6:1028-1031.
17. Hoch, W., J.T. Campanelli, and R.H. Scheller. 1994. Agrin-induced clustering of acetylcholine receptors: a cytoskeletal link. J. Cell Biol. 126:1-4.
18. Joneson, T., M. McDonough, D. Bar-Sagi, and L. Van Aelst. 1996. Rac regulation of actin polymerization and proliferation by a pathway distinct from Jun kinase. Science. 274:1374-1376.
19. Keely, P., J.K. Westwick, I.P. Whitehead, C.J. Der, and L.V. Parise. 1997. Cdc42 and Rac1 induce integrin motility and invasiveness through PI(3)K. Nature. 390:632-636.
20. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685.
21. Manser, E., T. Leung, H. Salihuddin, Z.-S. Zhao, and L. Lim. 1994. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature. 367: 40-46.
22. Minden, A., A. Lin, F.-X. Claret, A. Abo, and M. Karin. 1995. Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs. Cell. 81:1147-1157.
23. Nimnual, A.S., B.A. Yatsula, and D. Bar-Sagi. 1998. Coupling of Ras and Rac guanosine triphosphatases through the Ras exchanger Sos. Science. 279: 560-563.
24. Prives, J., A.B. Fulton, S. Penman, M.P. Daniels, and C.N. Christian. 1982. Interaction of the cytoskeletal framework with acetylcholine receptor on the surface of embryonic muscle cells in culture. J. Cell Biol. 92:231-236.
25. Sander, E.E., S. van Delft, J.P. ten Klooster, T. Reid, R.A. van der Kammen, F. Michiels, and J.G. Collard. 1998. Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase. J. Cell Biol. 143:1385-1398.
26. Sanes, J.R., and J.W. Lichtman. 1999. Development of the vertebrate neuromuscular junction. Annu. Rev. Neurosci. 22:389-442.
27. Sells, M.A., J.T. Boyd, and J. Chernoff. 1999. p21-Activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts. J. Cell Biol. 145:837-849.
28. Shadiack, A.M., and R.M. Nitkin. 1991. Agrin induces α-actinin, filamin, and vinculin to co-localize with AChR clusters on cultured myotubes. J. Neurobiol. 22:617-628.
29. Spector, I., N.R. Shochet, D. Blasberger, and Y. Kashman. 1989. Cell Motil. Cytoskelet. 13:127-144.
30. Stya, M., and D. Axelrod. 1983. Mobility and detergent extractability of acetylcholine receptors on cultured rat myotubes: a correlation. J. Cell Biol. 97:48-51.
31. Tan, Y., J. Rouse, A. Zhang, S. Cariati, P. Cohen, and M.J. Comb. 1996. FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2. EMBO (Eur. Mol. Biol. Organ.) J. 15:4629-4642.
32. Tapon, N., and A. Hall. 1997. Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton. Curr. Opin. Cell Biol. 9:86-92.
33. Valenzuela, D.M., T.N. Stitt, P.S. DiStefano, E. Rojas, K. Mattsson, D.L. Compton, L. Lunez, J.S. Park, J.L. Stark, D.R. Gies, et al. 1996. Receptor tyrosine kinase specific for the skeletal muscle lineage: expression in embryonic muscle, at the neuromuscular junction, and after injury. Neuron. 15: 573-584.
34. Van Leeuwen, F.N., S. Van Delft, H.E. Kain, R.A. Van Der Kammen, and J.G. Collard. 1999. Rac regulates phosphorylation of the myosin-II heavy chain, actinomyosin disassembly and cell spreading. Nature Cell Biol. 1:242-248.
35. Yang, N., O. Higuchi, K. Ohashi, K. Nagata, A. Wada, K. Kangawa, E. Nishida, and K. Mizuno. 1998. Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature. 393:809-812.