The Vpu protein of human immunodeficiency virus type 1 forms cation- selective ion channels

Journal of Virology

Volumn 70, Issue 10, 1996, Pages 7108-7115

  Ewart, G D ^a   Sutherland, T ^a   Gage, P W ^a   Cox, G B ^a  

^a Biology and Environment   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CATION CHANNEL; VPU PROTEIN;

ARTICLE; CELL MEMBRANE TRANSPORT; CELLULAR DISTRIBUTION; CHANNEL GATING; CHLORIDE TRANSPORT; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; INTRACELLULAR TRANSPORT; NONHUMAN; PHOSPHATE TRANSPORT; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; SODIUM TRANSPORT; TRANSPORT KINETICS;

EID: 0029794387     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.10.7108-7115.1996     Document Type: Article

References (36)

1. Bour, S., U. Schubert, and K. Strebel. 1995. The human immunodeficiency virus type 1 Vpu protein specifically binds to the cytoplasmic domain of CD4: implications for the mechanism of degradation. J. Virol. 69:1510-1520.
2. Duff, K. C., and R. H. Ashley. 1992. The transmemhrane domain of influenza A M2 protein forms amantadine-sensitive proton channels in planar lipid bilayers. Virology 190:485-489.
3. Friborg, J., A. Ladha, H. Gottlinger, W. A. Haseltine, and E. A. Cohen. 1995. Functional analysis of the phosphorylation sites on the human immunodeficiency virus type 1 Vpu protein. J. Acquired Immune Defic. Syndr. Hum. Retrovirol. 8:10-22.
4. Haddock, B. A., and J. A. Downie. 1974. The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutants of Escherichia coli as demonstrated by quenching of atebrin fluorescence. Biochem. J. 142:703-706.
5. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
6. Hay, A. J., A. J. Wolstenholme, J. J. Skehel, and M. H. Smith. 1985. The molecular basis of the specific anti-influenza action of amantadine. EMBO J. 4:3021-3024.
7. Helenius, A. 1992. Unpacking the incoming influenza virus. Cell 69:577-578.
8. Jabbar, M. A. 1995. The human immunodeficiency virus type 1 Vpu protein: roles in virus release and CD4 downregulation. Curr. Top. Microhiol. Immunol. 193:107-120.
9. Klimkait, T., K. Strebel, M. D. Hoggan, M. A. Martin, and J. M. Orenstein. 1990 The human immunodeficiency virus type 1-specific protein Vpu is required for efficient virus maturation and release. J. Virol. 64:621-629.
10. Levy, J. A. 1993. Pathogenesis of human immunodeficiency virus infection. Microhiol. Rev. 57:183-289.
11. Love, C. A., P. E. Lilley, and N. E. Dixon. Stable high-copy-number bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene, in press.
12. Lu, Y.-A., P. Clavijo, M. Galantine, Z.-Y. Shen, W. Liu, and J. P. Tam. 1991. Chemically unambiguous peptide immunogen: preparation, orientation and antigenicity of purified peptide conjugated to the multiple antigen peptide system. Mol. Immunol. 28:623-630.
13. Maldarelli, F., M. V. Chen, R. L. Willey, and K. Strebel. 1993. Human immunodeficiency virus type-1 Vpu protein is an oligomeric type 1 integral membrane protein. J. Virol. 67:5056-5061.
14. Miller, C. 1986. Ion channel reconstitution. Plenum Press, New York.
15. New, R. C. C. (ed.). 1990. Liposomes: a practical approach. IRL Press, Oxford.
16. Piller, S. C., G. D. Ewart, A. Premkumar, G. B. Cox, and P. W. Gage. 1996. Vpr protein of human immunodeficiency virus type 1 forms cation-selective channels in planar lipid bilayers. Proc. Natl. Acad. Sci. USA 93:111-115.
17. Pinto, L. H., L. J. Holsinger, and R. A. Lamb. 1992. Influenza virus M2 protein has ion channel activity. Cell 69:517-528.
18. Rosen, B. R. 1987. ATP-coupled solute transport systems, p. 760-767. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
19. Sansom, M. S., and I. D. Kerr. 1993. Influenza virus M2 protein: a molecular modelling study of the ion channel. Protein Eng. 6:65-74.
20. Schroeder, C., C. M. Ford, S. A. Wharton, and A. J. Hay. 1994. Functional reconstitution in lipid vesicles of influenza virus M2 protein expressed by baculovirus: evidence for proton transfer activity. J. Gen. Virol. 75:3477-3484.
21. Schubert, U., S. Bour, A. V. Ferrermontiel, M. Montai, F. Maldarelli, and K. Strebel. 1996. The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains. J. Virol. 70: 809-819.
22. Schubert, U., P. Henklein, B. Boldyreff, E. Wingender, K. Strebel, and T. Porstmann. 1994. The human immunodeficiency virus type 1 encoded Vpu protein is phosphorylated by casein kinasc-2 (CK-2) at positions Ser52 and Ser56 within a predicted alpha-helix-turn-alpha-helix-motif. J. Mol. Biol. 236:16-25.
23. Schubert, U., T. Schneider, P. Henklein, K. Hoffmann, E. Berthold, H. Hauser, G. Pauli, and T. Porstmann. 1992. Human-immunodeficiency-virus-type-1-encoded Vpu protein is phosphorylated by casein kinase II. Eur. J. Biochem. 204:875-883.
24. Skehel, J. J. 1992. Amantadine blocks the channel. Nature (London) 358: 110-111.
25. Strebel, K., T. Klimkait, F. Maldarelli, and M. A, Martin. 1989. Molecular and biochemical analyses of human immunodeficiency virus type 1 Vpu protein. J. Virol. 63:3784-3791.
26. Strebel, K., T. Klimkait, and M. A. Martin. 1988. A novel gene of HIV-1. vpu, and its 16-kilodalton product. Science 241:1221-1223.
27. Sugrue, R. J., G. Bahadur, M. C. Zambon, S. M. Hall, A. R. Douglas, and A. J. Hay. 1990. Specific structural alteration of the influenza haemagglutinin by amantadine. EMBO J. 9:3469-3476.
28. Sugrue, R. J., and A. J. Hay. 1991. Structural characteristics of the M2 protein of influenza A viruses: evidence that it forms a tetrameric channel. Virology 180:617-624.
29. Sunstrom, N. A., L. S. Premkumar, A. Premkumar, G. Ewart, G. B. Cox, and P. W. Gage. 1996. Ion channels formed by NB, and influenza B virus protein. J. Membr. Biol. 150:127-132.
30. Terwilliger, E. F., E. A. Cohen, Y. C. Lu, J. G. Sodroski, and W. A. Haseltine. 1989. Functional role of human immunodeficiency virus type 1 vpu. Proc. Natl. Acad. Sci. USA 86:5163-5167.
31. Tosteson, M. T., L. H. Pinto, L. J. Holsinger, and R. A. Lamb. 1994. Reconstitution of the influenza virus M2 ion channel in lipid bilayers. J. Membr. Biol. 142:117-126.
32. Trono, D. 1995. HIV accessory proteins: leading roles for the supporting cast. Cell 82:189-192.
33. Varadhachary, A., and P. C. Maloney. 1990. A rapid method for reconstitution of bacterial membrane proteins. Mol. Microbiol. 4:1407-1411.
34. Wang, C., R. A. Lamb, and L. H. Pinto. 1994. Direct measurement of the influenza A virus M2 protein ion channel activity in mammalian cells. Virology 205:133-140.
35. Willey, R. L., F. Maldarelli, M. A. Martin, and K. Strebel. 1992. Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4. J. Virol. 66:7193-7200.
36. Yamato, L, M. Kotani, Y. Oka, and Y. Anraku. 1994. Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli. J. Biol. Chem. 269:5729-5724.