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Volumn 122, Issue 5, 2000, Pages 990-991

Galactose oxidase pro-sequence cleavage and cofactor assembly are self- processing reactions [21]

Author keywords

[No Author keywords available]

Indexed keywords

GALACTOSE OXIDASE;

EID: 0034624436     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja993385y     Document Type: Letter
Times cited : (69)

References (19)
  • 1
    • 12944320622 scopus 로고
    • For a review of posttranslationally modified redox-active amino acids see the following volume: Klinman, J. P., Ed.; Methods Enzymol. 1995, 258.
    • (1995) Methods Enzymol. , vol.258
    • Klinman, J.P.1
  • 11
    • 12944298015 scopus 로고    scopus 로고
    • note
    • All glassware was washed with concentrated nitric/sulphuric acid (1: 1) and the plasticware was soaked in 0.1 M EDTA. Solutions were prepared either by using Chelex-100 resin (BioRad)-treated double deionized water or by using a batch Chelex-100 resin treatment method. Metal-free galactose oxidase was purified from filtered growth media using a batch protein-loading step prior to cellulose phosphate ion exchange chromatography. The protein was eluted with a linear gradient (10 to 100 mM potassium phosphate, pH 7.3). Fractions were screened using both absorbance measurements and SDS-PAGE. Selected fractions were concentrated and exchanged into 20 mM PIPES, pH 6.8, using ultrafiltration. The purification was conducted in the presence of 1 mM EDTA.
  • 12
    • 12944321981 scopus 로고    scopus 로고
    • note
    • Additionally, inductively coupled plasma atomic emission spectroscopy (ICP-AES) of a similar sample showed the copper content to be below the detection limit of the instrument prior to the addition of Cu(II).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.