The role of steric hindrance in 3TC resistance of human immunodeficiency virus type-1 reverse transcriptase

Journal of Molecular Biology

Volumn 300, Issue 2, 2000, Pages 403-418

  Gao, Hong Qiang ^a   Boyer, Paul L ^a   Sarafianos, Stefan G ^b   Arnold, Edward ^b   Hughes, Stephen H ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

3TC; Drug resistance; HIV 1; Reverse transcriptase; RNase H

Indexed keywords

LAMIVUDINE; RNA DIRECTED DNA POLYMERASE;

ANTIBIOTIC RESISTANCE; ARTICLE; COMPLEX FORMATION; DRUG PROTEIN BINDING; ENZYME ACTIVE SITE; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; STEREOSPECIFICITY; VIRUS MUTATION; VIRUS RESISTANCE;

EID: 0034616958     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3823     Document Type: Article

References (29)

1. The processivity of DNA synthesis exhibited by drug-resistant variants of human immunodeficiency virus type-1 reverse transcriptase
2. Analysis of mutations at position 184 in reverse transcriptase of human immunodeficiency virus type 1
3. Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1
4. Subunit specificity of mutations that confer resistance to nonnucleoside inhibitors in human immunodeficiency virus type 1 reverse transcriptase
5. Divalent cation modulation of the ribonuclease functions of human immunodeficiency virus reverse transcriptase
6. Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with double-stranded DNA template-primer and an antibody Fab fragment at 2.8 Å resolution
7. Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution
8. Mechanistic studies examining the efficiency and fidelity of DNA synthesis by the 3TC-resistant mutant (184V) of HIV-1 reverse transcriptase
9. When retroviral reverse transcriptases reach the end of their RNA templates
10. Effects of mutations in the polymerase domain on the polymerase, RNase H and strand transfer activities of human immunodeficiency virus type 1 reverse transcriptase
11. Truncating alpha-helix E' of p66 human immunodeficiency virus reverse transcriptase modulates RNase H function and impairs DNA strand transfer
12. Human immunodeficiency virus type 1 reverse transcriptase: Spatial and temporal relationship between the polymerase and RNase H activities
13. HIV-1 reverse transcriptase-associated RNase H cleaves RNA/RNA in arrested complexes: Implications for the mechanism by which RNase H discriminates between RNA/RNA and RNA/DNA
14. Structure of unliganded human immunodeficiency virus type 1 reverse transcriptase at 2.7 Å resolution: Implications of conformational changes for polymerization and inhibition mechanisms
15. Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: Implications for drug resistance
16. Crystals of a ternary complex of human immunodeficiency virus type 1 reverse transcriptase with a monoclonal antibody Fab fragment and double-stranded DNA diffract X-rays to 3.5-Å resolution
17. Single-step kinetics of HIV-1 reverse transcriptase mutants responsible for virus resistance to nucleoside inhibitors zidovudine and 3TC
18. Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography
19. Subunit-selective mutagenesis indicates minimal polymerase activity in heterodimer-associated p51 HIV-1 reverse transcriptase
20. Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: Structural basis for nucleotide incorporation
21. Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase
22. Dominance of the E89G substitution in HIV-1 reverse transcriptase in regard to increased polymerase processivity and patterns of pausing
23. Vectors for selective expression of cloned DNAs by T7 RNA polymerase
24. 3TC (Lamivudine) resistance in HIV-1 reverse transcriptase involves steric hindrance with β-branched amino acids
25. HIV-1 RT associated ribonuclease H displays both endonuclease and 3' → 5' exonuclease activity
26. Decreased processivity of human immunodeficiency virus type-1 reverse transcriptase (RT) containing didanosine-selected mutation Leu74Val: A comparative analysis of RT variants Leu74Val and lamivudine-selected Met184Val
27. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
28. Nucleotide-induced stable complex formation by HIV-1 reverse transcriptase
29. Human immunodeficiency virus type-1 reverse transcriptase: Contribution of Met-184 to binding of nucleoside 5'-triphosphate