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note
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2- showed no effect). Absorption and far-UV CD were measured on Cary 50 and OLIS instruments, respectively. Thermal denaturation was monitored from 10 to 80°C: CD (205-260 nm) or absorption (250-500 nm) monitored every 5°C. The peptide-Cu complex did not aggregate since absorption and far-UV CD titrations showed linear dependence of complex concentration (10-400 μM), HPLC chromatography revealed similar elution times for peptide alone and peptide-Cu complex (1 mM) and, by electrospray mass spectroscopy, monomeric Cu-peptide species were detected (200 μM).
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