SR proteins ASF/SF2 and 9G8 interact to activate enhancer-dependent intron D splicing of bovine growth hormone pre-mRNA in vitro

RNA

Volumn 6, Issue 12, 2000, Pages 1847-1858

  Li, X ^a   Shambaug, M E ^a   Rottman, F M ^a   Bokar, J A ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

Author keywords

9G8; Alternative splicing; ASF SF2; Bovine growth hormone; Splicing enhancer; SR protein

Indexed keywords

GROWTH HORMONE; MESSENGER RNA; SERINE DERIVATIVE;

ALTERNATIVE RNA SPLICING; ARTICLE; BACULOVIRUS; CONCENTRATION RESPONSE; ENHANCER REGION; HUMAN; HUMAN CELL; INTRON; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; RNA SPLICING; RNA TRANSLATION; VIRUS RECOMBINANT;

ALTERNATIVE SPLICING; ANIMALS; BASE SEQUENCE; BINDING SITES; CATTLE; CONSENSUS SEQUENCE; ENHANCER ELEMENTS (GENETICS); GLOBINS; GROWTH HORMONE; HUMANS; INTRONS; MACROMOLECULAR SUBSTANCES; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; RNA PRECURSORS; RNA-BINDING PROTEINS;

BOVINAE; UNIDENTIFIED BACULOVIRUS;

EID: 0034545535     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838200000674     Document Type: Article

References (52)

1. Exon recognition in vertebrate splicing
2. A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition
3. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers
4. Characterization and cloning of the human splicing factor 9G8: A novel 35 kDa factor of the serine/arginine protein family
5. Directing alternative splicing: Cast and scenarios
6. Identification of a new class of exonic splicing enhancers by in vivo selection
7. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
8. A purine-rich exon sequence enhances alternative splicing of bovine growth hormone pre-mRNA
9. Mapping the SF2/ASF binding sites in the bovine growth hormone exonic splicing enhancer
10. Multiple splicing signals control alternative intron retention of bovine growth hormone pre-mRNA
11. Selection of splice sites in pre-mRNAs with short internal exons
12. The superfamily of arginine/serine-rich factors
13. Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators
14. A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers
15. Alternative processing of bovine growth hormone mRNA is influenced by downstream exon sequences
16. The function of multisite splicing enhancers
17. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing
18. A role for SRp54 during intron bridging of small introns with pyrimidine tracts upstream of the branch point
19. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
20. Functional expression of cloned human splicing factor SF2: Homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators
21. The structure and function of proteins involved in mammalian pre-mRNA splicing
22. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding
23. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
24. An intron enhancer containing a 5'-splice site sequence signals: A Gibbs sampling strategy for multiple alignment
25. Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer
26. SR proteins and splicing control
27. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
28. A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer
29. Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55
30. RNA-RNA interactions in nuclear pre-mRNA splicing
31. Transcriptional activation by recruitment in bacteria and yeast
32. A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer
33. Spliceosome assembly
34. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesical
35. Muscle-specific splicing enhancers regulate inclusion of the cardiac troponin T alternative intron exon in embryonic skeletal muscle
36. Selection and characterization of pre-mRNA splicing enhancers: Identification of novel SR protein-specific enhancer sequences
37. Identification and characterization of three members of the human SR family of pre-mRNA splicing factors
38. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
39. Mechanical devices of the spliceosome: Motors, clocks, springs and things
40. In vivo recognition of a vertebrate mini-exon as an exon-intron-exon unit
41. In vitro analysis of bovine growth hormone pre-mRNA alternative splicing
42. General splicing factor ASF/SF2 promotes alternative splicing by binding to an exonic splicing enhancer
43. Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: Creation of an SRp40-specific splicing enhancer
44. The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities
45. Polypurine sequences within a downstream exon function as a splicing enhancer
46. Selection of novel exon recognition elements from a pool of random sequences
47. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA
48. The role of exon sequences in splice site selection
49. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
50. The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element
51. A complex of nuclear proteins mediates SR protein binding to purine-rich splicing enhancer
52. SR proteins: A conserved family of pre-mRNA splicing factors