Distribution of GPI-anchored proteins in the protozoan parasite Leishmania, based on an improved ultrastructural description using high-pressure frozen cells

Journal of Cell Science

Volumn 113, Issue 24, 2000, Pages 4587-4603

  Weise, F ^a   Stierhof, Y D ^a   Kuhn C ^a   Wiese, M ^a   Overath, P ^a  

^a MAX PLANCK INST FÜR BIOLOGIE   (Germany)

Author keywords

Cis Golgi; Cytoskeleton; Glycosylphosphatidylinositol anchored protein; Golgi structure; High pressure freezing; Immunoelectron microscopy; Leishmania mexicana; Microdomain; Protein sorting; trans Golgi

Indexed keywords

CELL SURFACE PROTEIN; GLYCOSYLPHOSPHATIDYLINOSITOL; MEMBRANE PROTEIN; PROTEINASE;

ARTICLE; BIOTINYLATION; CELL SURFACE; CELL ULTRASTRUCTURE; CELL VACUOLE; CONTROLLED STUDY; DENSITY GRADIENT; ENDOCYTOSIS; ENDOPLASMIC RETICULUM; ENDOSOME; EXOCYTOSIS; FLAGELLUM; FLUORESCENCE ACTIVATED CELL SORTER; FREEZE DRYING; FREEZING; FROZEN SECTION; GOLGI COMPLEX; IMMUNOELECTRON MICROSCOPY; INSECT; LEISHMANIA MEXICANA; LYSOSOME; MICROTUBULE; NONHUMAN; PHYLOGENY; PRESSURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; SPINDLE CELL; TRYPANOSOMA;

INSECTA; LEISHMANIA; LEISHMANIA MEXICANA; PROTOZOA; TRYPANOSOMA; TRYPANOSOMATIDAE;

EID: 0034496239     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (70)

1. Subunit vaccination of mice against New World cutaneous Leishmaniasis: Comparison of three proteins expressed in amastigotes and six adjuvants
2. Protein sorting by directed maturation of Golgi compartments
3. Expression of lipophosphoglycan, high-molecular weight phosphoglycan and glycoprotein 63 in promastigotes and amastigotes of Leishmania mexicana
4. Membrane dynamics at the endoplasmic reticulum-Golgi interface
5. Golgi-mediated post-translational processing of secretory acid phosphatase by Leishmania donovani promastigotes
6. N-glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin-Darby canine kidney cells
7. Acyl and alkyl chain length of GPI-anchors is critical for raft association in vitro
8. Deletion of the gene for the membrane-bound acid phosphatase of Leishmania mexicana
9. The promastigote surface protease of Leishmania
10. The fine structure of Crithidia fasciculata with special reference to the organelles involved in ingestion and digestion of protein
11. Functions of lipid rafts in biological membranes
12. Rapid lateral diffusion of the variant surface glycoprotein in the coat of Trypanosoma brucei
13. VIP17/MAL, a lipid raft-associated protein, is involved in apical transport in MDCK cells
14. Genetic nomenclature for Trypanosoma and Leishmania
15. Protein trafficking in kinetoplastid protozoa
16. Identification, purification and properties of clone specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei
17. A specialized pathway affecting virulence glycoconjugates in Leishmania
18. Intracellular transport of a variant surface glycoprotein in Trypanosoma brucei
19. Endocytosis and intracellular transport of variant surface proteins in trypanosomes
20. The surface glycoconjugates of trypanosomatid parasites
21. The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research
22. Endocytosis and intracellular occurrence of the variant surface glycoprotein in Trypanosoma congolense
23. A unique, terminally glucosylated oligosaccharide is a common feature on Leishmania cell surfaces
24. Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane
25. A quantitative analysis of the endocytic pathway in baby hamster kidney cells
26. The cytoskeleton of trypanosomatid parasites
27. Use of the green fluorescent protein as a marker in transfected Leishmania
28. The endoplasmic reticulum-Golgi intermediate compartment
29. High-pressure freezing of cell suspensions in cellulose capillary tubes
30. Lipophosphoglycan is not required for infection of macrophages or mice by Leishmania mexicana
31. Proteophosphoglycans from Leishmania promastigotes and amastigotes
32. Monoclonal antibodies to Leishmania secreted acid phosphatase and lipophosphoglycan: Partial characterization of private and public epitopes
33. Molecular cloning and characterization of a novel repeat-containing Leishmania major gene, ppg1, that encodes a membrane-associated form of proteophosphoglycan with a putative glycosylphosphatidylinositol anchor
34. Glycosylphosphatidylinositol biosynthetic enzymes are localized to a stable subcompartment of the endoplasmic reticulum in Leishmania mexicana
35. Lipophosphoglycan masks recognition of the Leishmania donovani promastigote surface by human kala-azar serum
36. Adaptors for clathrin-mediated traffic
37. Membrane microdomains and caveolae
38. Golgi GDP-mannose uptake requires Leishmania LPG2. A member of a eucaryotic family of putative nucleotide-sugar transporters
39. Cholesterol-dependent retention of GPI-anchored proteins in endosomes
40. The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes
41. The promastigote surface protease GP63 of Leishmania is expressed but differentially processed and localized in the amastigote stage
42. Purification and characterization of a membrane-bound acid phosphatase of Leishmania mexicana
43. Theory and practice of high pressure freezing
44. Endocytosis
45. Visualization of the dynamics of synaptic vesicle and plasma membrane proteins in living axons
46. Structures of the N-linked oligosaccharides of GP63, the major surface glycoprotein from Leishmania mexicana amazonensis
47. Glycophingolipids are not essential for formation of detergent-resistant membrane rafts in melanoma cells
48. Endocytosis and secretion in trypanosomatid parasites - Tumultuous traffic in a pocket
49. The comparative fine structure and surface glycoconjugate expression of three life stages of Leishmania major
50. The form and function of the cytostome-cytopharynx of the culture form of the Elasmobranch haemoflagellate Trypanosoma raiae Laveran & Mesnil
51. Endocytosis of GPI-linked membrane folate receptor-α
52. The crystal structure of the Leishmania major surface proteinase leishmanolysin (GP63)
53. Structure of the glycosylphosphatidylinositol membrane anchor of the Leishmania major promastigote surface protease
54. Functional rafts in cell membranes
55. A new method of preparing gold probes for multiple-labeling cytochemistry
56. Phylogenetic meaning of the kingdom concept: An unusual ribosomal RNA from Gardia lamblia
57. Freeze-substitution and freeze-drying
58. The existence of a cytostome and the occurrence of pinocytosis in the trypanosome, Trypanosoma mega
59. Transferrin-binding protein complex is the receptor for transferrin uptake in Trypanosoma brucei
60. Labeling properties of sucrose-infiltrated cryosections
61. Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors
62. Characterization of polymer release from the flagellar pocket of Leishmania mexicana promastigotes
63. High pressure freezing comes of age
64. Dual-color visualization of trans-Golgi network to plasma membrane traffic along microtubules in living cells
65. Intracellular colocalization of variant surface glycoprotein and transferrin-gold in Trypanosoma brucei (1988)
66. The flagellar pocket of trypanosomatids
67. Novel entry pathway of bovine herpesvirus 1 and 5
68. Gene cloning and cellular localization of a membrane-bound acid phosphatase of Leishmania mexicana