Cellular activities of 20K- and 22K-hGH do not necessarily correlate with their binding affinities for rat GH receptor

Hormone Research

Volumn 54, Issue 3, 2000, Pages 136-142

  Ikeda, M ^a   Matsumoto, K ^a   Uchida, H ^a   Naito, N ^a   Tsunekawa, B ^a   Wada, Mitsufumi ^a   Honjo, M ^a  

^a MITSUI CHEMICALS INC   (Japan)

Author keywords

20K hGH W22K hGH; Binding affinity; Cell proliferation; Gene promoter activation; Rat GH receptor

Indexed keywords

COMPLEMENTARY DNA; GROWTH HORMONE; GROWTH HORMONE RECEPTOR;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CELL PROLIFERATION; COMPLEX FORMATION; CONTROLLED STUDY; GEL FILTRATION; GENE ACTIVATION; GENE EXPRESSION; HORMONE ACTION; HORMONE RECEPTOR INTERACTION; HYPOPHYSECTOMY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; WEIGHT GAIN;

ADIPOSE TISSUE; ANIMALS; CELL DIVISION; CELL LINE; CHO CELLS; CHROMATOGRAPHY, GEL; CRICETINAE; GENE EXPRESSION; HUMAN GROWTH HORMONE; HUMANS; HYPOPHYSECTOMY; MICROSOMES; MICROSOMES, LIVER; MOLECULAR WEIGHT; NUCLEAR PROTEINS; PEPTIDE FRAGMENTS; PROMOTER REGIONS (GENETICS); RATS; RECEPTORS, SOMATOTROPIN; STRUCTURE-ACTIVITY RELATIONSHIP; TRANSFECTION;

EID: 0034444761     PISSN: 03010163     EISSN: None     Source Type: Journal    
DOI: 10.1159/000053247     Document Type: Article

References (31)

1. DeNoto FM, Moore DD, Goodman HM: Human growth hormone DNA sequence and mRNA structure: Possible alternative splicing. Nucleic Acids Res 1981;9:3719-3730.
2. Sigel MB, Thorpe NA, Kobrin MS, Lewis U, Vanderlaan WP: Binding characteristics of a biologically active variant of human growth hormone (20K) to growth hormone and lactogen receptors. Endocrinology 1981;108:1600-1603.
3. Wohnlich M, Moore WV: Binding of a variant of human growth hormone to liver plasma membranes. Horm Metabol Res 1982;14:138-141.
4. Barnard R, Rowlinson SW, Waters MJ: Soluble forms of the rabbit tissue and liver growth hormone receptors are antigenically identical, but the integral membrane forms differ. Biochem J 1990;267:471-477.
5. Ohmae Y, Yano H, Umezawa S, Tanaka T, Hibi I, Miyamoto C, Furuichi Y: Biological activities of synthesized 20K and 22K hGH in Nb2 bioassay and IM-9 radioreceptor assay. Endocrinol Jpn 1989;36:9-13.
6. Dattani MT, Hindmarsh PC, Brook CGD, Robinson ICAF, Marshall NJ: Inhibition of growth hormone bioactivity by recombinant human growth hormone-binding protein in the eluted stain assay system. J Endocrinol 1994; 140:445-453.
7. Lewis UJ, Dunn JT, Bonewald LF, Seavey BK, Vanderlaan WP: A naturally occurring structural variant of human growth hormone. J Biol Chem 1978;253:2679-2687.
8. Spencer EM, Lewis LJ, Lewis UJ: Somatomedin generating activity of the 20,000-dalton variant of human growth hormone. Endocrinology 1981;109:1301-1302.
9. Nishikawa S, Nishida Y, Uemura H, Yamada Y, Tanaka T, Uesugi S, Morikawa M, Uchida E, Hayakawa T, Ikehara M: Structure and activity of artificial mutant variants of human growth hormone. Protein Eng 1989;3:49-53.
10. Fuh G, Cunningham BC, Fukunaga R, Nagata S, Goeddel DV, Wells JA: Rational design of potent antagonists to the human growth hormone receptor. Science 1992;256:1677-1680.
11. Uchida H, Naito N, Asada N, Wada M, Ikeda M, Kobayashi H, Asanagi M, Mori K, Fujita Y, Konda K, Kusuhara N, Kamioka T, Nakashima K, Honjo M: Secretion of authentic 20-kDa human growth hormone (20K-hGH) in Escherichia coli and properties of the purified product. J Biotechnol 1997;55:101-112.
12. Wada M, Ikeda M, Takahashi Y, Asada N, Chang KT, Takahashi M, Honjo M: The full effect of recombinant 20 kDa human growth hormone (hGH) on CHO cells stably transfected with hGH receptor cDNA. Mol Cell Endocrinol 1997;133:99-107.
13. Mathews LS, Enberg B. Norstedt G: Regulation of rat growth hormone receptor gene expression. J Biol Chem 1989;264:9905-9910.
14. Wada M, Uchida H, Ikeda M, Tsunekawa B, Naito N, Banba S, Tanaka E, Hashimoto Y, Honjo M: The 20-kilodalton (kDa) human growth hormone differs from the 22-kDa hGH in the complex formation with cell surface hGH receptor and hGH-binding protein circulating in human plasma. Mol Encodrinol 1998; 12:146-156.
15. Roswall EC, Mukku VR, Chen AB, Hoff EH, Chu H, McKay PA. Olson KC, Battersby JE. Gehant RL, Meunier A, Garnick RL: Novel assays based on human growth hormone receptor as alternatives to the rat weight gain bioassay for recombinant human growth hormone. Biologicals 1996;24:25-39.
16. Cunningham BC, Ultsch M, De Vos AM, Mulkerrin MG, Clauser KR, Wells JA: Dimerization of the extracellular domain of the human growth hormone receptor by a single hormone molecule. Science 1991;254:821-825.
17. Yoon JB, Berry SA, Seelig S, Towle HC: An inducible nuclear factor binds to a growth hormone-regulated gene. J Biol Chem 1990;265: 19947-19954.
18. Baumann G, Stolar MW, Buchanan TA: Slow clearance rate of the 20,000-dalton variant of human growth hormone: Implications for biological activity. Endocrinology 1985;117: 1309-1313.
19. Somer W, Ultsch M, De Vos AM, Kossiakoff AA: The X-ray structure of a growth hormone-prolactin receptor complex. Nature 1994;372: 478-481.
20. Gertler A, Bignon C, Staten NR, Sakal E, Tehelet A, Krivi GG, Djiane J: Interaction of lactogenic hormones with the soluble extracellular domain of prolactin receptors. Proc Soc Exp Biol Med 1994;206:273-279.
21. Bignon C, Sakal E, Belair L, Chapnik-Cohen N, Djiane J, Gertler A: Preparation of the extracellular domain of the rabbit prolaetin receptor expressed in Escherichia coli and its interaction with lactogenic hormones. J Biol Chem 1994; 269:3318-3324.
22. Fuh G, Colosi P, Wood WI, Wells JA: Mechanism-based design of prolactin antagonists. J Biol Chem 1993;268:5376-5381.
23. Gertler A, Grosclaude J, Strasburger CJ, Nir S, Djiane J: Real-time kinetic measurements of the interactions between lactogenic hormones and prolatin-receptor extracellular domains from several species support the model of hormone-induced transient receptor dimerization. J Biol Chem 1996;271:24482-24491.
24. De Vos AM, Ultsch M, Kossiakoff AA: Human growth hormone and extracellular domain of its receptor: Crystal structure of the complex. Science 1992;255:306-312.
25. Nagatomi Y, Ikeda M, Uchida H, Wada M, Kobayashi H, Hashimoto Y, Mabuchi K, Hayakawa M, Kusuhara N, Honjo M: Reversible dimerization of 20-kilodalton human growth hormone. Growth Horm IGF Res 2000; 10:207-214.
26. Kostyo JL, Cameron CM, Olson KC, Jones AJ, Pai RC: Biosynthetic 20-kilodalton methionyl-human growth hormone has diabetogenic and insulin-like activities. Proc Natl Acad Sci USA 1985;82:4250-4253.
27. Smal J, Closset J, Hennen G, DeMeyts P: Receptor binding properties and insulin-like effects of human growth hormone and its 20-kDa variant in rat adipocytes. J Biol Chem 1987;262:11071-11079.
28. Cameron CM, Kostyo JL, Adamafio NA, Brostedt P, Roos P, Skottner A, Frosman A, Fryklund L, Skoog B: The acute effects of growth hormone on amino acid transport and protein synthesis are due to its insulin-like action. Endocrinology 1988;122:471-474.
29. Tsunekawa B, Wada M, Ikeda M, Uchida H, Naito N, Honjo M: The 20-kilodalton (kDa) human growth hormone (hGH) differs from the 22-kDa hGH in the effect on the human prolactin receptor. Endocrinology 1999;140: 3909-3918.
30. Wang YD, Wood WI: Amino acids of the human growth hormone receptor that are required for proliferation and Jak-STAT signaling. Mol Endocrinol 1995;9:303-311.
31. Wood TJJ, Sliva D, Lobie PE, Pircher T, Gouilleux F, Wakao H, Gustafsson JÅ. Groner B, Norstedt G, Haldosén LA: Mediation of growth hormone-dependent transcriptional activation by mammary gland factor/Stat 5. J Biol Chem 1995;270:9448-9453.