Mutational analysis of the yeast DEAH-box splicing factor Prp16

Genetics

Volumn 149, Issue 2, 1998, Pages 807-815

  Hotz, Hans Rudolf ^a   Schwer, Beate ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HOMEODOMAIN PROTEIN; NUCLEOSIDE TRIPHOSPHATASE; SPLICING FACTOR PRP 16; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; DELETION MUTANT; LETHAL MUTANT; NONHUMAN; PRIORITY JOURNAL; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SUBSTITUTION; CELL DIVISION; FUNGAL PROTEINS; GENES, DOMINANT; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POINT MUTATION; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE DELETION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0031800746     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. BEGGS, J. D., 1995 Yeast splicing factors and genetic stratagies for their analysis, pp. 79-95 in Pre-mRNA Processing, edited by A. I. LAMOND. R. G. Landes Company, Austin, TX.
2. BOEKE, J. D., J. TRUEHEART, G. NATSOULIS and G. R. FINK, 1987 5-Fluorootic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154: 164-175.
3. BURGESS, S., J. R. COUTO and C. GUTHRIE, 1990 A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell 60: 705-717.
4. CHEN, J.-H., and R.-J. LIN, 1990 The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors. Nucleic Acids Res. 18: 6447.
5. COMPANY, M., J. ARENAS and J. ABELSON, 1991 Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from the spliceosomes. Nature 349: 487-493.
6. FERNÁNDEZ, A., S. LAÍN and J. A. GARCÍA, 1995 RNA helicase activity of the plum pox potyvirus C1 protein expressed in Escherichia coli. Mapping of an RNA binding domain. Nucleic Acids Res. 23: 1327-1332.
7. GORBALENYA, A. E., E. V. KOONIN, A. P. DONCHENKO and V. M. BLINOV, 1989 Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 17: 4713-4730.
8. GROSS, C. H., and S. SHUMAN, 1995 Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase. J. Virol. 69: 4727-4736.
9. GROSS, C. H., and S. SHUMAN, 1996 The QRxGRxGRxxxG motif of the vaccinia virus DExH box RNA helicase NPH-II is required for ATP hydrolysis and RNA unwinding but not for RNA binding. J. Virol. 70: 1706-1713.
10. HEILEK, G. M., and M. G. PETERSON, 1997 A point mutation abolishes the helicase but not the nucleoside triphosphatase activity of hepatitis C virus NS3 protein. J. Virol. 71: 6264-6266.
11. JIN, L., and D. L. PETERSON, 1995 Expression, isolation, and characterization of the hepatitis C virus ATPase/RNA helicase. Arch. Biochem. Biophys. 323: 47-53.
12. KIM, S. H., and R.-J. LIN, 1993 Pre-mRNA splicing within an assembled yeast spliceosome requires an RNA-dependent ATPase and ATP hydrolysis. Proc. Natl. Acad. Sci. USA 90: 888-892.
13. KIM, S. H., and R.-J. LIN, 1996 Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol. Cell. Biol. 16: 6810-6819.
14. KIM, S. H., J. SMITH, A. CLAUDE and R.-J. LIN, 1992 The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. EMBO J. 11:2319-2326.
15. KIM, D. W., Y. GWACK, J. H. HAN and J. CHOE, 1995 C-terminal domain of the hepatitis C virus NS3 protein contains an RNA helicase activity. Biochem. Biophys. Res. Commun. 215: 160-166.
16. KING, D. S., and J. D. BEGGS, 1990 Interaction of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae. Nucleic Acids Res. 18: 6559-6564.
17. KOROLEV, S., J. HSIEH, G. H. GAUSS, T. M. LOHMAN and G. WAKSMAN, 1997 Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP. Cell 90: 635-647.
18. LEE, C. G., and J. HURWITZ, 1993 Human RNA helicase A is homologous to the maleless protein of Drosophila. J. Biol. Chem. 268: 16822-16830.
19. LEE, C. G., K. A. CHANG, M. I. KURODA and J. HURWITZ, 1997 The NTPase/helicase activities of Drosophila maleless, an essential factor in dosage compensation. EMBO J. 16: 2671-2681.
20. LINDER, P., P. F. LASKO, M. ASHBURNER, P. LEROY, P. J. NIELSEN et al., 1989 Birth of the D-E-A-D box. Nature 377: 121-122.
21. MADHANI, H. D., and C. GUTHRIE, 1994 Dynamic RNA-RNA interactions in the spliceosome. Annu. Rev. Genet. 28: 1-26.
22. MOORE, M. J., C. C. QUERY and P. A. SHARP, 1993 Splicing of precursors to mRNA by the spliceosome, pp. 303-357 in The RNA World, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. NILSEN, T. W., 1994 RNA-RNA interactions in the spliceosome: unraveling the ties that bind. Cell 78: 1-4.
24. OHNO, M., and Y. SHIMURA, 1996 A human RNA helicase-like protein, HRH1, facilitates export of spliced mRNA by releasing the RNA from the spliceosome. Genes Dev. 10: 997-1007.
25. PAI, E. F., U. KRENGEL, G. A. PETSKO, R. S. GOODY, W. KABSCH et al., 1990 Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 Å resolution: implication for the mechanism of GTP hydrolysis. EMBO J. 9: 2351-2359.
26. PAUSE, A., and N. SONENBERG, 1992 Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A. EMBO J. 11: 2643-2654.
27. PAUSE, A., N. MÉTHOT and N. SONENBERG, 1993 The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis. Mol. Cell. Biol. 13: 6789-6798.
28. PLUMPTON, M., M. MCGARVEY and J. D. BEGGS, 1994 A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes. EMBO J. 13: 879-887.
29. RAY, B. K., T. G. LAWSON, J. C. KRAMER, M. H. CLADARAS, J. A. GRIFO et al., 1985 ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors. J. Biol. Chem. 260:7651-7658.
30. ROZEN, F., J. PELLETIER, H. TRACHSEL and N. SONENBERG, 1989 A lysine substitution in the ATP-binding site of eucaryotic initiation factor 4A abrogates nucleotide-binding activity. Mol. Cell. Biol. 9: 4061-4063.
31. RUBY, S. W., and J. ABELSON, 1991 Pre-mRNA splicing in yeast. Trends Genet. 7: 79-85.
32. SCHMID, S. R., and P. LINDER, 1991 Translation initiation factor 4A from Saccharomyces cerevisiae: analysis of residues conserved in the D-E-A-D family of RNA helicases. Mol. Cell. Biol. 11: 3463-3471.
33. SCHWER, B., and C. GUTHRIE, 1991 PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349: 494-499.
34. SCHWER, B., and C. GUTHRIE, 1992a A conformational rearrangement in the spliceosome is dependent on the PRP16 and ATP hydrolysis. EMBO J. 11: 5033-5039.
35. SCHWER, B., and C. GUTHRIE, 1992b A dominant negative mutation in a spliceosomal ATPase affects hydrolysis but not binding to the spliceosome. Mol. Cell. Biol. 12: 3540-3547.
36. SCHWER, B., and C. H. GROSS, 1998 Prp22, a DEXH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J. 17: 2086-2094.
37. SHUMAN, S., 1992 Vaccinia virus RNA helicase: an essential enzyme related to the DE-H family of RNA-dependent NTPases. Proc. Natl. Acad. Sci. USA 89: 10935-10939.
38. STORY, R. M., and T. A. STEITZ, 1992 Structure of the recA protein-ADP complex. Nature 349: 374-376.
39. SUBRAMANYA, H. S., L. E. BIRD, J. A. BRANNIGAN and D. B. WIGLEY, 1996 Crystal structure of DExx box DNA helicase. Nature 384: 379-383.
40. YAO, N., T. HESSON, M. CABLE, Z. HONG, A. D. KWONG et al., 1997 Structure of the hepatitis C virus RNA helicase domain. Nat. Struct. Biol. 4: 463-467.