Preparation of spatially ordered multilayer thin films of antibody and their binding properties

Biosensors and Bioelectronics

Volumn 15, Issue 11-12, 2000, Pages 623-628

  Hoshi, T ^a   Saiki, H ^a   Anzai, J I ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Antibody; Avidin; Biotin; Layer by layer deposition; Multilayer thin film

Indexed keywords

ANTIBODY; ANTIGEN; AVIDIN; BIOTIN; FLUORESCEIN ISOTHIOCYANATE;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CHEMICAL STRUCTURE; MICROFILM; ULTRAVIOLET SPECTROPHOTOMETRY;

ANTIGEN-ANTIBODY REACTIONS; BIOSENSING TECHNIQUES; FLUORESCEIN-5-ISOTHIOCYANATE; SPECTROPHOTOMETRY, ULTRAVIOLET;

ANTIGENS; BIOFILMS; DEPOSITION; MULTILAYERS; QUARTZ; SPECTROPHOTOMETRY; THIN FILMS;

AVIDIN; BIOTIN; LAYER-BY-LAYER DEPOSITION;

ANTIBODIES;

EID: 0034351358     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(00)00113-5     Document Type: Article

References (19)

1. Layer-by-layer construction of multilayer thin films composed of avidin and biotin-labeled poly(amine)s
2. Piezoelectric immunosensors for urine specimens of Chlamydia trachomatis employing quartz crystal microbalance microgravimetric analyses
3. Photochemically-activated electrodes: Application in design of reversible immunosensors and antibody patterned interfaces
4. Step-by-step immunological construction of a fully active multilayer enzyme electrode
5. Immobilisation of multilayer bioreceptor assemblies on solid substrates
6. 2. Assembly of alternating polyelectrolyte and protein multilayer films for immunosensing
7. Amperometric alcohol sensors based on protein multilayers composed of avidin and biotin-labeled alcohol oxidase
8. Enzyme coupled lactose sensors based on bienzyme membrane prepared by an avidin/biotin method
9. Layer-by-layer deposition of avidin and biotin-labeled antibody on a solid surface to prepare a multilayer array of antibody
10. Amperometric amplification of antigen-antibody association at monolayer interfaces: Design of immunosensor electrodes
11. Preparation and amperometric glucose sensitivity of covalently bound glucose oxidase to (2-aminoethyl)ferrocene on an Au electrode
12. Functionalization of gold surfaces for specific and reversible attachment of a fused β-galactosidase and choline-receptor protein
13. Protein A Langmuir-Blodgett film for antibody immobilization and its use in optical immunosensing
14. An immunosensor based on the glucose oxidase label and optical oxygen detection
15. Amplified microgravimetric quartz-crystal-microbalance assay of DNA using oligonucleotide functionalized liposomes or biotinylated liposomes
16. Glucose and acetylcholine sensing multilayer enzyme electrodes of controlled enzyme layer thickness
17. Enzyme immunoassays with an electrochemical detection method using alkaline phosphate and a perfluorosulfonated ionomer-modified electrode. Application to phenytoin assays
18. The avidin-biotin complex in bioanalytical applications