Amplified microgravimetric quartz-crystal-microbalance assay of DNA using oligonucleotide-functionalized liposomes or biotinylated liposomes

Journal of the American Chemical Society

Volumn 122, Issue 2, 2000, Pages 418-419

  Patolsky, Fernando ^a   Lichtenstein, Amir ^a   Willner, Itamar ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOSOME; SILICON DIOXIDE;

ARTICLE; BIOTINYLATION; CATALYSIS; CRYSTAL STRUCTURE; DNA DETERMINATION; DNA PROTEIN COMPLEX; DRUG SYNTHESIS; GRAVIMETRY; PROTEIN DNA INTERACTION;

EID: 0033964539     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja992834r     Document Type: Article

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18. -2 by following the frequency changes of the crystal upon modification.
19. -1) at a ratio of 79:20:1:0.1. The liposomes were modified with 3 (4°C, 20 h) and were purified by gel chromatography (Sephadex G-75). The surface coverage of liposome with 3 (50-60 oligonucleotide units per liposome) was determined by reacting the resulting liposomes with OliGreen (Molecular probes) and following the fluorescence intensity of the resulting liposome assay. The size of the liposomes was determined by dynamic light-scattering to be 220 ± 20 nm.
20. -1), and biotinylated phosphatidylethanolamine with a ratio corresponding to 80:20:0.1:0.5. The average coverage of each liposome with biotin units corresponds to 550. The biotin-labeled liposomes were purified by gel chromatography (Sephadex A-25). The size of the liposomes was determined by dynamic light scattering and corresponds to 180 ± 40 nm.