Hybridization of DNA by sequential immobilization of oligonucleotides at the air-water interface

Langmuir

Volumn 16, Issue 24, 2000, Pages 9142-9146

  Sastry, Murali ^a   Ramakrishnan, Vidya ^a   Pattarkine, Mrunalini ^a   Gole, Anand ^a   Ganesh, K N ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEXATION; DNA; FOURIER TRANSFORM INFRARED SPECTROSCOPY; GRAVIMETRIC ANALYSIS; HYDROGEN BONDS; INTERCALATION COMPOUNDS; INTERFACES (MATERIALS); ISOTHERMS; LANGMUIR BLODGETT FILMS; MONOLAYERS; ULTRAVIOLET SPECTROSCOPY;

FLUORESCENCE SPECTROSCOPY; HYBRIDIZATION; OLIGONUCLEOTIDES; QUARTZ CRYSTAL MICROGRAVIMETRY (QCM);

OLIGOMERS;

EID: 0034318482     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la000753z     Document Type: Article

References (37)

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30. 2 (expansion by ∼32%) may be accounted for if one assumes that roughly five out of the eight sites per DNA molecule immobilized at the air-water interface are intercalated by ethidium bromide molecules. This would lead to an increase in length of the DNA molecules by 17 Å and works out to a ∼31% increase in the length of the DNA molecule (normal length ∼ 55 Å). This result is consistent with further uptake of ethidium bromide indicated in the fluorescence measurements shown in Figure 2B.
31. 2 (expansion by ∼32%) may be accounted for if one assumes that roughly five out of the eight sites per DNA molecule immobilized at the air-water interface are intercalated by ethidium bromide molecules. This would lead to an increase in length of the DNA molecules by 17 Å and works out to a ∼31% increase in the length of the DNA molecule (normal length ∼ 55 Å). This result is consistent with further uptake of ethidium bromide indicated in the fluorescence measurements shown in Figure 2B.
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