5′ CpG island methylation of p16 is associated with absence of p16 expression in glioblastomas

Journal of Korean Medical Science

Volumn 15, Issue 5, 2000, Pages 555-559

  Park, Sung Hye ^b   Jung, Kyeong Cheon ^a   Ro, Jae Y ^a   Kang, Gyeong Hoon ^a   Khang, Shin Kwang ^a  

^a University of Ulsan College of Medicine   (South Korea)

^b Inje University   (South Korea)

Author keywords

Glioblostoma; Hypermethylation; Immunohistochemistry; Methylation Specific PCR Study; p16

Indexed keywords

PROTEIN P16INK4A;

5' UNTRANSLATED REGION; ADULT; ARTICLE; BRAIN TUMOR; CHEMISTRY; CPG ISLAND; DNA METHYLATION; FEMALE; GENE SILENCING; GENETICS; GLIOBLASTOMA; HUMAN; MALE; METABOLISM; MIDDLE AGED; NUCLEIC ACID PROBE; PATHOLOGY; PHYSIOLOGY; POLYMERASE CHAIN REACTION;

5' UNTRANSLATED REGIONS; ADULT; ANTISENSE ELEMENTS (GENETICS); BRAIN NEOPLASMS; CPG ISLANDS; CYCLIN-DEPENDENT KINASE INHIBITOR P16; DNA METHYLATION; FEMALE; GENE SILENCING; GLIOBLASTOMA; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION;

EID: 0034302869     PISSN: 10118934     EISSN: None     Source Type: Journal    
DOI: 10.3346/jkms.2000.15.5.555     Document Type: Article

References (21)

1. Serrano M, Hannon GJ, Beach D. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature 1993; 366: 704-7.
2. Kamb A, Gruis NA, Liu Q, Harshman K, Tavtigian SV, Stockert E, Day RS 3rd, Johnson BE, Skolnick MH. A cell cycle regulator potentially involved in genesis of many tumor types. Science 1994; 264: 436-40.
3. Nobori T, Miura K, Wu DJ, Lois A, Takabayashi K, Carson DA. Deletions of the cyclin-dependent kinase-4 inhibitor gene in muptiple human cancers. Nature 1994; 368: 753-6.
4. Merlo A, Herman JG, Mao L, Lee DJ, Gabrielson E, Burger PC, Baylin SB, Sidransky D. 5′CpG island methylation is associated with transcriptional silencing of the tumor suppressor p16/CDK2/MTS1 in human cancers. Nat Med 1995; 1: 686-92.
5. Mori R, Miura K, Aoki T, Nishihira T, Mori S, Nakamura Y. Frequent somatic mutation of the MTS 1/CDK4I (multiple tumor suppressor/cyclin-dependent kinase 4 inhibitor) gene in esophageal squamous cell carcinoma. Cancer Res 1994; 54: 3396-7.
6. INK4A genes in astrocytomas. Diagn Mol Pathol 1997; 6: 115-22.
7. Ueki K, Ono Y, Henson JW, Efird JT, Von Diemling A, Louis DN. CDKN2/p16 or RB alteration occur in the majority of glioblastomas and are inversely correlated. Cancer Res 1996; 56: 150-3.
8. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996; 93: 9821-6.
9. Maesawa C, Tamura G, Nishizuka S, Ogasawara S, Ishida K, Terashima M, Sakata K, Sato N, Saito K, Satodate R. Inactivation of the CDKN2 gene by homozygous deletion and de novo methylation is associated with advanced stage esophageal squamous cell carcinoma. Cancer Res 1996; 56: 3875-8.
10. Reed AL, Califano J, Cairns P, Westra WH, Jones RM, Koch W, Ahrendt S, Eby Y, Sewell D, Nawroz H, Bartek J, Sidransky D. High frequency of p16 (CDKN2/MTS - 1.INK4A) inactivation in head and neck squamous cell carcinoma. Cancer Res 1996; 56: 3630-3.
11. Sonoda Y, Yoshimoto T, Sekiya T. Homozygous deletion of the MTS1/p16 and MTS2/p15 genes and amplification of CDK4 gene in glioma. Oncogene 1995; 11: 2145-9.
12. (INK4) products in primary gastric cancer. Oncology 2000; 58: 126-36.
13. INK4A genes in astrocytomas. Diagn Mol Pathol 1997; 6: 115-22.
14. Sato K, Schauble B, Kleihues P, Ohgaki H. Infrequent alterations of the p15, p16, CDK4 and cyclin D1 genes in non-astrocytic human tumors. Int J Cancer 1996; 66: 305-8.
15. Nakamura M, Konioshi N, Hiasa Y, Tsunoda S, Fukushima Y, Tsuzuki T, Takemura K, Aoki H, Kobitsu K, Sakaki T. Immunohistochemical detection of CDKN2, retinoblastoma and p53 gene products in primary astrocytic tumors. Int J Oncol 1996; 8: 889-93.
16. Louis DN. A molecular genetic model of astrocytoma histopathology. Brain Pathol 1997; 7: 755-64.
17. Lang FF, Miller DC, Koslow M, Newcomb EW. Pathways leading to glioblastoma multiforme: a molecular analysis of genetic alterations in 65 astrocytic tumors. J Neurosurg 1994; 81: 427-36.
18. Ohgaki H, Schauble B, zur Hausen A, von Ammon K, Kleihues P. Genetic alterations associated with the evolution and progression of astrocytic brain tumors. Virchows Arch 1995; 427: 113-8.
19. Cheng Y, Ng HK, Ding M, Zhang SF, Pang JC, Lo KW. Molecular analysis of microdissected de novo glioblastomas and paired astrocytic tumors. J Neuropathol Exp Neurol 1999; 58: 120-8.
20. INK4A expression is frequent in high grade gliomas. Cancer Res 1995; 55: 1941-5.
21. Costello JF, Berger MS, Huang HS, Cavenee WK. Silencing of p16/CDKN2 expression in human gliomas by methylation and chromatin condensation. Cancer Res 1996; 56: 2405-10.