High-density mutagenesis by combined DNA shuffling and phage display to assign essential amino acid residues in protein-protein interactions: Application to study structure-function of plasminogen activation inhibitor 1 (PAI-1)

Journal of Molecular Biology

Volumn 301, Issue 5, 2000, Pages 1135-1147

  Stoop, A Allart ^a   Jespers, Laurent ^b   Lasters, Ignace ^c   Eldering, Eric ^a   Pannekoek, Hans ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b MEDICAL RESEARCH COUNCIL   (United Kingdom)

^c Algonomics   (Belgium)

Author keywords

DNA shuffling; Epitope mapping; PAI 1; Phage display; Protein protein interactions

Indexed keywords

AMINO ACID; DNA; EPITOPE; PLASMINOGEN ACTIVATOR INHIBITOR 1;

AMINO ACID SUBSTITUTION; ARTICLE; EPITOPE MAPPING; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS;

EID: 0034283566     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4035     Document Type: Article

References (42)

1. Assembly of combinatorial antibody libraries on phage surfaces: The gene III site
2. Molecular evolution of plasminogen activator inhibitor-1 functional stability
3. Epitopes on PAI-1 important for binding to t-PA
4. Protein movement during complex formation between t-PA and PAI-1
5. Molecular evolution of an arsenate detoxification pathway by DNA shuffling
6. Neutralization of plasminogen activator inhibitor-1 inhibitory properties: Identification of two different mechanisms
7. Identification of a functional epitope in plasminogen activator inhibitor-1, not localized in the reactive center loop
8. Interactive computer animation of macromolecules
9. Structure-function relationships in serpins: Current concepts and controversies
10. A general method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interactions
11. Implications of the three dimensional structure of α1-antitrypsin for structure and function of serpins
12. The structure of protein-protein recognition sites
13. Epitope mapping by negative selection of randomized antigen libraries displayed on filamentous phage
14. High resolution functional analysis of antibody-antigen interactions
15. Principles of protein-protein interactions
16. The interaction of plasminogen activator inhibitor 1 with plasminogen activators (tissue-type and urokinase-type) and fibrin: Localization of interaction sites and physiologic relevance
17. Principles and applications of phage display
18. Serpin-protease complexes are trapped as stable acyl-enzyme intermediates
19. Regulation of cell adhesion by PAI-1
20. Restoration of the serine protease-inhibitor interaction by protein engineering
21. Interior and surface of monomeric proteins
22. Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of improved sequences
23. Structural basis of latency in plasminogen activator inhibitor-1
24. Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning
25. Phage-display technology - Finding a needle in a vast molecular haystack
26. The active conformation of plasminogen activator inhibitor 1, a target for drugs to control fibrinolysis and cell adhesion
27. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
28. Rapid evolution of a protein in vitro by DNA shuffling
29. Formation of the covalent serpin-proteinase complex involves translocation of the proteinase by more than 70 Å and full insertion of the reactive center loop into β-sheet A
30. Engineering of PAI-1 to reduce the rate of latency transition
31. Structure of PAI-1 and its function in fibrinolysis: An update
32. Selective screening of a large phage display library of plasminogen activator inhibitor 1 mutants to localize interaction sites with either thrombin or the variable region 1 of tissue-type plasminogen activator
33. The suicide substrate reaction between plasminogen activator inhibitor 1 and thrombin is regulated by the cofactors vitronectin and heparin
34. Mapping of epitopes on human tissue-type plasminogen activator with recombinant deletion mutant proteins
35. Identification of functional interaction sites on proteins using bacteriophage-displayed random epitope libraries
36. Accelerated conversion of human plasminogen activator inhibitor-1 to its latent form by antibody binding
37. Systematic mutational analyses of protein-protein interfaces
38. The inhibition mechanism of serpins. Evidence that the mobile reactive center loop is cleaved in the native protease-inhibitor complex
39. Structural insights into serpin-protease complexes reveal the inhibitory mechanism of serpins
40. Structural basis for serpin inhibitor activity
41. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening
42. Optimization of DNA shuffling for high fidelity recombination