Shear Stress Differentially Regulates PGHS-1 and PGHS-2 Protein Levels in Human Endothelial Cells

Annals of Biomedical Engineering

Volumn 28, Issue 7, 2000, Pages 824-833

  McCormick, Susan M ^a   Whitson, Peggy A ^b   Wu, Kenneth K ^c   McIntire, Larry V ^a  

^a Rice University   (United States)

^b NASA JOHNSON SPACE CENTER   (United States)

^c UNIVERSITY OF TEXAS   (United States)

Author keywords

Cyclooxygenase; Endothelium; Gene regulation; Prostaglandin H synthase

Indexed keywords

PROSTACYCLIN; PROSTAGLANDIN SYNTHASE;

ARTICLE; BLOOD RHEOLOGY; CELL CULTURE; CHEMISTRY; CYTOLOGY; GENE EXPRESSION REGULATION; HOMEOSTASIS; HUMAN; ISCHEMIC PRECONDITIONING; MECHANICAL STRESS; METHODOLOGY; NON-PROGRAMMATIC; PHYSIOLOGY; SECRETION; TIME; UMBILICAL CORD; VASCULAR ENDOTHELIUM; WESTERN BLOTTING;

NON-PROGRAMMATIC;

BLOTTING, WESTERN; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; EPOPROSTENOL; GENE EXPRESSION REGULATION; HEMORHEOLOGY; HOMEOSTASIS; HUMANS; ISCHEMIC PRECONDITIONING; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES; STRESS, MECHANICAL; TIME FACTORS; UMBILICAL CORD;

EID: 0034230510     PISSN: 00906964     EISSN: None     Source Type: Journal    
DOI: 10.1114/1.1289472     Document Type: Article

References (29)

1. Achard, F., M. Gilbert, C. Benistant, S. Ben Slama, D. L. DeWitt, W. L. Smith, and M. Lagarde. Eicosapentaenoic and docosahexaenoic acids reduce PGH synthase l expression in bovine aortic endothelial cells. Biochem. Biophys. Res. Commun. 241:513-8, 1997.
2. Chien, S., S. Li, and Y. J. Shyy. Effects of mechanical forces on signal transduction and gene expression in endothelial cells. Hyperstension (Dallas) 31:162-9, 1998.
3. DeWitt, D. L., and E. A. Meade. Serum and glucocorticoid regulation of gene transcription and expression of the prostaglandin H synthase-1 and prostaglandin H synthase-2 isozymes. Arch. Biochem. Biophys. 306:94-102, 1993.
4. Evett, G. E., W. Xie, J. G. Chipman, D. L. Robertson, and D. L. Simmons. Prostaglandin G/H synthase isoenzyme 2 expression in fibroblasts: Regulation by dexamethasone, mitogens, and oncogenes. Arch. Biochem. Biophys. 306:169-77, 1993.
5. Frangos, J. A., S. G. Eskin, L. V. McIntire, and C. L. Ives. Flow effects on prostacyclin production by cultured human endothelial cells. Science 227:1477-9, 1985.
6. Jaffe, E. A., R. L. Nachman, C. G. Becker, and C. R. Minick. Culture of human endothelial cells derived from umbilical veins. J. Clin. Invest. 52:2745-56, 1973.
7. Jun, S. S., Z. Chen, M. C. Pace, and P. W. Shaul. Estrogen upregulates cyclooxygenase-1 gene expression in ovine fetal pulmonary artery endothelium. J. Clin. Invest. 102:176-83, 1998.
8. Kuchan, M. J., and J. A. Frangos. Shear stress regulates endothelin-1 release via protein kinase C and cGMP in cultured endothelial cells. Am. J. Physiol. 264:H150-H156, 1993.
9. Kujubu, D. A., B. S. Fletcher, B. C. Varnum, R. W. Lim, and H. R. Herschman. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J. Biol. Chem. 266:12866-72, 1991.
10. Malek, A., and S. Izumo. Physiological fluid shear stress causes downregulation of endothelin-1 mRNA in bovine aortic endothelium. Am. J. Physiol. 263:C389-96, 1992.
11. Nishida, K., D. G. Harrison, J. P. Navas, A. A. Fisher, S. P. Dockery, M. Uematsu, R. M. Nerem, R. W. Alexander, and T. J. Murphy. Molecular cloning and characterization of the constitutive bovine aortic endothelial cell nitric oxide synthase. J. Clin. Invest. 90:2092-6, 1992.
12. O'Banion, M. K., H. B. Sadowski, V. Winn, and D. A. Young. A serum- and glucocorticoid-regulated 4-kilobase mRNA encodes a cyclooxygenase-related protein. J. Biol. Chem. 266:23261-23267, 1991.
13. Okahara, K., B. Sun, and J.-i. Kambayashi. Upregulation of prostacyclin synthesis-related gene expression by shear stress in vascular endothelial cells. Arterioscler. Thromb. Vasc. Biol. 18:1922-1926 (1998).
14. Pritchard, Jr., K. A., M. K. O'Banion, J. M. Miano, N. Vlasic, U. G. Bhatia, D. A. Young, and M. B. Stemerman. Induction of cyclooxygenase-2 in rat vascular smooth muscle cells in vitro and in vivo. J. Biol. Chem. 269:8504-9, 1994.
15. Ranjan, V., Z. Xiao, and S. L. Diamond. Constitutive NOS expression in cultured endothelial cells is elevated by fluid shear stress. Am. J. Physiol. 38:H550-555, 1995.
16. Rich, G., E. J. Yoder, and S. A. Moore. Regulation of prostaglandin H synthase-2 expression in cerebromicrovascular smooth muscle by serum and epidermal growth factor. J. Cell Physiol. 176:495-505, 1998.
17. Ristimaki, K. Narko, and T. Hla. Down-regulation of cytokine-induced cyclo-oxygenase-2 transcript isoforms by dexamethasone: evidence for post-transcriptional regulation. Br. Biochem. J. 318:325-331, 1996.
18. Ristimaki, A., S. Garfinkel, J. Wessendorf, T. Maciag, and T. Hla. Induction of cyclooxygenase-2 by interleukin-1 alpha. Evidence for post- transcriptional regulation. J. Biol. Chem. 269:11769-75, 1994.
19. Sanduja, S. K., A.-L. Tsai, N. Matijevic-Aleksic, and K. K. Wu. Kinetics of prostacyclin synthesis in PGHs-1-overexpressed endothelial cells. Am. J. Physiol. 267:C1459-1466, 1994.
20. Sharefkin, J. B., S. L. Diamond, S. G. Eskin, and L. V. Mclntire. Fluid flow decreases preproendothelin mRNA levels and suppresses endothelin-1 peptide release in cultured human endothelial cells. J. Vasc. Surg. 14:1-9, 1991.
21. Smeets, E. F., E. J. U. v. Asmuth, C. J. v. d. Linden, J. F. M. Leeuwenberg, and W. A. Buurman. A comparison of substrates for human umbilical vein endothelial cell culture. Biotech. Histochem. 67:241-250, 1992.
22. Smith, W. L., and W. E. Lands. Oxygenation of polyunsaturated fatty acids during prostaglandin biosynthesis by sheep vesicular gland. Biochemistry 11:3276-85, 1972.
23. Tazawa, R., X.-M. Xu, K. K. Wu, and L.-H. Wang. Characterization of the genomic structure, chromosomal location and promoter of human prostaglandin H synthase-2 gene. Biochem. Biophys. Res. Commun. 203:190-199, 1994.
24. Topper, J. N., J. Cai, D. Falb, and M. A. Gimbrone, Jr. Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: cyclooxygenase-2, manganese superoxide dismutase, and endothelial cell nitric oxide synthase are selectively up-regulated by steady laminar shear stress. Proc. Natl. Acad. Sci. U.S.A. 93:10417-22, 1996.
25. Uematsu, M., Y. Ohara, J. P. Navas, K. Nishida, T. J. Murphy, R. W. Alexander, R. M. Nerein, and D. G. Harrison. Regulation of endothelial cell nitric oxide synthase mRNA expression by shear stress. Am. J. Physiol. 269:C1371-8, 1995.
26. Wang, L. H., A. Hajibeigi, X. M. Xu, D. Loose-Mitchell, and K. K. Wu. Characterization of the promoter of human prostaglandin H synthase-1 gene. Biochem. Biophys. Res. Commun. 190:406-11, 1993.
27. Xie, W., and H. R. Herschman. Transcriptional regulation of prostaglandin synthase 2 gene expression by platelet-derived growth factor and serum. J. Biol. Chem. 271:31742-8, 1996.
28. Xu, X.-M., J.-L. Tang, A. Hajibeigi, D. S. Loose-Mitchell, and K. K. Wu. Transcriptional regulation of endothelial constitutive PGHS-1 expression by phorbol ester. Am. J. Physiol. 270:C259-C264, 1996.
29. Yoshizumi, M., H. Kurihara, T. Sugiyama, F. Takaku, M. Yananisawa, T. Masaki, and Y. Yazaki. Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells. Biochem. Biophys. Res. Commun. 161:859-864. 1989.