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We have also attempted a height determination by removing regions of the bilayer applying higher loads and determining step heights at the edges of the removed regions. However, this strategy gave much more ambiguous results. Particularly, as the proximal (surface-bound) monolayer is covalently attached to the surface-anchored polymer it could not be removed completely. However, a higher number of defects was observed. The measured step heights measured on those induced defects varied from 1 to 7.5 nm, i.e., were no larger than those measured from naturally occurring defects in a freshly prepared bilayer structure.
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