Non-invasive measurement of cell membrane associated proton gradients by ion-sensitive field effect transistor arrays for microphysiological and bioelectronical applications

Biosensors and Bioelectronics

Volumn 15, Issue 3-4, 2000, Pages 117-124

  Lehmann, Mirko ^a   Baumann, Werner ^a   Brischwein, Martin ^a   Ehret, Ralf ^a   Kraus, Michael ^a   Schwinde, Anne ^a   Bitzenhofer, Matthias ^a   Freund, Ingo ^a   Wolf, Bernhard ^a  

^a Universitat Rostock Biophysik   (United Kingdom)

Author keywords

Biosensors; Cancer; Cellular sensor; Glycolysis; Microenvironmental pH; pH ISFET

Indexed keywords

ANIMAL CELL CULTURE; BIOCOMPATIBILITY; CELL MEMBRANES; ION SENSITIVE FIELD EFFECT TRANSISTORS; MICROSENSORS; PH SENSORS; PHYSIOLOGY;

GLYCOLYSIS;

BIOSENSORS;

IODOACETIC ACID; TRITON X 100;

ACCURACY; ACIDIFICATION; ARTICLE; BIOSENSOR; CELL CULTURE; CELL MEMBRANE POTENTIAL; CELL PH; COMPUTER ANALYSIS; CONTROLLED STUDY; FLOW INJECTION ANALYSIS; HUMAN; HUMAN CELL; IN VITRO STUDY; INSTRUMENT; TECHNIQUE; TUMOR CELL LINE;

BIOSENSING TECHNIQUES; CELL MEMBRANE; HUMANS; HYDROGEN-ION CONCENTRATION; TUMOR CELLS, CULTURED;

EID: 0034210595     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(00)00065-8     Document Type: Article

References (30)

1. Bard A., Faulkner L. Electrochemical Methods. 1980;Wiley, New York.
2. Baron R. Polarity and membrane transport in osteoclasts. Connect. Tissue Res. 20(1-4):1989;109-120.
3. Baumann, W., 1996. Dissertation. Entwicklung eines Meβsystems zur zelluldren Signalanalyse auf der Basis von Feldeffekttransistoren.
4. Baumann W., Lehmann M., Schwinde A., Ehret R., Brischwein M., Wolf B. Microelectronic sensor systems for microphysiological applications on living cells. Sensors Actuators B. 55:1999;77-89.
5. Daly P.F., Cohen J.S. Magnetic resonance spectroscopy of tumors and potential in vivo clinical applications: a review. Cancer Res. 49:1989;770-779.
6. Dellian M., Helmlinger G., Yuan F., Jain R.K. Fluorescence ratio imaging of interstitial pH in solid tumors: effect of glucose on spatial and temporal gradients. Br. J. Cancer. 74:1996;1206-1215.
7. Ehret R., Baumann W., Brischwein M., Schwinde A., Stegbauer K., Wolf B. Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures. Biosens. Bioelectron. 12(1):1997;29-41.
8. Ehret R., Baumann W., Brischwein M., Schwinde A., Wolf B. On-line control of cellular adhesion with impedance measurements using interdigitated electrode structures. Med. Biol. Eng. Comput. 36:1998;365-370.
9. Flier J.S., Mueckler M.M., Usher P., Lodish H.F. Elevated levels of glucose transport and transporter messenger RNA are induced by ras and src oncogenes. Science. 235:1987;1492-1495.
10. Gerweck L.E., Seetharaman K. Cellular pH gradients in tumor versus normal tissue: potential exploitation for the treatment of cancer. Cancer Res. 56:1996;1194-1198.
11. Gingell D. Cell contact with solid surfaces. Glaser R., Gingell D. Biophysics of the Cell Surface. 1990;263-286 Springer, New York.
12. Griffiths J.R. Are cancer cells acidic? Br. J. Cancer. 64:1991;425-427.
13. Kraus M., Wolf B. Implications of acidic tumor microenvironment for neoplastic growth and cancer treatment, a computer analysis. Tumor Biol. 17:1996;133-154.
14. Kraus M., Wolf B. Physicochemical microenvironment as key regulator for tumor microevolution, invasion, and immune response, targets for endocytotechnological approches in cancer treatment. Endocytobiosis Cell Res. 12:1998;133-156.
15. Kraus M., Severin T., Wolf B. Relevance of microenvironmental pH for self-organized tumor growth and invasion. Anticancer Res. 14:1994;1573-1584.
16. Martin G.R., Jain R.K. Noninvasive measurement of interstitial pH profiles in normal and neoplastic tissue using fluorescence ratio imaging microscopy. Cancer Res. 54:1994;5670-5674.
17. Morita T., Nagaki T., Fukuda I., Okumura K. Clastogenicity of low pH to various cultured mammalian cells. Mutat. Res. 268:1992;297-305.
18. Owicki J., Parce J.W. Biosensors based on the energy metabolism of living cells: the physical chemistry and cell biology of extracellular acidification. Biosens. Bioelectron. 7:1992;225-272.
19. Reynolds T., Rockwell S., Glazer P. Genetic instability induced by the tumor microenvironment. Cancer Res. 56:1996;5754-5757.
20. Severin T., Müller B., Giese G., Uhl B., Wolf B., Hauschildt S., Kreutz W. pH-dependent LAK cell cytotoxicity. Tumor Biol. 15:1994;304-310.
21. Silver I.A., Murrills R.J., Etherington D.J. Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts. Exp. Cell Res. 175:1988;266-276.
22. Stubbs M., Rodrigues L., Howe F.A., Wang J., Jeong K.S., Veech R.L., Griffiths J.R. Metabolic consequences of a reversed pH gradient in rat tumors. Cancer Res. 54:1994;4011-4016.
23. Stubbs M., Veech R.L., Griffiths J.R. Tumor metabolism: the lessons of magnetic resonance spectroscopy. Adv. Enzyme Regul. 35:1995;101-115.
24. Vaupel P.W. The influence of tumor blood flow and microenvironmental factors on the efficacy of radiation, drugs and localized hyperthermia. Klin. Padiatr. 209:1997;243-249.
25. Vaupel P., Kallinowski F., Okunieff P. Blood flow, oxygen and nutrient supply, and metabolic microenvironment of human tumors: a review. Cancer Res. 49:1989;6449-6465.
26. Warburg O. The Metabolism of Tumors. 1930;Arnold Constable, London.
27. Wolf B., Thompson J., von Kleist S. Ultrastructural localisation of carcinoembryonic antigen in a continuous human tumor cell line (LS174T) in relation to morphometric investigations. Anticancer Res. 4:1984;213-220.
28. Wolf B., Brischwein M., Baumann W., Ehret R., Kraus M. Monitoring of cellular signalling and metabolism with modular sensor-technique. The PhysioControl-Microsystem (PCM®). Biosens. Bioelectron. 13:1997;501-509.
29. Wolf B., Kraus M., Sieben U. Potential of microsensor-based feedback bioactuators for biophysical cancer treatment. Biosens. Bioelectron. 12:1997;301-309.
30. Young, PR., Spevacek, SM., 1993. Substratum acidification and proteinase activation by murine B16F10 melanoma cultures. Biochim. Biopys. Acta 1182, 69-74.