The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication

EMBO Journal

Volumn 19, Issue 3, 2000, Pages 421-433

  Schramm, Carolin ^a   Elliott, Sarah ^a   Shevchenko, Anna ^b   Shevchenko, Andrej ^b   Schiebel, Elmar ^a  

^a BEATSON INSTITUTE FOR CANCER RESEARCH   (United Kingdom)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Bbp1p; Kar1p; Mps2p; SPB duplication; Spc29p

Indexed keywords

CELL MEMBRANE PROTEIN;

ALLELE; ARTICLE; CELL CYCLE G1 PHASE; CELL DIVISION; CELL GROWTH; CELL NUCLEUS MEMBRANE; CHROMOSOME SEGREGATION; CONTROLLED STUDY; GENE DOSAGE; GENE ISOLATION; MICROTUBULE; MITOSIS SPINDLE; NONHUMAN; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; YEAST CELL;

SACCHAROMYCETALES;

EID: 0034142341     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.3.421     Document Type: Article

References (45)

1. Adams, I.R. and Kilmartin, J.V. (1999) Localization of core spindle pole body (SPB) components during SPB duplication in Saccharomyces cerevisiae. J. Cell Biol., 145, 809-823.
2. 2+-binding proteins. Proc. Natl Acad. Sci. USA, 83, 5512-5516.
3. Biggins, S. and Rose, M.D. (1994) Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body. J. Cell Biol., 125, 843-852.
4. Biggins, S., Ivanovska, I. and Rose, M.D. (1996) Yeast ubiquitin like genes are involved in duplication of the microtubule organizing center. J. Cell Biol., 133, 1331-1346.
5. Brachat, A., Kilmartin, J.V., Wach, A. and Philippsen, P. (1998) Saccharomyces cerevisiae cells with defective spindle pole body outer plaques accomplish nuclear migration via half-bridge-organized microtubules. Mol. Biol. Cell, 9, 977-991.
6. Bullitt, E., Rout, M.P, Kilmartin, J.V. and Akey, C.W. (1997) The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell, 89, 1077-1086.
7. Byers, B. and Goetsch, L. (1975) Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae. J. Bacteriol., 124, 511-523.
8. Byers, B. and Goetsch, L. (1991) Preparation of yeast cells for thin-section electron microscopy. Methods Enzymol., 194, 602-608.
9. Chial, H.J., Rout, M.P., Giddings, T.H. and Winey, M. (1998) Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies. J. Cell Biol., 143, 1789-1800.
10. Chial, H.J., Giddings, T.H., Siewert, E.A., Hoyt, M.A. and Winey, M. (1999) Altered dosage of the Saccharomyces cerevisiae spindle pole body duplication gene, NDCI, leads to aneuploidy and polyploidy. Proc. Natl Acad. Sci. USA, 96, 10200-10205.
11. Christianson, T.W., Sikorski, R.S., Dante, M., Shero, J.H. and Hieter, P. (1992) Multifunctional yeast high-copy-number shuttle vectors. Gene, 110, 119-122.
12. Danscher, G. (1981) Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry, 71, 81-88.
13. de la Cruz Munoz-Centeno, M., McBratney, S., Monterrosa, A., Byers, B., Mann, C. and Winey, M. (1999) Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Mol. Biol. Cell, 10, 2393-2406.
14. Durfee, T., Becherer, K., Chen, P.L., Yeh, S.H., Yang, Y., Kilburn, A.E., Lee, W.H. and Elledge, S.J. (1993) The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev., 7, 555-569.
15. Elliott, S., Knop, M., Schlenstedt, G. and Schiebel, E. (1999) Spc29p is a component of the Spc110p-subcomplex and is essential for spindle pole body duplication. Proc. Natl Acad. Sci. USA, 96, 6205-6210.
16. Geissler, S., Pereira, G., Spang, A., Knop, M., Souès, S., Kilmartin, J. and Schiebel, E. (1996) The spindle pole body component Spc98p interacts with the γ-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment. EMBO J., 15, 3899-3911.
17. Hutter, K.J. and Eipel, H.E. (1979) Microbial determination by flow cytometry. J. Gen. Microbiol., 113, 369-375.
18. Kilmartin, J.V. and Adams, A.E.M. (1984) Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces cerevisiae. J. Cell Biol., 98, 922-933.
19. Kilmartin, J.V. and Goh, P.Y. (1996) Spc110p: assembly properties and role in the connection of nuclear microtubules to the yeast spindle pole body. EMBO J., 15, 4592-4602.
20. Knop, M. and Schiebel, E. (1997) Spc98p and Spc97p of the yeast γ-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p. EMBO J., 16, 6985-6995.
21. Knop, M. and Schiebel, E. (1998) Receptors determine the cellular localization of a γ-tubulin complex and thereby the site of microtubule formation. EMBO J., 17, 3952-3967.
22. Knop, M., Pereira, G., Geissler, S., Grein, K. and Schiebel, E. (1997) The spindle pole body component Spc97p interacts with the γ-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication. EMBO J., 16, 1550-1564.
23. Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K. and Schiebel, E. (1999) Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast, 15, 963-972.
24. Lauzé, E., Stoelcker, B., Luca, F.C., Weiss, E., Schutz, A.R. and Winey, M. (1995) Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase. EMBO J., 14, 1655-1663.
25. Lupas, A., Van Dyke, M. and Stock, J. (1991) Predicting coiled coils from protein sequences. Science, 252, 1162-1164.
26. Muhlrad, D., Hunter, R. and Parker, R. (1992) A rapid method for localized mutagenesis of yeast genes. Yeast, 8, 79-82.
27. O'Toole, E.T., Winey, M. and McIntosh, J.R. (1999) High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae. Mol. Biol. Cell, 10, 2017-2031.
28. Pereira, G., Knop, M. and Schiebel, E. (1998) Spc98p directs the yeast γ-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body. Mol. Biol. Cell, 9, 775-793.
29. Pereira, G., Grueneberg, U., Knop, M. and Schiebel, E. (1999) Interaction of the yeast γ-tubulin complex binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy. EMBO J., 18, 4180-4196.
30. Rose, M.D. and Fink, G.R. (1987) KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast. Cell, 48, 1047-1060.
31. Rout, M.P. and Kilmartin, J.V. (1990) Components of the yeast spindle and spindle pole body. J. Cell Biol., 111, 1913-1927.
32. Shevchenko, A., Jensen, O.N., Podtelejnikov, A.V., Sagliocco, F., Wilm, M., Vorm, O., Mortensen, P., Boucherie, H. and Mann, M. (1996) Linking genome and proteome by mass spectrometry: large scale identification of yeast proteins from two dimensional gels. Proc. Natl Acad. Sci. USA, 93, 14440-14445.
33. Sikorski, R.S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.
34. Spang, A., Courtney, I., Fackler, U., Matzner, M. and Schiebel, E. (1993) The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body. J. Cell Biol., 123, 405-416.
35. Spang, A., Courtney, I., Grein, K., Matzner, M. and Schiebel, E. (1995) The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body. J. Cell Biol., 128, 863-877.
36. Spang, A., Geissler, S., Grein, K. and Schiebel, E. (1996) γ-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J. Cell Biol., 134, 429-441.
37. Studier, F.W. and Moffat, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes. J. Mol. Biol., 189, 113-130.
38. Sundberg, H.A. and Davis, T.A. (1997) A mutational analysis identifies three functional regions of the spindle pole body component Spc110p in Saccharomyces cerevisiae. Mol. Biol. Cell, 8, 2575-2590.
39. Vallen, E.A., Hiller, M.A., Scherson, T.Y. and Rose, M.D. (1992) Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol., 117, 1277-1287.
40. Vallen, E.A., Ho, W., Winey, M. and Rose, M.D. (1994) Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. Genetics, 137, 407-422.
41. Wach, A., Brachat, A., Alberti-Segui, C., Rebischung, C. and Philippsen, P. (1997) Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast, 13, 1065-1075.
42. Weiss, E. and Winey, M. (1996) The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. J. Cell Biol., 132, 111-123.
43. Wigge, P.A., Jensen, O.N., Holmes, S., Souès, S., Mann, M. and Kilmartin, J.V. (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol., 141, 967-977.
44. Winey, M., Goetsch, L., Baum, P. and Byers, B. (1991) MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication. J. Cell Biol., 114, 745-754.
45. Winey, M., Hoyt, M.A., Chan, C., Goetsch, L., Botstein, D. and Byers, B. (1993) NDC1: a nuclear periphery component required for yeast spindle pole body duplication. J. Cell Biol., 122, 743-751.