A mutational analysis identifies three functional regions of the spindle pole component Spc110p in Saccharomyces cerevisiae

Molecular Biology of the Cell

Volumn 8, Issue 12, 1997, Pages 2575-2590

  Sundberg, Holly A ^a   Davis, Trisha N ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; DNA SYNTHESIS INHIBITION; GENE DUPLICATION; GENE MUTATION; GENETIC COMPLEMENTATION; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; SACCHAROMYCES CEREVISIAE; SPINDLE CELL; TEMPERATURE SENSITIVE MUTANT;

SACCHAROMYCES CEREVISIAE;

EID: 0030660673     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.12.2575     Document Type: Article

References (33)

1. Berger, B., Wilson, D.B., Wolf, E., Tonchev, T., Milla, M., and Kim, P.S. (1995). Predicting coiled coils by use of pairwise residue correlations. Proc. Natl. Acad. Sci. USA 92, 8259-8263.
2. Boeke, J.D., Trueheart, J., Natsoulis, G., and Fink, G.R. (1987). 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154, 164-175.
3. Bullitt, E., Rout, M.P., Kilmartin, J.V., and Akey, C.W. (1997). The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell 89, 1077-1086.
4. Byers, B. (1981). Cytology of the yeast life cycle. In: The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance, ed. J.N. Strathern, E.W. Jones, and J.R. Broach, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 59-96.
5. Dasgupta, M., and Blumenthal, D.K. (1995). Characterization of the regulatory domain of the gamma-subunit of phosphorylase kinase. The two noncontiguous calmodulin-binding subdomains are also autoinhibitory. J. Biol. Chem. 270, 22283-22289.
6. Davis, T. (1990). Genetic analysis of calcium-binding proteins in yeast. In: Stimulus Response Coupling: The Role of Intracellular Calcium-binding Proteins, ed. V.L. Smith and J.R. Dedman, Boston: CRC Press, 237-249.
7. Davis, T.N. (1992). A temperature-sensitive calmodulin mutant loses viability during mitosis. J. Cell Biol. 118, 607-617.
8. Fincham, J.R.S. (1966). Genetic Complementation. New York: W.A. Benjamin, Inc.
9. Friedman, D.B., Sundberg, H.A., Huang, E., and Davis, T.N. (1996). The 110-kD spindle pole body component of Saccharomyces cercvisiae is a phosphoprotein that is modified in a cell cycle-dependent manner. J. Cell Biol. 132, 903-914.
10. Geiser, J.R., Sundberg, H.A., Chang, B.H., Muller, E.G.D., and Davis, T.N. (1993). The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. Mol. Cell. Biol. 23, 7913-7924.
11. Geiser, J.R., van-Tuinen, D., Brockerhoff, S.E., Neff, M.M., and Davis, T.N. (1991). Can calmodulin function without binding calcium? Cell 65, 949-959.
12. Hardwick, K.G., and Murray, A.W. (1995). Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast. J. Cell Biol. 232, 709-720.
13. Harris, W.R., Malencik, D.A., Johnson, C.M., Carr, S.A., Roberts, G.D., Byles, C.A., anderson, S.R., Heilmeyer, L.M., Jr., Fischer, E.H., and Crabb, J.W. (1990). Purification and characterization of catalytic fragments of phosphorylase kinase gamma subunit missing a calmodulin-binding domain. J. Biol. Chem. 265, 11740-11745.
14. Kilmartin, J.V., Dyos, S.L., Kershaw, D., and Finch, J.T. (1993). A spacer protein in the Saccharomyces cerevisiae spindle pole body whose transcript is cell cycle-regulated. J. Cell Biol. 223, 1175-1184.
15. Kilmartin, J.V., and Goh, P.-Y. (1996). Spc110p: assembly properties and role in the connection of nuclear microtubules to the yeast spindle pole body. EMBO J. 25, 4592-4602.
16. Knop, M., Pereira, G., Geissler, S., Grein, K., and Schiebel, E. (1997). The spindle pole body component Spc97p interacts with the γ-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication. EMBO J. 16, 1550-1564.
17. Kunkel, T.A., Roberts, J.D., and Zakour, R.A. (1987). Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 254, 367-382.
18. Li, R., and Murray, A.W. (1991). Feedback control of mitosis in budding yeast. Cell 66, 519-531.
19. Marschall, L.G., Jeng, R.L., Mulholland, J., and Stearns, T. (1996). Analysis of Tub4p, a yeast γ-tubulin-like protein: implications for microtubule-organizing center function. J. Cell Biol. 234, 443-454.
20. Mirzayan, C., Copeland, C.S., and Snyder, M. (1992). The NUF1 gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeleton. J. Cell Biol. 116, 1319-1332.
21. Muhlrad, D., Hunter, R., and Parker, R. (1992). A rapid method for localized mutagenesis of yeast genes. Yeast 8, 79-82.
22. 1 interval of the cell cycle. J. Biol. Chem. 266, 9194-9202.
23. Rout, M.P., and Kilmartin, J.V. (1990). Components of the yeast spindle and spindle pole body. J. Cell Biol. 111, 1913-1927.
24. Sikorski, R.S., and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
25. Sobel, S.G., and Snyder, M. (1995). A highly divergent γ-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae. J. Cell Biol. 131, 1775-1788.
26. Spang, A., Geissler, S., Grein, K., and Schiebel, E. (1996a). γ-Tubulinlike Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J. Cell Biol. 134, 429-441.
27. Spang, A., Grein, K., and Schiebel, E. (1996b). The spacer protein Spell 110p targets calmodulin to the central plaque of the yeast spindle pole body. J. Cell Sci. 109, 2229-2237.
28. Stirling, D.A., Rayner, T.F., Prescott, A.R., and Stark, M.J.R. (1996). Mutations which block the binding of calmodulin to Spell 110p cause multiple mitotic defects. J. Cell Sci. 209, 1297-1310.
29. Stirling, D.A., Welch, K.A., and Stark, M.J.R. (1994). Interaction with calmodulin is required for the function of Spc110p, an essential component of the yeast spindle pole body. EMBO J. 13, 4329-4342.
30. Sundberg, H.A., Goetsch, L., Byers, B., and Davis, T.N. (1996). Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body components. J. Cell Biol. 133, 111-124.
31. Willingham, M.C., Wehland, J., Klee, C.B., Richert, N.D., Rutherford, A.V., and Pastan, I.H. (1983). Ultra structural immunocyto-chemical localization of calmodulin in cultured cells. J. Histochem. Cytochem. 32, 445-461.
32. Woolfson, D.N., and Alber, T. (1995). Predicting oligomerization states of coiled coils. Protein Sci. 4, 1596-1607.
33. Zhu, G., Muller, E.G.D., Amacher, S.L., Northrop, J.L., and Davis, T.N. (1993). A dosage-dependent suppressor of a temperature-sensitive calmodulin mutant encodes a protein related to the fork head family of DNA-binding proteins. Mol. Cell. Biol. 23, 1779-1787.