Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies

Journal of Cell Biology

Volumn 143, Issue 7, 1998, Pages 1789-1800

  Chial, Heidi J ^a   Rout, Michael P ^b   Giddings Jr , Thomas H ^a   Winey, Mark ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Ndc1p; Nuclear pore complex; Pom152p; Spindle pole body; Yeast

Indexed keywords

MEMBRANE PROTEIN;

ARTICLE; CELL NUCLEUS; GENE; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; MITOSIS SPINDLE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE;

CENTROSOME; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FUNGAL PROTEINS; GENE DELETION; MACROMOLECULAR SUBSTANCES; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MICROSCOPY, IMMUNOELECTRON; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0032576588     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.143.7.1789     Document Type: Article

References (17)

1. Aitchison, J.D., G. Blobel, and M.P. Rout. 1995a. Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport. J. Cell Biol. 131:1659-1675.
2. Aitchison, J.D., M.P. Rout, M. Marelli, G. Blobel, and R.W. Wozniak. 1995b. Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J. Cell Biol. 131:1133-1148.
3. Ausubel, J.D., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1994. Current Protocols in Molecular Biology. John Wiley and Sons, New York.
4. Baudin, A., O. Ozier-Kalogeropoulos, A. Denouel, F. Lacroute, and C. Cullin. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21:3329-3330.
5. Bucci, M., and S.R. Wente. 1997. In vivo dynamics of nuclear pore complexes in yeast. J. Cell Biol. 136:1185-1199.
6. Bucci, M., and S.R. Wente. 1998. A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly. Mol. Biol. Cell. 9:2439-2461.
7. Bullitt, E., M.P. Rout, J.V. Kilmartin, and C.W. Akey. 1997. The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell. 89:1077-1086.
8. Cormack, B.P., G. Bertram, M. Egerton, N.A. Gow, S. Falkow, and A.J. Brown. 1997. Yeast-enhanced green fluorescent protein (yEGFP) a reporter of gene expression in Candida albicans. Microbiology. 143:303-311.
9. Davis, L.I., and G. Blobel. 1986. Identification and characterization of a nuclear pore complex protein. Cell. 45:699-709.
10. Davis, L.I., and G. Blobel. 1987. Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway. Proc. Nail. Acad. Sci. USA. 84:7552-7556.
11. Davis, L.I., and G.R. Fink. 1990. The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell. 61:965-978.
12. Ding, R., R.R. West, D.M. Morphew, B.R. Oakley, and J.R. McIntosh. 1997. The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds. Mol. Biol. Cell. 8:1461-1479.
13. Donaldson, A.D., and J.V. Kilmartin. 1996. Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPB) with an essential function during SPB duplication. J. Cell Biol. 132:887-901.
14. Doye, V., and E. Hurt. 1997. From nucleoporins to nuclear pore complexes. Curr. Opin. Cell Biol. 9:401-411.
15. Doye, V., R. Wepf, and E.C. Hurt. 1994. A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO (Eur. Mol. Biol. Organ.) J. 13:6062-6075.
16. Elledge, S.J., and R.W. Davis. 1988. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene. 70:303-312.
17. Fernandez, J., L. Andrews, and S.M. Mische. 1994. An improved procedure for enzymatic digestion of polyvinyldiene difluoride-bound proteins for internal sequence analysis. Anal. Biochem. 218:112-117.