Helicobacter pylori possesses two CheY response regulators and a histidine kinase sensor, CheA, which are essential for chemotaxis and colonization of the gastric mucosa

Infection and Immunity

Volumn 68, Issue 4, 2000, Pages 2016-2023

  Foynes, Susan ^a,d   Dorrell, Nick ^a   Ward, Stephen J ^a,d   Stabler, Richard A ^a   McColm, Andy A ^b   Rycroft, Andrew N ^c   Wren, Brendan W ^a  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c ROYAL VETERINARY COLLEGE   (United Kingdom)

^d IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN HISTIDINE KINASE;

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL VIRULENCE; CHEMOTAXIS; FLAGELLUM; GENE MUTATION; HELICOBACTER PYLORI; IMMUNE RESPONSE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; STOMACH MUCOSA; SWINE;

ANIMALS; BACTERIAL PROTEINS; BLOOD; CHEMOTAXIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; FLAGELLA; GASTRIC MUCOSA; HELICOBACTER PYLORI; MEMBRANE PROTEINS; MICE; MOVEMENT; MUTAGENESIS, SITE-DIRECTED; SWINE; VIRULENCE;

EID: 0034061330     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.4.2016-2023.2000     Document Type: Article

References (41)

1. Adler, J. 1973. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J. Gen. Microbiol. 74:77-91.
2. Allan, E. 1997. Identification and characterisation of potential pathogenicity determinants of Helicobacter pylori. Ph.D. thesis. University of London, London, United Kingdom.
3. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
4. Beier, D., G. Spohn, R. Rappuoli, and V. Scarlato. 1997. Identification and characterization of an operon of Helicobacter pylori that is involved in motility and stress adaptation. J. Bacteriol. 179:4676-4683.
5. Blaser, M. J. 1997. Ecology of Helicobacter pylori in the human stomach. J. Clin. Investig. 100:759-762.
6. Danon, S. J., and K. A. Eaton. 1998. Isogenic flagella mutants of Helicobacter pylori: candidates for an attenuated vaccine? Gut 43(Suppl. 2):A38.
7. Dorrell, N., V. G. Gyselman, S. Foynes, S. R. Li, and B. W. Wren. 1996. Improved efficiency of inverse PCR mutagenesis (IPCRM). BioTechniques 21:604-608.
8. Douce, G., M. Fontana, M. Pizza, R. Rappuoli, and G. Dougan. 1997. Intranasal immunogenicity and adjuvanticity of site-directed mutant derivatives of cholera toxin. Infect. Immun. 65:2821-2828.
9. Eaton, K. A., S. Suerbaum, C. Josenhans, and S. Krakowka. 1996. Colonization of gnotobiotic piglets by Heliobacter pylori deficient in two flagellin genes. Infect. Immun. 64:2445-2448.
10. Eisenbach, M. 1996. Control of bacterial chemotaxis. Mol. Microbiol. 20: 903-910.
11. Ferrero, R. L., V. Cussac, P. Courcoux, and A. Labigne. 1992. Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange. J. Bacteriol. 174:4212-4217.
12. Foynes, S., N. Dorrell, S. J. Ward, Z. W. Zhang, A. A. McColm, M. J. G. Farthing, and B. W. Wren. 1999. Functional analysis of the roles of FliQ and FlhB in flagellar expression in Helicobacter pylori. FEMS Microbiol. Lett. 174:33-39.
13. D-dependent gene encodes a novel protein with both CheW and CheY homologous domains. J. Bacteriol. 176:2727-2735.
14. Haas, R., T. F. Meyer, and J. P. M. Vanputten. 1993. Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis. Mol. Microbiol. 8:753-760.
15. Hazell, S. L., A. Lee, L. Brady, and W. Hennessy. 1986. Campylobacter pyloridis and gastritis: association with intercellular spaces and adaptation to an environment of mucus as important factors in colonization of the gastric epithelium. J. Infect. Dis. 153:658-663.
16. Hess, J. F., R. B. Bourret, and M. I. Simon. 1988. Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis. Nature 336:139-143.
17. Jenks, P. J., S. Foynes, S. J. Ward, C. Constantinidou, C. W. Penn, and B. W. Wren. 1997. A flagellar-specific ATPase (FliI) is necessary for flagellar export in Helicobacter pylori. FEMS Microbiol. Lett. 152:205-211.
18. Karim, Q. N., R. P. H. Logan, J. Puels, A. Karnholz, and M. L. Worku. 1998. Measurement of motility of Helicobacter pylori, Campylobacter jejuni and Escherichia coli by real time computer tracking using the Hobson Bac-Tracker. J. Clin. Pathol. 51:623-628.
19. Kirschner, D. E., and M. J. Blaser. 1995. The dynamics of Helicobacter pylori infection of the human stomach. J. Theor. Biol. 176:281-290.
20. Krakowka, S., D. R. Morgan, W. G. Kraft, and R. D. Leunk. 1987. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect. Immun. 55:2789-2796.
21. Kuipers, E. J. 1997. Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer. Aliment. Pharmacol. Ther. 11:71-88.
22. Lee, A., J. L. O'Rourke, P. J. Barrington, and T. J. Trust. 1986. Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse cecal model. Infect. Immun. 51:536-546.
23. Lee, A., J. O'Rourke, M. C. DeUngria, B. Robertson, G. Daskalopoulos, and M. F. Dixon. 1997. A standardized mouse model of Helicobacter pylori infection: introducing the Sydney strain. Gastroenterology 112:1386-1397.
24. Maddock, J. R., and L. Shapiro. 1993. Polar location of the chemoreceptor complex in the Escherichia coli cell. Science 259:1717-1723.
25. McColm, A. A. 1997. Nonprimate animal models of H. pylori infection, p. 235-251. In C. L. Clayton and H. L. T. Mobley (ed.), Helicobacter pylori protocols. Humana Press, Totowa, N.J.
26. Mizote, T., H. Yoshiyama, and T. Nakazawa. 1997. Urease-independent chemotactic responses of Helicobacter pylori to urea, urease inhibitors, and sodium bicarbonate. Infect. Immun. 65:1519-1521.
27. Moulton, R. C., and T. C. Montie. 1979. Chemotaxis by Pseudomonas aeruginosa. J. Bacteriol. 137:274-280.
28. Nakamura, H., H. Yoshiyama, H. Takeuchi, T. Mizote, K. Okita, and T. Nakazawa. 1998. Urease plays an important role in the chemotactic motility of Helicobacter pylori in a viscous environment. Infect. Immun. 66:4832-4837.
29. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
30. Sanders, D. A., B. L. Gillececastro, A. M. Stock, A. L. Burlingame, and D. E. Koshland. 1989. Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY. J. Biol. Chem. 264:21770-21778.
31. Segal, E. D., and L. S. Tompkins. 1993. Transformation of Helicobacter pylori by electroporation. BioTechniques 14:225-226.
32. Segal, E. D., S. Falkow, and L. S. Tompkins. 1996. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc. Natl. Acad. Sci. USA 93:1259-1264.
33. Stock, J. B., and M. G. Surette. 1996. Chemotaxis, p. 1103-1129. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
34. Suerbaum, S. 1995. The complex flagella of gastric Helicobacter species. Trends Microbiol. 3:168-170.
35. Tomb, J. F., O. White, A. R. Kerlavage, R. A. Clayton, G. G. Sutton, R. D. Fleischmann, K. A. Ketchum, H. P. Klenk, S. Gill, B. A. Dougherty, K. Nelson, J. Quackenbush, L. X. Zhou, E. F. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H. G. Khalak, A. Glodek, K. McKenney, L. M. Fitzgerald, N. Lee, M. D. Adams, E. K. Hickey, D. E. Berg, J. D. Gocayne, T. R. Utterback, J. D. Peterson, J. M. Kelley, M. D. Cotton, J. M. Weidman, C. Fujii, C. Bowman, L. Watthey, E. Wallin, W. S. Hayes, M. Borodovsky, P. D. Karp, H. O. Smith, C. M. Fraser, and J. C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539-547.
36. Trieu-Cuot, P., G. Gerbaud, T. Lambert, and P. Courvalin. 1985. In vivo transfer of genetic information between gram-positive and gram-negative bacteria. EMBO J. 4:3583-3587.
37. Turner, G. A., R. P. H. Logan, R. Chinnery, A. Cockayne, C. J. Hawkey, and S. P. Borriello. 1997. Trefoil peptides are unique chemotaxins for Helicobacter pylori. Gastroenterology 112:A1107.
38. Volz, K. 1993. Structural conservation in the CheY superfamily. Biochemistry 32:11741-11753.
39. Wren, B. W., S. M. Colby, R. R. Cubberley, and M. J. Pallen. 1992. Degenerate PCR primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria. FEMS Microbiol. Lett. 99:287-291.
40. Wren, B. W., J. Henderson, and J. M. Ketley. 1994. A PCR-based strategy for the rapid construction of defined bacterial deletion mutants. BioTechniques 16:994-996.
41. Yao, R. J., D. H. Burr, and P. Guerry. 1997. CheY-mediated modulation of Campylobacter jejuni virulence. Mol. Microbiol. 23:1021-1031.