Expression of CYP1A1, CYP1B1 and CYP3A, and polycyclic aromatic hydrocarbon-DNA adduct formation in bronchoalveolar macrophages of smokers and non-smokers

International Journal of Cancer

Volumn 86, Issue 5, 2000, Pages 610-616

  Piipari, Ritva ^a   Savela, Kirsti ^a   Nurminen, Tuula ^a   Hukkanen, Janne ^b   Raunio, Hannu ^b   Hakkola, Jukka ^b   Mäntylä, Timo ^c   Beaune, Philippe ^d   Edwards, Robert J ^e   Boobis, Alan R ^e   Anttila, Sisko ^a  

^a FINNISH INSTITUTE OF OCCUPATIONAL HEALTH   (Finland)

^b UNIVERSITY OF OULU   (Finland)

^c Laakso Hospital   (Finland)

^d INSERM   (France)

^e HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC HYDROCARBON; CIGARETTE SMOKE; CYTOCHROME P450 1B1; CYTOCHROME P450 3A; CYTOCHROME P450 ISOENZYME; DNA; ETHOXYRESORUFIN DEETHYLASE; NUCLEASE; PHOSPHORUS 32; UNCLASSIFIED DRUG;

ADULT; AGE; AGED; ARTICLE; CANCER RISK; CLINICAL ARTICLE; DNA ADDUCT; DRUG METABOLISM; FEMALE; GENDER; HUMAN; IMMUNOBLOTTING; LUNG ALVEOLUS EPITHELIUM; LUNG ALVEOLUS MACROPHAGE; LUNG CARCINOGENESIS; MALE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SMOKING; SMOKING HABIT;

ADULT; AGED; AGED, 80 AND OVER; ARYL HYDROCARBON HYDROXYLASES; BRONCHI; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP3A; CYTOCHROME P-450 ENZYME SYSTEM; DNA ADDUCTS; HUMANS; MACROPHAGES, ALVEOLAR; MIDDLE AGED; OXIDOREDUCTASES, N-DEMETHYLATING; POLYCYCLIC HYDROCARBONS, AROMATIC; SMOKING;

EID: 0034059311     PISSN: 00207136     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0215(20000601)86:5<610::AID-IJC2>3.0.CO;2-M     Document Type: Article

References (21)

1. ANTTILA, S., HIETANEN, E., VAINIO, H., CAMUS, A.-M., GELBOIN, H.V., PARK, S.S., HEIKKILÄ, L., KARJALAINEN, A. and BARTSCH, H., Smoking and peripheral type of cancer are related to high levels of pulmonary cytochrome P4501A in lung cancer patients. Int. J. Cancer, 47, 681-685 (1991).
2. ANTTILA, S., HUKKANEN, J., HAKKOLA, J., STJERNVALL, T., BEAUNE, P., EDWARDS, R.J., BOOBIS, A.R., PELKONEN, O. and RAUNIO, H., Expression and localisation of CYP3A4 and CYP3A5 in human lung. Amer. J. Respir. Cell Mol. Biol., 16, 242-249 (1997).
3. BELLOC, C., BAIRD, S., COSME, J., LECOEUR, S., GAUTIER, J.-C., CHALLINE, D., DE WAZIERS, L., FLINOIS, J.-P. and BEAUNE, P.H., Human cytochromes P450 expressed in Escherichia coli: production of specific antibodies. Toxicology, 106, 207-219 (1996).
4. EDWARDS, R.J., ADAMS, D.A., WATTS, P.S., DAVIES, D.S. and BOOBIS, A.R., Development of a comprehensive panel of antibodies against the major xenobiotic metabolising forms of cytochrome P450 in human. Biochem. Pharmacol., 56, 377-387 (1998).
5. 32P-postlabelling and aryl hydrocarbon hydroxylase activity in lung parenchyma of smokers and ex-smokers. Carcinogenesis, 12, 1301-1305 (1991).
6. HAKKOLA, J., PASANEN, M., PELKONEN, O., HUKKANEN, J., EVISALMI, S., ANTTILA, S., RANE, A., MÄNTYLÄ, M., PURKUNEN, R., SAARIKOSKI, S., TOOMIN, M. and RAUNIO, H., Expression of CYP1B1 in human adult and fetal tissues and differential inducibility of CYP1B1 and CYP1A1 by Ah receptor ligands. Carcinogenesis, 18, 391-397 (1997).
7. HARRIS, C.C., Hsu, I.C. and STONER, G.D., Human pulmonary alveolar macrophages metabolise benzo(a)pyrene to proximate and ultimate mutagens. Nature, 272, 633-634 (1978).
8. HUKKANEN, J., HAKKOLA, J., ANTTILA, S., PIIPARI, R., KARJALAINEN, A., PELKONEN, O. and RAUNIO, H., Detection of mRNA encoding xenobiotic-metabolizing cytochrome P450s in human bronchoalveolar macrophages and peripheral blood lymphocytes. Mol. Carcinogenesis, 20, 224-230 (1997).
9. IZZOTTI, A., ROSSI, G.A., BAGNASCO, M. and DE FLORA, S., Benzo(a)pyrene diolepoxide-DNA adducts in alveolar macrophages of smokers. Carcinogenesis, 12, 1281-1285 (1991).
10. KIM, J.H., STANSBURY, K.H., WALKER, N.J., TRUSH, M.A., STRICKLAND, P.T. and SUTTER, T.R., Metabolism of benzo[a]pyrene and benzo[a]pyrene-7,8,-diol by human cytochrome P450 1B1. Carcinogenesis, 19, 1847-1853 (1998).
11. LINDER, J. and RENNARD, S.I., Bronchoalveolar lavage, pp. 21-22, 27-29, ASCP Press, Chicago (1988).
12. MCLEMORE, T.L., ADELBERG, S., LIU, M.C., MCMAHON, N.A., YU, S.J., HUBBARD, W.C., CZERWINSKI, M., WOOD, T.G., STORENG, R., LUBET, R.A., EGGLESTON, J.C., BOYD, M.R. and HINES, R.N., Expression of CYP1A1 gene in patients with lung cancer: evidence for cigarette smoke-induced gene expression in normal lung tissue and for altered gene regulation in primary pulmonary carcinomas. J. natl. Cancer Inst., 82, 1333-1339 (1990).
13. PHILLIPS, D.H., HEWER, A., MARTIN, C.N., GARNER, R.C. and KING, M.M., Correlation of DNA adduct levels in human lung with cigarette smoking. Nature (Lond.), 336, 790-792 (1988).
14. PHILLIPS, D.H., SCHOKET, B., HEWER, A., BAILEY, E., KOSTIC, S. and VINCZE, L., Influence of cigarette smoking on the levels of DNA adducts in human bronchial epithelium and white blood cells. Int. J. Cancer, 46, 569-575 (1990).
15. 32P-postlabeling lest for structurally diverse DNA adducts. Carcinogenesis, 7, 1543-1551 (1986).
16. ROBERTS-THOMSON, S.J., MCMANUS, M.E., TUKEY, R.H., GONZALEZ, F.J. and HOLDER, G.M., The catalytic activity of four expressed human cytochrome P450s towards benzo(a)pyrene and the isomers of its proximate carcinogen. Biochem. biophys. Res. Comm., 192, 1373-1379 (1993).
17. SAS Institute, Inc., SAS/STAT user's guide, version 6 (4th ed.), vols. 1 and 2, SAS Institute, Inc., Gary, NC (1989).
18. 32P-postlabelling assay. Carcinogenesis, 12, 503-508 (1991).
19. SHIMADA, T., YAMAZAKI, H., MIMURA, M., WAKAMIYA, N., UENG, Y.-F., GUENGERICH, F.P. and INUI, Y., Characterization of microsomal cytochrome P450 enzymes involved in the oxidation of xenobiotic chemicals in human fetal livers and adult lungs. Drug Metab. Dispos., 24, 515-522 (1996).
20. WHEELER, C.W., WRIGHTON, S.A. and GUENTHNER, T.M., Detection of human lung cytochromes P450 that are immunochemically related to cytochrome P450IIE1 and cytochrome P450 IIIA. Biochem. Pharmacol., 44, 183-186 (1992).
21. WILLEY, J.C., COY, E.L., FRAMPTON, M.W., TORRES, A., APOSTOLAKOS, M.J., HOEHN, G., SCHUERMANN, W.H., THILLY, W.G., OLSON, D.E., HAMMERSLEY, J.R., CRESPI, C.L. and UTELL, M.J., Quantitative RT-PCR measurement of cytochromes P450 1A1, 1B1, and 2B7, microsomal epoxide hydrolase, and NADPH oxidoreductase expression in lung cells of smokers and nonsmokers. Amer. J. Respir. Cell Mol. Biol., 17, 114-124 (1997).