Quantitative RT-PCR Measurement of Cytochromes p450 1A1, 1B1, and 2B7, Microsomal Epoxide Hydrolase, and NADPH Oxidoreductase Expression in Lung Cells of Smokers and Nonsmokers

American Journal of Respiratory Cell and Molecular Biology

Volumn 17, Issue 1, 1997, Pages 114-124

  Willey, James C ^a   Coy, Erin L ^b   Frampton, Mark W ^b   Torres, Alfonso ^b   Apostolakos, Michael J ^b   Hoehn, Gerard ^b   Schuermann, Wolfgang H ^b   Thilly, William G ^b   Olson, Dan E ^b   Hammersley, Jeffrey R ^b   Crespi, Charles L ^b   Utell, Mark J ^b  

^a MEDICAL COLLEGE OF OHIO   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CYP2B7P1 PROTEIN, HUMAN; CYTOCHROME P 450 CYP1B1; CYTOCHROME P-450 CYP1B1; CYTOCHROME P450; CYTOCHROME P450 1A1; EPOXIDE HYDROLASE; PRIMER DNA; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; UNSPECIFIC MONOOXYGENASE;

ADULT; ARTICLE; BIOSYNTHESIS; BRONCHOSCOPY; COMPARATIVE STUDY; ENZYMOLOGY; FEMALE; HUMAN; LUNG; MALE; METABOLISM; METHODOLOGY; MICROSOME; POLYMERASE CHAIN REACTION; REFERENCE VALUE; SMOKING;

ADULT; ARYL HYDROCARBON HYDROXYLASES; BRONCHOSCOPY; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; EPOXIDE HYDROLASES; FEMALE; HUMANS; LUNG; MALE; MICROSOMES; NADPH OXIDASE; POLYMERASE CHAIN REACTION; REFERENCE VALUES; SMOKING;

EID: 0031178547     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.17.1.2783     Document Type: Article

References (43)

1. Harris, C. C. 1991. Chemical and physical carcinogenesis: advances and perspectives for the 1990s. Cancer Res. 51:5023S-5044S.
2. Samel, J. M., and M. J. Utell. 1991. The environment and the lung: changing perspectives. JAMA 266:670-675.
3. Dockery, D. W., A. C. Pope, III, X. Xu, J. D. Spengler, J. H. Ware, M. E. Fay, B. G. Ferris, Jr., and F. E. Speizer. 1993. An association between air pollution and mortality in six U.S. cities. N. Engl. J. Med. 329:1753-1759.
4. Vena, J. E. 1982. Air pollution as a risk factor in lung cancer. Am. J. Epidemiol. 116:42-56.
5. Guengerich, F. P. 1994. Catalytic selectivity of human cytochrome P450 enzymes: relevance to drug metabolism and toxicity. Toxicol. Lett. 70:133-138.
6. Shimada, T., M. V. Martin, D. Pruess-Schwartz, L. J. Marnett, and F. P. Guengerieh. 1989. Roles of individual human cytochrome P-450 enzymes in the bioactivation of benzo(a)pyrene, 7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene, and other dihydrodiol derivatives of polycyclic aromatic hydrocarbons. Cancer Res. 49:6304-6312.
7. Guengerieh, F. P., T. Shimada, K. D. Raney, C.-H. Yun, D. J. Meyer, B. Ketterer, T. M. Harris, J. D. Groopman, and F. F. Kadlubar. 1992. Elucidation of catalytic specificities of human cytochrome P450 and glutathione S-transferase enzymes and relevance to molecular biology. Env. Health Persp. 98:75-80.
8. Shimada, T., C.-H. Yun, H. Yamazaki, J.-C. Gautier, P. H. Beaune, and F. P. Guengerich, 1992. Characterization of human lung microsomal cytochrome P-450 1A1 and its role in oxidation of chemical carcinogens. Mol. Pharm. 41:856-864.
9. Thakker, D. R., A. Y. Yagi, A. Y. H. Lu, W. Levin, A. H. Conney, and D.M. Jerina. 1976. Metabolism of benzo[a]pyrene: conversion of (±)-trans-7,8-dihydroxy-7,8-dihydrohenzo[a]pyrene to highly mutagenic 7,8-diol-9,10-epoxides. Proc. Natl. Acad. Sci. USA 73:3381-3385.
10. Hecht, S. S., and D. Hoffmann. 1988. Advances in tobacco carcinogenesis. In Chemical Carcinogenesis and Mutagenesis I. C. S. Cooper and P. L. Grover, editors. Springer-Verlag, New York. 63-102.
11. McWilliams, J. E., B. J. Sanderson, E. L. Harris, K. E. Richert-Boe, and W. D. Henner. 1995. Glutathione-s-transferase M1 (GST M1) deficiency and lung cancer risk. Cancer Epidemiol. Biomarkers Preven. 4:589-594.
12. Nakachi, K., K. Imai, S. Hagashi, and K. Kawjiri. 1993. Polymorphisms of the CYP1A1 and glutathione-s-transferase genes associated with susceptibility to lung cancer in relation to cigarette dose in a Japanese population. Cancer Res. 53:2994-2999.
13. Anttila, S., H. Vainio, E. Hietanen, A.-M. Camus, C. Malaveille, G. Brun, K. Husgafvel-Pursianen, L. Heikkila, A. Karjalainen, and H. Bartsch. 1992. Immunohistochemical detection of pulmonary cytochrome P4501A and metabolic activities associated with P4501A1 and P4501A2 isozymes in lung cancer patients. Environ. Health Perspect. 98:179-182.
14. Hayashi, S.-I., J. Watanabe, K. Nakachi, H. Eguchi, O. Gotoh, and K. Kawajiri. 1994. Interindividual difference in expression of human Ah receptor and related P450 genes. Carcinogenesis 15:801-806.
15. Autrup, H., F. C. Wefald, A. M. Jeffrey, H. Tate, R. D. Schwartz, B. F. Trump, and C. C. Harris. 1980. Metabolism of benzo[a]pyrene by cultured tracheobronchial epithelial tissues from mice, rats, hamsters, bovines and humans. Int. J. Cancer 25:293-300.
16. Gonzalez, F. J., C. L. Crespi, and H. V. Gelboin. 1991. cDNA-expressed human cytochrome p450s: a new age of molecular toxicology and human risk assessment. Mutat. Res. 247:113-127.
17. Sutler, T. R., Y. M. Tang, C. L. Hayes, Y. Y. Wo, E. W. Jabs, X. Li, H. Yin, C. W. Cody, and W. F. Greenlee. 1994. Complete cDNA sequence of a human dioxin-inducible mRNA identifies a new gene subfamily of cytochrome P450 that maps to chromosome 2. J. Biol Chem. 269:13092-13099.
18. Willey, J. C., E. Coy, C. Brolly, M. J. Utell, M. W. Frampton, J. Hammersley, W. G. Thilly, D. E. Olson, and K. Cairns. 1996. Xenobiotic metabolism enzyme gene expression in human bronchial epithelial and alveolar macrophage cells. Am. J. Respir. Cell Mol. Biol. 14:262-271.
19. Shou, M., K. R. Korzekwa, C. L. Crespi, F. J. Gonzalez, and H. V. Gelboin. 1994. The role of 12 cDNA-expressed human, rodent, and rabbit cytochromes p450 in the metabolism of benzo[a]pyrene and benzo[a]pyrene trans-7,8-dihydrodiol. Mol. Carcinog. 10:159-168.
20. Vanden Heuvel, J. P., G. C. Clark, C. L. Thompson, Z. McCoy, C. R. Miller, G. W. Lucier, and D. A. Bell. 1993. CYP1A1 mRNA levels as a human exposure marker: use of quantitative polymerase chain reaction to measure CYP1A1 expression in human peripheral blood lymphocytes. Carcinogenesis 14:2003-2006.
21. Anttila, S., E. Hietanen, H. Vainio, A.-M. Camus, H. V. Gelboin, S. S. Park, L. Heikkila, A. Karjalainen, and H. Bartsch. 1991. Smoking and peripheral type of cancer are related to high levels of pulmonary cytochrome P4501A in lung cancer patients. Int. J. Cancer 47:681-685.
22. Mullis, K. B., and F. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335-350.
23. Apostolakos, M. J., W. H. T. Schuermann, M. W. Frampton, M. J. Utell, and J. C. Willey. 1994. Measurement of gene expression by multiplex competitive polymerase chain reaction. Anal. Biochem. 213:277-284.
24. Omiecinski, C. J., C. A. Redlich, and P. Costa. 1990. Induction and developmental expression of cytochrome P4501A1 messenger RNA in rat and human tissues: detection by the polymerase chain reaction. Cancer Res. 50:4315-4321.
25. Willey, J. C., A. Broussoud, A. Sleemi, W. P. Bennett, P. Cerutti, and C. C. Harris. 1991. Immortalization of normal human bronchial epithelial cells by human papillomavirus 16 or 18. Cancer Res. 51:5370-5377.
26. Crespi, C. L. 1991. Expression of cytochrome P450 cDNAs in human B lymphoblastoid cells: applications to toxicology and metabolite analysis. Methods Enzymol. 206:123-133.
27. Beers, P. C., and J. L. Wittliff. 1975. Measurements of DNA and RNA in mammary gland homogenates by the ethidium bromide technique. Anal. Biochem. 63:433-441.
28. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chluroform extraction. Anal. Biochem. 162:156-159.
29. Celi, F. S., M. E. Zenilman, and A. R. Shuldiner. 1993. A rapid and versatile method to synthesize internal standards for competitive PCR. Nucleic Acids Res. 21:1047. (Abstr.)
30. Jaiswal, A. K., F. J. Gonzalez, and D. W. Nebert. 1985. Human dioxin-inducible cytochrome p1-450: complementary DNA and amino acid sequence. Science 228:80-83.
31. Yamano, S., P. T. Nhamburo, T. Aoyama, V. A. Meyer, T. Inaba, W. Kalow, H. V. Gelboin, O. W. McBride, and F. J. Gonzalez. 1989. cDNA cloning and sequence and cDNA-directed expression of human P450IIBI: identification of a normal and two variant cDNAs derived from CYP2B locus on chromosome 19 and differential expression of the IIB mRNAs in human liver. Biochemistry 28:7340-7348.
32. Skoda, R. C., A. Demierre, O. W. McBride, F. J. Gonzalez, and U. A. Meyer. 1988. Human microsomal xenobiotic epoxide hydrolase: cDNA sequence, cDNA directed expression in COS-1 cells and chromosomal localization. J. Biol. Chem. 263:1549-1554.
33. Yamano, S., T. Aoyama, O. W. McBride, J. P. Hardwick, H. V. Gelboin, and F. J. Gonzalez. 1989. Human NADPH-P450 oxidoreduclase: complementary DNA cloning, sequence and vaccinia virus-mediated expression and localization of the CYPOR gene to chromosome 7. Mol. Pharmacol. 36:83-88.
34. Burke, M. D., R. A. Prough, and R. T. Mayer. 1477. Characteristics of a microsomal cytochrome P-448-mediated reaction ethoxyresorufinO-de-ethylation. Drug Metab. Disp. 5:1-8.
35. Phillips, A. H., and R. G. Langdon. 1962. Hepatic triphosphopyridine nucleotide cytochrome C reductase: isolation, characterization and kinetic studies. J. Biol. Chem. 237:2652-2660.
36. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics 11:1-42.
37. Petruzzelli, S., A.-M. Camus, L. Carrozzi, L. Ghelarducci, M. Rindi, G. Menconi, C. A. Angeletti, M. Ahotupa, E. Hietanen, A. Aitio, R. Caracci, H. Bartsch, and C. Giuntini. 1988. Long-lasting effects of tobacco smoking on pulmonary drug-metabolizing enzymes: a case control study on lung cancer patients. Cancer Res. 48:4695-4700.
38. Stauber, K., J. D. Laskin, K. J. Yurkow, P. K. Thomas, D. L. Laskin, and A. H. Conney. 1995. flow cytometry reveals subpopulations of murine epidermal cells that are refractory to induction of cytochrome P-4501A1 by β-napthoflavone. J. Pharmacol. Exp. Ther. 273:967-976.
39. McLemore, T. L., S. Adelberg, M. C. Liu, N. A. McMahon, S. Jin Yu, W. C. Hubbard, M. Czerwinski, T. G. Wood, R. Storeng, R. A. Lubet, J. C. Eggleston, M. R. Boyd, and R. N. Hines. 1990. Expression of CYP1A1 gene in patients with lung cancer: evidence for cigarette smoke-induced gene expression in normal lung tissue and for altered gene regulation in primary pulmonary carcinomas. J. Natl. Cancer Inst. 82:1333-1339.
40. Harris, C. C., I. C. Hsu, G. D. Stoner, B. F. Trump, and J. K. Selkirk. 1978. Human pulmonary alveolar macrophages metabolise benzo[a]pyrene to proximate and ultimate mutagens. Nature 272:633-634.
41. Cantrell, F. T., G. A. Warr, D. L. Busbee, and R. R. Martin. 1973. Induction of aryl hydrocarbon hydroxylase in human pulmonary alveolar macrophages by cigarette smoking. J. Clin. Invest. 52:1881-1884.
42. Czerwinski, M., T. L. Mclemore, H. V. Gelboin, and F. J. Gonzalez. 1994. Quantification of CYP2B7, CYP4B1, and CYPOR messenger RNAs in normal human lung and lung tumors. Cancer Res. 54:1085-1091.
43. Vanden Heuvel, J. P., G. C. Clark, M. C. Kohn, A. M. Tritscher, W. F Greenlee, G. W. Lucier, and D. A. Bell. 1994. Dioxin-responsive genes: examination of dose-response relationships using quantitative reverse transcriptase-polymerase chain reaction. Cancer Res. 54:62-68.