Induction and regulation of xenobiotic-metabolizing cytochrome P450s in the human A549 lung adenocarcinoma cell line

American Journal of Respiratory Cell and Molecular Biology

Volumn 22, Issue 3, 2000, Pages 360-366

  Hukkanen, Janne ^a   Lassila, Arja ^a   Päivärinta, Katja ^a   Valanne, Susanna ^a   Sarpo, Sari ^a   Hakkola, Jukka ^a   Pelkonen, Olavi ^a   Raunio, Hannu ^a  

^a UNIVERSITY OF OULU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

2,3,7,8 TETRACHLORODIBENZO PARA DIOXIN; CALYCULIN A; CARCINOMA CELL; CYTOCHROME P450; DEXAMETHASONE; ENZYME INDUCTION; ENZYME INHIBITION; ENZYME INHIBITOR; EPITHELIUM CELL; GENISTEIN; HUMAN CELL; LUNG; MESSENGER RNA; OKADAIC ACID; PHENOBARBITAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAUROSPORINE;

EID: 0034046572     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.22.3.3845     Document Type: Article

References (65)

1. 1. Guengerich, F. P. 1993. Bioactivation and detoxication of toxic and carcinogenic chemicals. Drug Metab. Dispos. 21:1-6.
2. 2. Nelson, D. R., L. Koymans, T. Kamataki, J. J. Stegeman, R. Feyereisen, D. J. Waxman, M. R. Waterman, O. Gotoh, M. J. Coon, R. W. Estabrook, I. C. Gunsalus, and D. W. Nebert. 1996. P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6:1-42.
3. 3. Raunio, H., K. Husgafvel-Pursiainen, S. Anttila, E. Hietanen, A. Hirvonen, and O. Pelkonen. 1995. Diagnosis of polymorphisms in carcinogen-activating and inactivating enzymes and cancer susceptibility [review]. Gene 159: 113-121.
4. 4. Nebert, D. W., R. A. McKinnon, and A. Puga. 1996. Human drug-metabolizing enzyme polymorphisms: effects on risk of toxicity and cancer. DNA Cell Biol. 15:273-280.
5. 5. Autrup, H. 1990. Carcinogen metabolism in cultured human tissues and cells. Carcinogenesis 11:707-712.
6. 6. Denissenko, M. F., A. Pao, M. Tang, and G. P. Pfeifer. 1996. Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in P53. Science 274:430-432.
7. 7. Omiecinski, C. J., C. A. Redlich, and P. Costa. 1990. Induction and developmental expression of cytochrome P450IA1 messenger RNA in rat and human tissues. Cancer Res. 50:4315-4321.
8. 8. McLemore, T. L., S. Adelberg, M. C. Liu, N. A. McMahon, S. J. Yu, W. C. Hubbard, M. Czerwinski, T. G. Wood, R. Storeng. R. A. Lubet, J. C. Eggleston, M. R. Boyd, and R. N. Hines. 1990. Expression of CYP1A1 gene in patients with lung cancer: evidence for cigarette smoke-induced gene expression in normal lung tissue and for altered gene regulation in primary pulmonary carcinomas. J. Natl. Cancer Inst. 83:1333-1339.
9. 9. Anttila, S., E. Hietanen, H. Vainio, A.-M. Camus, H. V. Gelboin, S. S. Park, L. Heìkkilä, A. Karjalainen, and H. Bartsch. 1991. Smoking and peripheral type of cancer are related to high levels of pulmonary cytochrome P450IA in lung cancer patients. Int. J. Cancer 47:681-685.
10. 10. Willey, J. C., E. Coy, C. Brolly, M. J. Utell, M. W. Frampton, J. Hammersley, W. G. Thilly, D. Olson, and K. Cairns. 1996. Xenobiotic metabolism gene expression in human bronchial epithelial and alveolar macrophage cells. Am. J. Respir. Cell Mol. Biol. 14:262-271.
11. 11. Willey, J. C., E. L. Coy, M. W. Frampton, A. Torres, M. J. Apostolakos, G. Hoehn, W. H. Schuermann, W. G. Thilly, D. E. Olson, J. R. Hammersley, C. L. Crespi, and M. J. Utell. 1997. Quantitative RT-PCR measurement of cytochromes p450 1A1, 1B1, and 2B7, microsomal epoxide hydrolase, and NADPH oxidoreductase expression in lung cells of smokers and non-smokers. Am. J. Respir. Cell Mol. Biol. 17:114-124.
12. 12. Macé, K., E. D. Bowman, P. Vautravers, P. G. Shields, C. C. Harris, and A. M. A. Pfeifer. 1998. Characterization of xenobiotic-metabolizing enzyme expression in human bronchial mucosa and peripheral lung tissue. Eur. J. Cancer 34:914-920.
13. 13. Saarikoski, S. T., K. Husgafvel-Pursiainen, A. Hirvonen, H. Vainio, F. J. Gonzalez, and S. Anttila. 1998. Localization of CYP1A1 mRNA in human lung by in situ hybridization: comparison with immunohistochemical findings. Int. J. Cancer 77:33-39.
14. 14. Kivistö, K. T., E.-U. Griese, P. Fritz, A. Linder, J. Hakkola, H. Raunio, P. Beaune, and H. K. Kroemer. 1996. Expression of cytochrome P4503A enzymes in human lung: a combined RT-PCR and immunohistochemical analysis of normal tissue and lung tumors. Naunyn-Schmiedeberg Arch. Pharmacol. 353:207-212.
15. 15. Anttila, S., J. Hukkanen, J. Hakkola, T. Stjernvall, P. Beaune, R. J. Edwards, A. R. Boobis, O. Pelkonen, and H. Raunio. 1997. Expression and localization of CYP3A4 and CYP3A5 in human lung. Am. J. Respir. Cell Mol. Biol. 16: 242-249.
16. 16. Pelkonen, O., J. Mäenpää, P. Taavitsainen, A. Rautio, and H. Raunio. 1998. Inhibition and induction of human cytochrome P450 (CYP) enzymes. Xenobiotica 28:1203-1253.
17. 17. Raunio, H., M. Pasanen, J. Mäenpää, J. Hakkola, and O. Pelkonen. 1995. Expression of extrahepatic cytochrome P450 in humans. In Advances in Drug Metabolism in Man. G. M. Pacifici and G. N. Fracchia, editors. European Commission, Office for Official Publications of the European Communities, Luxembourg. 234-287.
18. 18. Wrighton, S. A., B. J. Ring, P. B. Watkins, and M. VandenBranden. 1989. Identification of a polymorphically expressed member of the human cytochrome P-450III family. Mol. Pharmacol. 86:97-105.
19. 19. Schuetz, E. G., J. D. Schuetz, S. C. Strom, M. T. Thompson, R. A. Fisher, D. T. Molowa, D. Li, and P. S. Guzelian. 1993. Regulation of human liver cytochromes P-450 in family 3A in primary and continuous culture of human hepatocytes. Hepatology 18:1254-1262.
20. 20. Lieber, M., B. Smith, A. Szakal, W. Nelson-Rees, and G. Todaro. 1976. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 17:62-70.
21. 21. Döhr, O., R. Sinning, C. Vogel, P. Munzel, and J. Abel. 1997. Effect of transforming growth factor-β1 on expression of aryl hydrocarbon receptor and genes of Ah gene battery: clues for independent down-regulation in A549 cells. Mol. Pharmacol. 51:703-710.
22. 22. Foster, K. A., C. G. Oster, M. M. Mayer, M. L. Avery, and K. L. Audus. 1998. Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism. Exp. Cell. Res. 243:359-366.
23. 23. Feldman, G., J. Remsen, K. Shinohara, and P. Cerutti. 1978. Excisability and persistence of benzo(a)pyrene DNA adducts in epithelioid human lung cells. Nature 274:796-798.
24. 24. Hukkanen, J., J. Hakkola, S. Anttila, R. Piipari, A. Karjalainen, O. Pelkonen, and H. Raunio. 1997. Detection of mRNA encoding xenobiotic-metabolizing cytochrome P450s in human broncho-alveolar macrophages and peripheral blood lymphocytes. Mol. Carcinog. 20:224-230.
25. 25. Hakkola, J., M. Pasanen, O. Pelkonen, J. Hukkanen, S. Evisalmi, S. Anttila, A. Rane, M. Mäntylä, R. Purkunen, S. Saarikoski, M. Tooming, and H. Raunio. 1997. Expression of CYP1B1 in human adult and fetal tissues and differential inducibility of CYP1B1 and CYP1A1 by Ah receptor ligands in human placenta and cultured cells. Carcinogenesis 18:391-397.
26. 26. Hakkola, J., M. Pasanen, J. Hukkanen, O. Pelkonen, J. Mäenpää, R. J. Edwards, A. R. Boobis, and H. Raunio. 1996. Expression of xenobiotic-metabolizing cytochrome P450 forms in human full-term placenta. Biochem. Pharmacol 51:403-411.
27. 27. Hakkola, J., H. Raunio, R. Purkunen, O. Pelkonen, S. Saarikoski, T. Crestoil, and M. Pasanen. 1996. Detection of cytochrome P450 gene expression in human placenta in first trimester of pregnancy. Biochem. Pharmacol. 52:379-383.
28. 28. Koskela, S., J. Hakkola, J. Hukkanen, O. Pelkonen, M. Sorri, A. Saranen, S. Anttila, P. Fernandez-Salguero, F. J. Gonzalez, and H. Raunio. 1999. Expression of CYP2A genes in human liver and extrahepatic tissues. Biochem. Pharmacol. 57:1407-1413.
29. 29. Jin, C.-F., M. Mata, and D. J. Fink. 1994. Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR. PCR Methods Applic. 3:252-255.
30. 30. Kivistö, K. T., E.-U. Griese, T. Stuven, P. Fritz, G. Freidel, H. K. Kroemer, and U. M. Zanger. 1997. Analysis of CYP2D6 expression in human lung: implications for the association between CYP2D6 activity and susceptibility to lung cancer. Pharmacogenetics 7:295-302.
31. 31. Mori, M., F. Tezuka, R. Chiba, Y. Funae, M. Watanabe, T. Nukiwa, and T. Takahashi. 1996. A typical adenomatous hyperplasia and adenocarcinoma of the human lung: their heterology in form and analogy in immunohistochemical characteristics. Cancer 77:665-674.
32. 32. Kivistö, K. T., P. Fritz, A. Linder, G. Friedel, P. Beaune, and H. K. Kroemer. 1995. Immunohistochemical localization of cytochrome P450 3A in human pulmonary carcinomas and normal bronchial tissue. Histochemistry 103:25-29.
33. 33. Devereux, T. R., T. E. Massey, M. R. Van Scott, J. Yankaskas, and J. R. Fouts. 1986. Xenobiotic metabolism in human alveolar type II cells isolated by centrifugal elutriation and density gradient centrifugation. Cancer Res. 46:5438-5443.
34. 34. McLemore, T. L., S. Adelberg, M. Czerwinski, W. C. Hubbard, S. J. Yu, R. Storeng.T. G. Wood, R. N. Hines, and M. R. Boyd. 1989. Altered regulation of the cytochrome P4501A1 gene: novel inducer-independent gene expression in pulmonary carcinoma cell lines. J. Natl. Cancer Inst. 81:1787-1794.
35. 35. Urani, C., M. Doldi, S. Crippa, and M. Camatini. 1998. Human-derived cell lines to study xenobiotic metabolism. Chemosphere 37:2785-2795.
36. 36. Rämet, M., K. Castrén, K. Järvinen, K. Pekkala, T. Turpeenniemi-Hujanen, Y. Soini, P. Pääkkö, and K. Vähäkangas. 1995. p53 Protein expression is correlated with benzo(a)pyrene-DNA adducts in carcinoma cell lines. Carcinogenesis 16:2117-2124.
37. 1 coregulates mRNA expression of aryl hydrocarbon receptor and cell-cycle-regulating genes in human cancer cell lines. Biochem. Biophys. Res. Commun. 241: 86-91.
38. 1 inhibits TCDD-induced cytochrome P450IA1 expression in human lung cancer A549 cells. Arch. Toxicol. 68:303-307.
39. 39. Czerwinski, M., T. L. McLemore, H. V. Gelboin, and F. J. Gonzalez. 1994. Quantitation of CYP2B7, CYP4B1, and CYPOR messenger RNAs in normal human lung and lung tumors. Cancer Res. 54:1085-1091.
40. 1 chrome P450 enzymes involved in the oxidation of xenobiotic chemicals in human fetal livers and adult lungs. Drug Metab. Dispos. 24:515-522.
41. 41. Guidice, J.-M. L., D. Marez, N. Sabbagh, M. Legrand-Andreoletti, C. Spire, E. Alcaide, J.-J. Lafitte, and F. Broly. 1997. Evidence for CYP2D6 expression in human lung. Biochem. Biophys. Res. Commun. 241:79-85.
42. 42. Huang, Z., M. J. Fasco, S. Spivack, and L. S. Kaminsky. 1997. Comparisons of CYP2D messenger RNA splice variant profiles in human lung tumors and normal tissues. Cancer Res. 57:2589-2592.
43. 43. Wheeler, C. W., S. A. Wrighton, and T. M. Guenthner. 1992. Detection of human lung cytochrome P450 that are immunochemically related to cytochrome P450IIE1 and cytochrome P450IIIA. Biochem. Pharmacol. 44:911-917.
44. 44. Botto, F., E. Seree, S. E. Khyari, G. de Sousa, A. Massacrier, M. Placidi, P. Cau, W. Pellet, R. Rahmani, and Y. Barra. 1994. Tissue-specific expression and methylation of the human CYP2E1 gene. Biochem. Pharmacol. 48: 1095-1103.
45. 45. Kivistö, K. T., A. Linder, G. Friedel, P. Beaune, C. Belloc, H. K. Kroemer, and P. Fritz. 1995. Immunohistochemical localization of cytochrome P450 2E1 in human pulmonary carcinoma and normal bronchial tissue. Virchows Arch. 426:243-247.
46. 46. Nhamburo, P. T., S. Kimura, O. W. McBride, C. A. Kozak, H. V. Gelboin, and F. J. Gonzalez. 1990. The human CYP2F gene family: identification of a cDNA encoding a new cytochrome P450, cDNA-directed expression, and chromosome mapping. Biochemistry 29:5491-5499.
47. 47. Nhamburo, P. T., F. J. Gonzalez, O. W. McBride, H. V. Gelboin, and S. Kimura. 1989. Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping. Biochemistry 28:8060-8066.
48. 48. Schuetz, E. G., W. T. Beck, and J. D. Schuetz. 1996. Modulators and substrates of P-glycoprotein and cytochrome P4503A coordinately up-regulate these proteins in human colon carcinoma cells. Mol. Pharmacol. 49: 311-318.
49. 49. Schuetz, J. D., E. G. Schuetz, J. V. Thottassery, P. S. Guzelian, S. Strom, and D. Sun. 1996. Identification of a novel dexamethasone responsive enhancer in the human CYP3A5 gene and its activation in human and rat liver cells. Mol. Pharmacol. 49:63-72.
50. 50. Blumberg, B., W. Sabbagh, Jr., H, Juguilon, J. Bolado, Jr., C. M. van Meter, E. S. Ong, and R. M. Evans. 1998. SXR, a novel steroid and xenobiotic-sensing nuclear receptor. Genes Dev. 12:3195-3205.
51. 51. Gradin, K., M. L. Whitelaw, R. Toftgård, L. Poellinger, and A. Berghard. 1994. A tyrosine kinase-dependent pathway regulates ligand-dependent activation of the dioxin receptor in human keratinocytes. J. Biol. Chem. 269:23800-23807.
52. 52. Kikuchi, H., A. Hossain, H. Yoshida, and S. Kobayashi. 1998. Induction of cytochrome P-450 1A1 by omeprazole in human HepG2 cells is protein tyrosine kinase-dependent and is not inhibited by α-naphthoflavone. Arch. Biochem. Biophys. 358:351-358.
53. 53. Backlund, M., I. Johansson, S. Mkrtchian, and M. Ingelman-Sundberg. 1997. Signal transduction-mediated activation of the aryl hydrocarbon receptor in rat hepatoma H4IIE cells. J. Biol. Chem. 272:31755-31763.
54. 54. Carrier, F., R. A. Owens, D. W. Nebert, and A. Puga. 1992. Dioxin-dependent activation of murine Cypla-1 gene transcription requires protein kinase C-dependent phosphorylation. Mol. Cell. Biol. 12:1856-1863.
55. 55. Gradin, K., R. Toftgård, and A. Berghard. 1995. Differential effects of a topoisomerase I inhibitor on dioxin inducibility and high-level expression of the cytochrome P450IA1 gene. Mol. Pharmacol. 48:610-615.
56. 56. Wo. Y.-Y. P., J. Stewart, and W. F. Greenlee. 1997. Functional analysis of the promoter for the human CYP1BI gene. J. Biol. Chem. 272:26702-26707.
57. 57. Berghard, A., K. Gradin, I. Pongratz, M. Whitelaw, and L. Poellinger. 1993. Cross-coupling of signal transduction pathways: the dioxin receptor mediates induction of cytochrome P-450IA1 expression via a protein kinase C-dependent mechanism. Mol. Cell. Biol. 13:677-689.
58. 58. Reiners, J. J., A. Schöller, P. Bischer, A. R. Cantu, and A. Pavone. 1993. Suppression of cytochrome P450 Cyp Ia-I induction in murine hepatoma 1c1c7 cells by 12-O-tetradecanoylphorbol-13-acetate and inhibitors of protein kinase C. Arch. Biochem. Biophys. 301:449-454.
59. 59. Chen, Y.-H., and R. H. Tukey. 1996. Protein kinase C modulates regulation of the CYP1AI gene by the aryl hydrocarbon receptor. J. Biol. Chem. 271: 26261-26266.
60. 60. Xiao, G.-H., C. Falkner, Y. Xie, R. G. Lindahl, and R. A. Prough. 1997. cAMP-dependent negative regulation of rat aldehyde dehydrogenase class 3 gene expression. J. Biol. Chem. 272:3238-3245.
61. 61. Long, W. P., M. Pray-Grant, J. C. Tsai, and G. H. Perdew. 1998. Protein kinase C activity is required for aryl hydrocarbon receptor pathway-mediated signal transduction. Mol. Pharmacol. 53:691-700.
62. 62. Schafer, M. W., B. V. Madhukar, H. I. Swanson, K. Tullis, and M. S. Denison. 1993. Protein kinase C is not involved in Ah receptor transformation and DNA binding. Arch. Biochem. Biophys. 307:267-271.
63. 63. Li, S.-Y., and J. J. Dougherty. 1997. Inhibitors of serine/threonine-specific protein phosphatases stimulate transcription by the Ah receptor/Arnt dimer by affecting a step subsequent to XRE binding. Arch. Biochem. Biophys. 340: 73-82.
64. 64. Pongratz, I., P. E. Stromstedt, G. G. Mason, and L. Poellinger. 1991. Inhibition of the specific DNA binding activity of the dioxin receptor by phosphatase treatment. J. Biol. Chem. 266:16813-16817.
65. 65. Mahon, M. J., and T. A. Gasiewicz. 1995. Ah receptor phosphorylation: localization of phosphorylation sites to the C-terminal half of the protein. Arch. Biochem. Biophys. 318:166-174.