Construction and characterization of a novel recombinant single-chain variable fragment antibody against Western equine encephalitis virus

Hybridoma

Volumn 19, Issue 1, 2000, Pages 1-13

  Long, Melissa C ^a   Jager, Scott ^b   Mah, Dave C W ^b   Jebailey, Lellean ^a   Mah, Maria A ^b   Masri, Saad A ^c   Nagata, Les P ^a  

^a Medical Countermeasures Section   (Canada)

^b Canada West Biosciences Inc   (Canada)

^c Canadian Food Inspection Agency   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENT; PROTEIN G; RECOMBINANT ANTIBODY;

AMINO ACID SEQUENCE; ANTIBODY RESPONSE; ANTIGEN ANTIBODY REACTION; ARTICLE; CONFORMATION; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOTHERAPY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; WESTERN EQUINE ENCEPHALOMYELITIS ALPHAVIRUS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; BINDING SITES, ANTIBODY; CLONING, MOLECULAR; ENCEPHALITIS VIRUS, WESTERN EQUINE; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

ALPHAVIRUS; EQUIDAE; RNA VIRUSES; WESTERN EQUINE ENCEPHALOMYELITIS VIRUS;

EID: 0034026297     PISSN: 0272457X     EISSN: None     Source Type: Journal    
DOI: 10.1089/027245700315743     Document Type: Article

References (53)

1. Strauss JH, and Strauss EG: The Alphaviruses: Gene expression, replication, and evolution. Microbiol Rev 1994;58:491-562.
2. Strauss JH, Strauss EG, and Kuhn RJ: Budding of alphaviruses. Trends Microbiol 1955;3:346-350.
3. Schlesinger S, and Schlesinger MJ: Togaviridae: The viruses and their replication. In: Fields Virology, 3rd ed. Fields BN, Knipe DM, and Howley PM (Eds.). Raven Publishers, Philadelphia, 1996, pp. 843-898.
4. Johnston RE, and Peters CJ: Alphaviruses. In: Fields Virology, 3rd ed. Fields BN, Knipe DM, and Howley PM (Eds.). Raven Publishers, Philadelphia, 1996, pp. 843-898.
5. 2 glycoprotein of semiliki forest virus can protect mice from lethal encephalitis. J Gen Virol 1983;64:1405-1408.
6. Mathews JH, and Roehrig JT: Determination of the protective epitopes on the glycoproteins of venezuelan equine encephalomyelitis virus by passive transfer of monoclonal antibodies. J Immunol 1982;129:2763-2767.
7. Yamamoto K: Properties of monospecific antibodies to the glycoprotein of western equine encephalitis virus. Microbiol Immunol 1986;30:343-351.
8. Griffin D, Levine B, Tyor W, Ubol S, and Despres P: The role of antibody in recovery from alphavirus encephalitis. Immunol Rev 1997;159:155-161.
9. Wright A, Shin S-U, and Morrison SL: Genetically engineered antibodies. Crit Rev Immunol 1992;12:125-168.
10. Hayden MS, Gilliland LK, and Ledbetter JA: Antibody engineering. Curr Opin Immunol 1997;9:201-212.
11. Verma R, Boleti E, and George AJT: Antibody engineering: Comparison of bacterial, yeast, insect, and mammalian expression systems. J Immunol Methods 1998;216:165-181.
12. Winter G, and Milstein C: Man-made antibodies. Nature 1991;349:293-299.
13. Pantoliano MW, Bird RE, Johnson S, Asel ED, Dodd SW, Wood JF, and Hardman KD: Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli. Biochemistry 1991;30:10117-10125.
14. Jiang W, Bonnert TP, Venugopal K, and Gould EA: A single chain antibody fragment expressed in bacteria neutralizes tick-borne flaviviruses. Virology 1994;200:21-28.
15. Mason P, Berinstein A, Baxt B, Parsells R, Kang A, and Rieder E: Cloning and expression of a single-chain antibody fragment specific for foot-and-mouth disease virus. Virology 1996;224:548-554.
16. Lamarre A, Yu MWN, Chagnon F, and Talbot PJ: A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice. Eur J Immunol 1997;27:3447-3455.
17. Raag R, and Whitlow M: Single-chain Fvs. FASEB J 1995;9:73-80.
18. Alvi AZ, Stadnyk LL, Nagata LP, Fulton RE, Bader DE, Roehrig JT, and Suresh MR: Development of a functional monoclonal single-chain variable fragment antibody against venezuelan equine encephalitis virus. Hybridoma 1999;18:413-421.
19. Xu B, Kriangkum J, Nagata LP, Fulton RE, and Suresh MR: Generation and characterization of a single chain Fv specific against western equine encephalitis virus. Hybridoma 1999;18:315-323.
20. Guirakhoo F, Catalan J, Monath T, and Weltzin R: Cloning, expression and functional activities of a single chain antibody fragment directed to fusion protein of respiratory syncytial virus. Immunotechnology 1996;2:219-228.
21. Muller BH, Lafay F, Demangel C, Perrin P, Tordo N, Flamand A, Lafaye P, and Guesdon JL: Phage-displayed and soluble mouse scFv fragments neutralize rabies virus. J Virol Methods 1997;67:221-233.
22. Lewis AP, Lemon SM, Barber KA, Murphy P, Parry NR, Peakman TC, Sims MJ, Worden J, and Crowe JS: Rescue, expression, and analysis of a neutralizing human anti-hepatitis A virus monoclonal antibody. J Immunol 1993;151:2829-2838.
23. Mah D, Masri S, Mah M, and Jager S: Canada West Biosciences, Calgary, Alberta, Canada, unpublished data.
24. Nagata LP, Alvi AZ, and Ludwig G: Defence Research Establishment Suffield, Medicine Hat, Alberta, Canada, unpublished data.
25. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
26. Towbin H, Staehelin T, and Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;9:4350-4354.
27. Rice SA, Long MC, Lam V, and Spencer CA: RNA polymerase II is aberrantly phosphorylated and localized to viral replication compartments following herpes simplex virus infection. J Virol 1994;68:988-1001.
28. Tang PM, Foltz LA, Mahoney WC, and Schueler PA: A high affinity digoxin-bindin g protein displayed on M13 is functionally identical to the native protein. J Biol Chem 1995;270:7829-7835.
29. γ1 gene. Nucleic Acids Res 1982;10:4071-4079.
30. Ellison J, and Hood L: Linkage and sequence homology of two human immunoglobulin gamma heavy chain constant region genes. Proc Natl Acad Sci USA 1982;79:1984-1988.
31. Paterson T, Innes J, McMillan L, Downing I, and Carter MC: Variation in IgG1 heavy chain allotype does not contribute to differences in biological activity of two human anti-Rhesus (D) monoclonal antibodies. Immunotechnology 1998;4:37-47.
32. Turner M: Antibodies and their receptors. In: Immunology, 5th ed. Roitt I, Brostoff J, and Male D (Eds.). Mosby, London, 1998, pp. 71-82.
33. Walport M: Complement. In: Immunology, 5th ed. Roitt I, Brostoff J, and Male D (Eds.). Mosby, London, 1998, pp. 43-59.
34. Tan LK, Shopes RJ, Oi VT, and Morrison SL: Influence of the hinge region on complement activation, C1q binding, and segmental flexibility in chimeric human immunoglobulins. Proc Natl Acad Sci USA 1990;87:162-166.
35. Tao MH, Canfield SM, and Morrison SL: The differential ability of human IgG1 and IgG4 to activate complement is determined by the COOH-terminal sequence of the CH2 domain. J Exp Med 1991;173:1025-1028.
36. Shopes B, Weetall M, Holowka D, and Baird B: Recombinant human IgG1-murine IgE chimeric Ig. Construction, expression, and binding to human Fc gamma receptors. J Immunol 1990;145:3842-3848.
37. Canfield SM, and Morrison SL: The binding affinity of human IgG for its high affinity Fc receptor is determined by multiple amino acids in the CH2 domain and is modulated by the hinge region. J Exp Med 1991;173:1483-1491.
38. Jin BR, Ryu CJ, Park SS, Namgung U, Hong HJ, and Han MH: Cloning, expression, and characterization of murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus. Mol Immunol 1993;30:1647-1654.
39. Roguska MA, Pedersen JT, Keddy CA, Henry AH, Searle SJ, Lambert JM, Goldmacher VS, Blattler WA, Rees AR, and Guild BC: Humanization of murine monoclonal antibodies through viable domain resurfacing. Proc Natl Acad Sci USA 1994;91:969-973.
40. Vaughan TJ, Williams AJ, Pritchard K, Osbourn JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J, and Johnson KS: Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat Biotechnol 1996;14:309-314.
41. Crameri A, Cwirla S, and Stemmer WPC: Construction and evolution of antibody-phage libraries by DNA shuffling. Nat Med 1996;2:100-102.
42. Vaughan TJ, Osbourn JK, and Tempest PR: Human antibodies by design. Nat Biotechnol 1998;16:535-539.
43. Edelman GM, and Gall WE: The antibody problem. Annu Rev Biochem 1969;38:415-466.
44. Hu S, Shively L, Raubitschek A, Sherman M, Williams LE, Wong JY, Shively JE, and Wu AM: Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts. Cancer Res 1996;56:3055-3061.
45. Coloma MJ, and Morrison SL: Design and production of novel tetravalent bispecific antibodies. Nat Biotechnol 1997;15:159-163.
46. McGregor DP, Molloy PE, Cunningham C, and Harris WJ: Spontaneous assembly of bivalent single chain antibody fragments in Escherichia coli. Mol Immunol 1994;31:219-226.
47. Gilliland LK, Norris NA, Marquardt H, Tsu TT, Hayden MS, Neubauer MG, Yelton DE, Mittler RS, and Ledbetter JA: Rapid and reliable cloning of antibody variable regions and generation of recombinant single chain antibody fragments. Tissue Antigens 1996;47:1-20.
48. Tao MH, and Morrison SL: Studies of aglycosylated chimeric mouse-human IgG. Role of carbohydrate in the structure and effector functions mediated by the human IgG constant region. J Immunol 1989;143:2595-2601.
49. Shaw DR, Khazaeli MB, and LoBuglio AF: Mouse/human chimeric antibodies to a tumor-associated antigen: Biologic activity of the four human IgG subclasses. J Natl Cancer Inst 1988;80:1553-1559.
50. Wright A, and Morrison SL: Effect of glycosylation on antibody function: Implications for genetic engineering. Trends Biotechnol 1997;15:26-32.
51. Jefferis R and Lund J: Glycosylation of antibody molecules: structural and functional significance. Chem Immunol 1997;65:111-128.
52. Hsieh P, Rosner MR, and Robbins PW: Selective cleavage by endo-β-acetylglucosaminidase 1γ at individual glycosylation sites of Sindbis virion envelope glycoproteins. J Biol Chem 1983;259:2555-2561.
53. Cao Y, and Suresh MR: Bispecific antibodies as novel bioconjugates. Bioconjug Chem 1998;9:635-644.