Measurement of sirolimus in whole blood using high-performance liquid chromatography with ultraviolet detection

Clinical Therapeutics

Volumn 22, Issue SUPPL. B, 2000, Pages

  Holt, David W ^a   Lee, Terry ^b   Johnston, Atholl ^b  

^a ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

^b ST BARTHOLOMEW S HOSPITAL   (United Kingdom)

Author keywords

High performance liquid chromatography (HPLC); Rapamycin; Sirolimus; Therapeutic drug monitoring

Indexed keywords

CYCLOSPORIN; IMMUNOSUPPRESSIVE AGENT; RAPAMYCIN;

ARTICLE; BLOOD SAMPLING; DRUG BLOOD LEVEL; DRUG MONITORING; GRAFT REJECTION; HUMAN; IMMUNOSUPPRESSIVE TREATMENT; MASS SPECTROMETRY; MICROPARTICLE ENZYME IMMUNOASSAY; ORGAN TRANSPLANTATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0034023133     PISSN: 01492918     EISSN: 1879114X     Source Type: Journal    
DOI: 10.1016/S0149-2918(00)89021-9     Document Type: Article

References (11)

1. Sehgal SN. Rapamune (sirolimus, rapamycin): An overview and mechanism of action. Ther Drug Monit. 1995;17:660-665.
2. Groth CG, Backman L, Morales JM, et al. Sirolimus (rapamycin)-based therapy in human renal transplantation: Similar efficacy and different toxicity compared with cyclosporine. Sirolimus European Renal Transplant Study Group. Transplantation. 1999;67:1036-1042.
3. Trepanier DJ, Gallant H, LeGatt DF, Yatscoff RW. Rapamycin: Distribution, pharmacokinetics and therapeutic range investigations: An update. Clin Biochem. 1998;31:345-351.
4. Yatscoff RW, Boeckx R, Holt DW, et al. Consensus guidelines for therapeutic drug monitoring of rapamycin: Report of the consensus panel. Ther Drug Monit. 1995; 17:676-680.
5. Streit F, Christians U, Schiebel HM, et al. Structural identification of three metabolites and a degradation product of the macrolide immunosuppressant sirolimus (rapamycin) by electrospray-MS/MS after incubation with human liver microsomes. Drug Metab Dispos. 1996;24:1272-1278.
6. Jones K, Saadat-Lajevardi S, Lee T, et al. An immunoassay for the measurement of sirolimus. Clin Ther. 2000;22(Suppl B): B49-B61.
7. Hollis S. Analysis of method comparison studies. Ann Clin Biochem. 1996;33:1-4.
8. Svensson JO, Brattstrom C, Sawe J. Determination of rapamycin in whole blood by HPLC. Ther Drug Monit. 1997;19:112-116.
9. Napoli KL, Kahan BD. Sample clean-up and high-performance liquid chromatographic techniques for measurement of whole blood rapamycin concentrations. J Chromatogr B Biomed Appl. 1994;654: 111-120.
10. Napoli KL, Kahan BD. Routine clinical monitoring of sirolimus (rapamycin) whole-blood concentrations by HPLC with ultraviolet detection. Clin Chem. 1996;42:1943-1948.
11. Ferron GM, Jusko WJ. Species differences in sirolimus stability in humans, rabbits, and rats. Drug Metab Dispos. 1998;26:83-84.